Cpm (BC100404) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1110060I01Rik; 5730456K23Rik; AA589379; E030045M14Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207070 representing BC100404 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGCGCGCGCCTGTGGCTGGGGCTTCTGCTGCCCGTGGTGGCCGCCCTGGATTTCCGATATCACC ACCAGGAAGGGATGGAGGCGTTCCTAAAGAGCGTGGCTCAGAACTACAGTTCGATCACGCACTTGCACAG CATCGGGAAATCTGTGAGAGGTAGAAACCTGTGGGTTCTCGTTGTGGGGCAGACTCCAAAGGAACACAGA GTCGGGATTCCAGAATTCAAATACGTGGCGAATATGCACGGAGATGAGACTGTAGGGCGAGAACTGCTGC TCCACCTGATCGACTATCTGGTGAGCAGTTACAGGAAAGACCCGGAAATCACGCATCTGATTGACAGCAC CCGGATCCACATCATGCCTTCCATGAACCCCGATGGATTCGAAGCCGTCCAGAAGCCCGACTGTTACTAC AGCAATGGCAGGGAAAACTACAATAATTATGACCTGAACAGAAACTTTCCTGATGCCTTTGAGAATAACA ATGTAACAAAGCAACCTGAGACTCTGGCAATCATGGAGTGGCTGAAAACGGAAACATTTGTCCTCTCTGC GAATCTCCATGGGGGTGCCCTGGTGGCCAGTTACCCCTTTGATAATGGCGTACAAGCTACGGGGACACTG TTGTCCCGAAGCTTAACCCCGGACGATGACGTTTTCCAACACCTGGCCTACACCTACGCCTCCCGGAATC CCAACATGACAAAAGGAGATCAGTGTAAAAACAAGAGGAACTTCCCCAACGGGATTATCAACGGCTACTC CTGGTACCCACTGCAAGGGGGAATGCAAGATTACAACTACATCTGGGCCCAGTGCTTTGAAATTACCCTG GAGCTGTCGTGCTGTAAATATCCTCGCGAGGAGAAGCTGCCGCTCTTTTGGAACGATAACAAAGCCTCTT TGATCGAATATATAAAACAGGTGCACCTAGGGGTAAAGGGTCAAGTGTTCGATCAGAGTGGAGCTCCATT ACCGAATGTAATCGTGGAAGTCCAAGACAGAAAGCATATCTGCCCGTTTAGAACCAACAAGCTTGGAGAA TACTATCTGCTTCTGCTGCCCGGGTCCTACGTGATCAATGTTACAGTCCCTGGACACGACTCCTACCTCA CGAAGCTTACTATTCCAGGGAAATCCCAGCCCTTCAGTGCTCTTAAAAAGGATTTTCACCTCCCGCTGCG ATGGCAGCCGGATTCCATCTCCGTATCCAATCCTTCGTGCCCGATGATTCCGCTGTACAAATTCATGCCA AGCCACTCGGCTGCCACAAAGCCTAGTCTGGGCGTGTTTTTCATGACTCTTTTGTACGTATTTTTTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207070 representing BC100404 Red=Cloning site Green=Tags(s) MDRARLWLGLLLPVVAALDFRYHHQEGMEAFLKSVAQNYSSITHLHSIGKSVRGRNLWVLVVGQTPKEHR VGIPEFKYVANMHGDETVGRELLLHLIDYLVSSYRKDPEITHLIDSTRIHIMPSMNPDGFEAVQKPDCYY SNGRENYNNYDLNRNFPDAFENNNVTKQPETLAIMEWLKTETFVLSANLHGGALVASYPFDNGVQATGTL LSRSLTPDDDVFQHLAYTYASRNPNMTKGDQCKNKRNFPNGIINGYSWYPLQGGMQDYNYIWAQCFEITL ELSCCKYPREEKLPLFWNDNKASLIEYIKQVHLGVKGQVFDQSGAPLPNVIVEVQDRKHICPFRTNKLGE YYLLLLPGSYVINVTVPGHDSYLTKLTIPGKSQPFSALKKDFHLPLRWQPDSISVSNPSCPMIPLYKFMP SHSAATKPSLGVFFMTLLYVFFK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC100404
ORF Size	1331 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC100404, AAI00405
RefSeq Size	3155 bp
RefSeq ORF	1331 bp
Locus ID	70574
Gene Summary	Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins (By similarity).[UniProtKB/Swiss-Prot Function]

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