Trnt1 (BC031764) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mt-Trat, CGI-47, 9830143O18Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206916 representing BC031764 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGCGTCCTGTATCCTTGGCACAGGCAGGTCCTGAGGTGTAGTTGGAGTAGATTGTGCCTTCTGA AGCGATACCTGTTTACAATGAAGTTGCAGTCCCCAGAATTCCAGTCCCTTTTCACCGAAGGATTGAAGAG CCTGACAGAATTATTTGCCAAAGAGAATCATGAATTAAGAATAGCAGGAGGAGCAGTGAGGGATTTACTG AATGGGGTGAAGCCTCAAGATGTGGATTTCGCCACCACTGCCACCCCTACTCAGATGAAGGAGATGTTCC AGTCAGCTGGCATTCGCATGATCAACAACAAAGGAGAGAAACATGGGACTATCACTGCCAGGCTTCATGA AGAAAATTTTGAAGTTACTACACTCCGAATTGATGTTACCACTGATGGAAGACATGCAGAGGTAGAATTC ACAACTGACTGGCAGAAAGACGCTGAACGAAGAGATCTCACTATAAATTCTATGTTTCTAGGTTTTGATG GTACACTGTTTGATTACTTTAATGGTTATGCAGATTTAAAAAATAAAAAGGTTCGATTTGTTGGACATGC AAAACAGAGAATTCAGGAAGATTATCTTCGAATTTTAAGGTATTTCAGGTTTTATGGCAGAATTGTCGAC AGACCTGGTGACCATGACCATGAGACTCTAGAAGCAATTGCAGAAAATGCCAAAGGTTTGGCTGGAATAT CAGGAGAGAGGATTTGGGTAGAACTGAAGAAAATTCTTACTGGTGACCATGTAAACCATTTGATCCACCT GATCTATGATCTTGGTGTGGCTCCTCACATAGGTTTACCTGCTAATGCAAATTTAGAAGAATTTAACAAA GTCAGTAAAAATGTTGAAGGCTTTTCACCAAAACCAATGACTCTTTTGGCTTCTTTATTCAAAGTACAAG ATGATGTCACAAAATTGGACTTGAGATTGAAAATTTCAAAAGAGGAGAAAAACCTGGGTTTATTTATAGT TAAAAACAGGAAAGACTTGATTAAAGCAACAGATAGTTCAGAACCATTGAAACCATACCAAGACTTTGTG ATAGACTCTAGGGAACCAGATGCAACTGCCCGTGTTTGTGAGCTGCTGAAGTACCAAGGAGAGCATGGTC TTCTCAAAGAGATGCAGCAGTGGTCTGTCCCGCCGTTCCCTGTGAGTGGACATGACATCAGGAAAGTGGG TATTTCTTCAGGGAAGGAAATTGGGGCCCTGCTACAGCAGTTGCGTGAACAGTGGAAGAAAAGTGGTTAC CGGATGGAAAAAGATGAACTACTAAGTTACATAAAGAAGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206916 representing BC031764 Red=Cloning site Green=Tags(s) MQSVLYPWHRQVLRCSWSRLCLLKRYLFTMKLQSPEFQSLFTEGLKSLTELFAKENHELRIAGGAVRDLL NGVKPQDVDFATTATPTQMKEMFQSAGIRMINNKGEKHGTITARLHEENFEVTTLRIDVTTDGRHAEVEF TTDWQKDAERRDLTINSMFLGFDGTLFDYFNGYADLKNKKVRFVGHAKQRIQEDYLRILRYFRFYGRIVD RPGDHDHETLEAIAENAKGLAGISGERIWVELKKILTGDHVNHLIHLIYDLGVAPHIGLPANANLEEFNK VSKNVEGFSPKPMTLLASLFKVQDDVTKLDLRLKISKEEKNLGLFIVKNRKDLIKATDSSEPLKPYQDFV IDSREPDATARVCELLKYQGEHGLLKEMQQWSVPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGY RMEKDELLSYIKKT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC031764
ORF Size	1304 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC031764, AAH31764
RefSeq Size	2240 bp
RefSeq ORF	1304 bp
Locus ID	70047
Gene Summary	Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates.[UniProtKB/Swiss-Prot Function]

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