Sptlc3 (BC094496) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	RP23-347C2.1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206971 representing BC094496 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAATCTGAACGACAGTGCTGTTACAAATGGGACTCTACACAATCCTAAGACCCAGCAAGGAAAAA GGCAAAGCACTGGTTGTGTGAAAAATGGAATCTCAAAGGAAGCCCAGCAAAATAGGAAGGCATATGCTGA GGATAAGCCAGTCTTTGAGCCCTACCAGGAAGCACCACTTTATGTCTACGTTTTAACTTACATGGGATAT GGAATTGGAATCCTATTCGGCTATCTCAGAGACTTTATGAGAAACTGGGGAATTGAAAAATGCAATGCAG CTGTAGAGCGAGAAGAACAAAAAGACTTTGTGCCGCTGTATCAGGACTTTGAGAACTTTTATAAGAGGAA CCTTTACATGAGAATCAGAGACAGCTGGAGTCACACGGTCTGCAGTGCCCCAGAGCCTTACATGAATGTC ATGGAGAAAGTGACTGATGACTATAACTGGACATTCAGGCATACTGGAAAAGTTATAGAAAATATCATCA ACATGGCCTCCTACAACTACCTTGGGCTTGCAGGGAAATATGATGATTCGATGGTGAGAGTTAAGGATAC TTTAGAAAAGTATGGTGTAGGTGTGGCCAGCACCAGAAATGAAATGGGTACCTTGGATATACACAAGGAG TTGGAGGATCTCATGGCTGAGTTCTTGAATGTAGAAGCAGTGATGTCTTTTGGAATGGGATTCGCAACCA ACGCAATGAATATCCCTGTATTTGTTGGAAAGGGATGCCTCATTTTAAGTGATGAGTTTAACCACACTTC AGTCATCCTAGGATCTCGACTCTCAGGTGCAGTCATAAGACCCTTCAAACACAATAATGCAGAAAACCTA GAGAAGCTCCTGAGAGAAGCTATCATCCGTGGCCAGCCTGGCACAGGGAGAGCTTGGAAAAAGATTCTCA TCGTGGTAGAGGGCGTCTACAGCATGGAAGGTTCCATTGTGAACCTTGCCCAGATAGTGGCTCTAAAGAA GAAATACAAGGCTTACCTGTACATTGATGAAGCTCACAGTATTGGCTGTACAGGCCCCACGGGACGAGGC GTCAGAGAGTTATTTGGACTGGACCCTGAAGATATTGACGTATACATGGGTACATTCACCAAGAGCTTCT CGGGCTCAGGAGGATATATAGGCGGGAAAAAGGAAATTGTGGATTATTTACGGATGCAGTCACATAGCAC TACTTATGCTACATCCATGAGTCCCGTAGTAGCAGCACAACTCATCAGATCATTGAAGATAACTATGGAT ATGAGGGGAACATTGGAGGTATGGAGAGAATACAGCAACTTAAGGAAAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206971 representing BC094496 Red=Cloning site Green=Tags(s) MANLNDSAVTNGTLHNPKTQQGKRQSTGCVKNGISKEAQQNRKAYAEDKPVFEPYQEAPLYVYVLTYMGY GIGILFGYLRDFMRNWGIEKCNAAVEREEQKDFVPLYQDFENFYKRNLYMRIRDSWSHTVCSAPEPYMNV MEKVTDDYNWTFRHTGKVIENIINMASYNYLGLAGKYDDSMVRVKDTLEKYGVGVASTRNEMGTLDIHKE LEDLMAEFLNVEAVMSFGMGFATNAMNIPVFVGKGCLILSDEFNHTSVILGSRLSGAVIRPFKHNNAENL EKLLREAIIRGQPGTGRAWKKILIVVEGVYSMEGSIVNLAQIVALKKKYKAYLYIDEAHSIGCTGPTGRG VRELFGLDPEDIDVYMGTFTKSFSGSGGYIGGKKEIVDYLRMQSHSTTYATSMSPVVAAQLIRSLKITMD MRGTLEVWREYSNLRKT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC094496
ORF Size	1313 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC094496, AAH94496
RefSeq Size	1998 bp
RefSeq ORF	1313 bp
Locus ID	228677
Gene Summary	Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. SPT complexes containing SPTLC3 generate shorter chain sphingoid bases compared to complexes containing SPTLC2. The SPTLC1-SPTLC3-SPTSSA isozyme uses C12-CoA, C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. On the other hand, the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.