Psmd11 (BC090980) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	S9, P44.5
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206719 representing BC090980 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCAGCGGTGGTGGAGTTCCAGAGAGCCCAGTCTCTACTCAGCACCGACCGGGAGGCCTCCA TCGACATCCTCCACTCCATCGTGAAACGTGACATTCAAGAAAATGATGAGGAGGCAGTCCAGGTCAAAGA GCAGAGCATCCTTGAACTGGGTTCTCTCCTGGCGAAGACTGGACAAGCTGCTGAGCTTGGAGGACTCCTG AAGTATGTACGGCCTTTCTTGAATTCCATCAGTAAAGCTAAAGCAGCTCGTCTGGTCCGGTCTCTTCTTG ATCTGTTTCTAGATATGGAAGCAGCCACAGGGCAGGAGGTCGAGCTATGTTTAGAGTGCATCGAATGGGC CAAATCAGAGAAAAGAACTTTCTTACGCCAAGCATTGGAGGCAAGGCTGGTGTCTTTGTATTTTGATACC AAGAGGTACCAGGAAGCATTGCATTTGGGTTCTCAGCTGCTTCGGGAGTTGAAAAAGATGGATGATAAAG CCCTTTTGGTGGAAGTACAGCTTTTAGAAAGCAAAACTTACCATGCTCTGAGTAATCTGCCGAAAGCCCG AGCTGCCTTAACCTCTGCTCGAACCACAGCAAATGCCATCTACTGCCCCCCTAAATTGCAGGCCACCCTG GACATGCAGTCAGGCATTATTCATGCAGCAGAGGAGAAGGACTGGAAAACTGCATACTCATACTTCTATG AGGCATTCGAAGGCTACGACTCCATTGATAGCCCCAAGGCCATCACATCTCTGAAGTACATGTTGCTGTG CAAAATCATGCTCAACACCCCAGAAGATGTCCAGGCTTTGGTGAGCGGAAAGCTTGCACTTCGGTATGCA GGGAGGCAGACAGAAGCATTGAAATGTGTGGCTCAAGCTAGCAAGAACAGATCACTGGCAGATTTTGAAA AGGCCCTGACAGACTACAGGGCAGAGCTCCGGGATGACCCAATCATCAGCACACATTTGGCCAAGCTGTA CGATAACTTACTGGAACAGAATCTGATCCGGGTCATCGAGCCTTTTTCCCGAGTCCAGATTGAACACATA TCTAGCCTCATCAAACTCTCCAAGGCCGACGTGGAAAGAAAATTATCACAGATGATTCTTGACAAGAAGT TTCATGGGATTTTGGACCAGGGGGAGGGTGTCCTGATCATTTTCGATGAACCCCCAGTAGATAAAACTTA TGAAGCTGCTCTGGAAACTATTCAGAACATGAGTAAAGTAGTGGACTCCCTCTACAGCAAAGCCAAGAAG CTGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206719 representing BC090980 Red=Cloning site Green=Tags(s) MAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLL KYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDT KRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATL DMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYA GRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHI SSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYSKAKK LT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC090980
ORF Size	1268 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC090980, AAH90980
RefSeq Size	1572 bp
RefSeq ORF	1268 bp
Locus ID	69077
Gene Summary	Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.[UniProtKB/Swiss-Prot Function]

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