Vps4a (NM_126165) Mouse Tagged ORF Clone
CAT#: MG206965
- TrueORF®
Vps4a (tGFP-tagged) - Mouse vacuolar protein sorting 4a (yeast) (Vps4a)
ORF Plasmid: DDK
"NM_126165" in other vectors (3)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 4930589C15Rik; AI325971; AW553189 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206965 representing NM_126165
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACGTCAACCCTCCAGAAAGCCATCGATCTGGTGACAAAAGCCACAGAAGAGGATAAAGCCAAGA ACTACGAGGAGGCACTCCGTCTCTACCAGCATGCTGTGGAGTATTTCCTCCACGCCATCAAATATGAAGC ACACAGTGACAAGGCCAAGGAGAGCATTCGAGCAAAGTGCATGCAGTACCTAGACAGAGCGGAGAAGCTG AAGGATTATTTACGAAACAAAGAGAAACATGGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAAGGCAAGG GTAGTGACAGTGACAGTGAAGGGGATAATCCAGAGAAAAAGAAGTTACAAGAACAATTGATGGGTGCTGT TGTGATGGAGAAGCCGAACATACGGTGGAATGATGTAGCTGGACTGGAGGGGGCTAAAGAGGCTCTCAAA GAAGCTGTGATTTTGCCAATTAAATTCCCACACTTATTCACAGGCAAACGCACTCCTTGGCGAGGCATAC TCCTCTTTGGGCCCCCAGGCACAGGCAAGTCCTACCTGGCCAAAGCCGTGGCAACAGAAGCCAACAACTC TACCTTCTTCTCTGTGTCCTCCTCGGATCTGATGTCTAAGTGGTTGGGGGAAAGCGAGAAGCTAGTCAAG AACCTGTTTGAGCTGGCCCGGCAGCATAAGCCTTCCATCATCTTCATTGATGAGGTAGATTCCCTCTGTG GGTCACGGAATGAAAATGAGAGTGAAGCCGCTCGTAGGATCAAAACAGAGTTCTTGGTCCAGATGCAAGG AGTGGGGAATAATAATGATGGAACTCTTGTTCTTGGTGCCACAAACATCCCCTGGGTGTTGGATTCAGCC ATCAGGAGGAGGTTTGAAAAGCGAATTTATATCCCATTGCCAGAGGAAGCGGCCCGTGCCCAAATGTTTC GGTTACATCTGGGAAGCACACCTCACAACCTCACAGATGCTAACATTCACGAGCTGGCCCGCAAGACAGA AGGCTACTCAGGAGCAGATATCAGCATCATTGTGCGGGACTCCCTCATGCAGCCAGTCAGAAAAGTACAG TCAGCAACACACTTCAAGAAGGTGTGTGGCCCTTCTCGAACCAACCCTAGCGTTATGATTGATGATCTTC TGACCCCATGTTCTCCGGGAGATCCAGGGGCCATAGAGATGACTTGGATGGATGTCCCTGGTGATAAACT CTTAGAGCCTGTGGTTTGCATGTCGGACATGCTCCGGTCTTTGGCCACTACCCGGCCCACAGTGAATGCA GACGACCTCCTGAAAGTAAAGAAATTCTCTGAGGACTTTGGACAGGAGAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206965 representing NM_126165
Red=Cloning site Green=Tags(s) MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKL KDYLRNKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALK EAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVK NLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSA IRRRFEKRIYIPLPEEAARAQMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQ SATHFKKVCGPSRTNPSVMIDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNA DDLLKVKKFSEDFGQES TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_126165 |
ORF Size | 1311 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_126165.1, NP_569053.1 |
RefSeq Size | 2089 bp |
RefSeq ORF | 1314 bp |
Locus ID | 116733 |
UniProt ID | Q8VEJ9 |
Gene Summary | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR206965 | Vps4a (Myc-DDK-tagged) - Mouse vacuolar protein sorting 4a (yeast) (Vps4a) |
CNY 3,656.00 |
|
MR206965L3 | Lenti ORF clone of Vps4a (Myc-DDK-tagged) - Mouse vacuolar protein sorting 4a (yeast) (Vps4a) |
CNY 5,230.00 |
|
MR206965L4 | Lenti ORF clone of Vps4a (mGFP-tagged) - Mouse vacuolar protein sorting 4a (yeast) (Vps4a) |
CNY 5,230.00 |