Pacsin1 (NM_178365) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A830061D09Rik; H74; mKIAA1379; syndapin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207030 representing NM_178365 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCTCCTACGATGAGGCCTCAGAGGAGATCACAGATAGCTTCTGGGAGGTGGGGAACTACAAGC GGACGGTGAAGCGCATCGACGATGGGCACCGCCTGTGCAACGACCTCATGAGCTGCGTGCAGGAGCGCGC CAAGATCGAGAAGGCATACGCGCAGCAGCTCACCGACTGGGCCAAGCGCTGGCGCCAGCTCATCGAGAAA GGTCCTCAGTATGGCAGCCTGGAGCGGGCGTGGGGCGCCATGATGACAGAAGCAGATAAGGTCAGCGAGC TGCACCAGGAGGTGAAGAACAGCCTGCTGAATGAGGACCTGGAGAAAGTCAAGAACTGGCAGAAGGATGC CTATCACAAGCAGATCATGGGTGGCTTCAAGGAGACGAAAGAGGCCGAGGATGGCTTCCGAAAGGCCCAG AAGCCCTGGGCTAAAAAGATGAAGGAGCTAGAGGCGGCCAAGAAGGCCTATCACTTGGCTTGTAAGGAGG AAAGGCTGGCCATGACCCGGGAGATGAACAGTAAGACAGAGCAGTCGGTCACCCCTGAACAGCAGAAGAA ACTTGTGGACAAAGTGGACAAATGCAGACAGGATGTGCAAAAGACTCAGGAGAAGTATGAGAAGGTGCTG GAAGATGTGGGCAAGACCACACCACAGTACATGGAGGGCATGGAGCAGGTGTTTGAGCAGTGCCAGCAGT TTGAGGAGAAGCGGCTGGTCTTCCTGAAGGAAGTCCTGCTGGATATCAAACGGCATCTCAACCTAGCGGA GAACAGCAGCTACATGCATGTCTACCGAGAACTGGAGCAGGCCATCCGGGGGGCCGATGCCCAGGAGGAC CTCAGGTGGTTCCGCAGCACCAGTGGCCCCGGGATGCCCATGAACTGGCCGCAGTTCGAGGAGTGGAACC CAGACCTCCCGCACACCACTGCCAAGAAGGAGAAACAGCCTAAGAAGGCAGAGGGGGCCACCCTGAGCAA TGCCACTGGGGCTGTAGAATCCACATCCCAGGCTGGGGACCGTGGCAGTGTTAGCAGCTATGACCGAGGC CAAACATATGCCACCGAGTGGTCAGACGATGAGAGCGGAAACCCCTTCGGGGGCAATGAGGCCAATGGTG GCGCCAACCCCTTCGAGGATGATGCCAAGGGAGTTCGTGTACGGGCACTCTATGACTACGACGGTCAGGA GCAGGATGAGCTCAGCTTCAAGGCCGGAGATGAGCTCACCAAGCTCGGAGAGGAAGACGAACAGGGTTGG TGCCGCGGGCGGCTGGACAGCGGACAGCTGGGCCTCTATCCTGCCAACTACGTTGAGGCTATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207030 representing NM_178365 Red=Cloning site Green=Tags(s) MSGSYDEASEEITDSFWEVGNYKRTVKRIDDGHRLCNDLMSCVQERAKIEKAYAQQLTDWAKRWRQLIEK GPQYGSLERAWGAMMTEADKVSELHQEVKNSLLNEDLEKVKNWQKDAYHKQIMGGFKETKEAEDGFRKAQ KPWAKKMKELEAAKKAYHLACKEERLAMTREMNSKTEQSVTPEQQKKLVDKVDKCRQDVQKTQEKYEKVL EDVGKTTPQYMEGMEQVFEQCQQFEEKRLVFLKEVLLDIKRHLNLAENSSYMHVYRELEQAIRGADAQED LRWFRSTSGPGMPMNWPQFEEWNPDLPHTTAKKEKQPKKAEGATLSNATGAVESTSQAGDRGSVSSYDRG QTYATEWSDDESGNPFGGNEANGGANPFEDDAKGVRVRALYDYDGQEQDELSFKAGDELTKLGEEDEQGW CRGRLDSGQLGLYPANYVEAI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_178365
ORF Size	4230 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_178365.4
RefSeq Size	1745 bp
RefSeq ORF	1326 bp
Locus ID	23969
UniProt ID	Q61644
Gene Summary	Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission.[UniProtKB/Swiss-Prot Function]

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