Arid5a (BC027152) Mouse Tagged ORF Clone

CAT#: MG206872

  • TrueORF®

Arid5a (tGFP-tagged) - Mouse AT rich interactive domain 5A (Mrf1 like) (cDNA clone MGC:36895 IMAGE:4935168)

ORF Plasmid: DDK tGFP



  "BC027152" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,760.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC36895, Mrf1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206872 representing BC027152
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGGAGCTGAGGGGAGACGATGGGACCACTGAGAAGCTGAAGAAGGCCAAGGACTCAGAGGAGA
GGCGGGTGGAGCAGACCACGCCAGGAAAGACCAAATCAGATGCCACTGGCCAGACACAGCTTCCCTGCCA
GGGATCCTCGAGGGACAGCACAGAACAGCTGGGCCCAGTATCTGGACCCTCTCCACCACTCACGGGTGCT
AGTAGCTGCCCTGAGGCCTACAAGCGGCTCTTGTCAAGCTTTTACTGCAAAGGGGCGCATGGCATCATGT
CACCACTGGCCAAAAAGAAACTCCTGGCCCAGGTCAGCAAGGCAGAGGCCTTGCAGTGCCAAGAAGAGGG
CTGTCGCCATGGAGCAAGGAGCCCCAACAAGGACATTCAAGACAGTCCCCAGAACCTAAGAGGGCCGGCT
GAGAACTCTGAACACCAGCTAACCCCCCGGGAAGGATTGCAGGCCCCTGGTGGGAGCACCAGGATGGAGG
CCCAAGTGGGCCCCTGCCCTACAGCCCCCATGTTCTCAGGCTGTTTTCATGCGTACCCCACCGAGGTGCT
GAAACCTGTCAGCCAGCACCCTAGGGACTTCTTCTCCGGCCTTAAAGACAGGGTGCTGTTGGGACCACCT
GGTAAAGAAGAAGGTCCGACAACCAAAGAGTCCCATCTGGTGTGGGGTGGGGATGCCAACCACCCCTCTG
CATTCCATAAAGGCAGCACAAGAAAAAGAAGTTTCTACCCCAAACCCAAAGCCTGCTGGGTGTCTCCCAT
GGCCAAGGTCCCTACTGAGAGGCCTGGAGCCCCATCCCCTCATCCCAGTAGCCCAGGTCTTGGCAGTAAG
CGCGGCTTGGAAGAAGAGGGATTCGCTCATGGTGGCAAGAAACTGAGGGCAGTGTCTCCCTTTCTGAAGG
AGGTGGATTCCAAGGAGACTGGGGGCAAGCCTGCAGCCCCTGGCTTGGCTGTATCCTGTCTACTGGGCCC
AACCCCGGGGCCCACTCCTCCAGAGGCCTACAGGGGCACCATGCTGCGGTGTCCTCTAAACTTCACCGGT
AGCGCAGACCCTCTGAAGGGCCAGGCCTCACTCCCCTTCAGCCCCCTGGTCATCCCTGCTTTCCCAGCCC
ACCTTCTGGCTACAACAGGCCCCTCACCTATGGCTGCCAGCCTGATGCATTTCCCTCCCACGCCCTATGA
CGCTGTCCTACGCAACAGACTGGGTCCAGCTTCGTCTGCCTGGCACATGCCACCCGTCACAACCTATGCG
GCACCTCACTTCTTCCACCTCAACACCAAACTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206872 representing BC027152
Red=Cloning site Green=Tags(s)

MAKELRGDDGTTEKLKKAKDSEERRVEQTTPGKTKSDATGQTQLPCQGSSRDSTEQLGPVSGPSPPLTGA
SSCPEAYKRLLSSFYCKGAHGIMSPLAKKKLLAQVSKAEALQCQEEGCRHGARSPNKDIQDSPQNLRGPA
ENSEHQLTPREGLQAPGGSTRMEAQVGPCPTAPMFSGCFHAYPTEVLKPVSQHPRDFFSGLKDRVLLGPP
GKEEGPTTKESHLVWGGDANHPSAFHKGSTRKRSFYPKPKACWVSPMAKVPTERPGAPSPHPSSPGLGSK
RGLEEEGFAHGGKKLRAVSPFLKEVDSKETGGKPAAPGLAVSCLLGPTPGPTPPEAYRGTMLRCPLNFTG
SADPLKGQASLPFSPLVIPAFPAHLLATTGPSPMAASLMHFPPTPYDAVLRNRLGPASSAWHMPPVTTYA
APHFFHLNTKL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC027152
ORF Size 1295 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC027152, AAH27152
RefSeq Size 2055 bp
RefSeq ORF 1295 bp
Locus ID 214855
Gene Summary DNA-binding protein that may regulate transcription and act as a repressor by binding to AT-rich stretches in the promoter region of target genes (By similarity). May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (PubMed:21346191). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (By similarity). As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3' UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells (PubMed:23676272, PubMed:27022145). In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling (PubMed:24782182). Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (PubMed:27671645).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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