Ap3m1 (BC090983) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1200013D09Rik; C78982; R75378
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206620 representing BC090983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCACAGTCTATTTCTCATAAACTGCTCTGGCGACATTTTTCTAGAAAAACACTGGAAGAGCGTTG TAAGCCAGTCAGTGTGTGACTATTTCTTTGAAGCTCAGGAGAAAGCTGCTGATGTTGAAAATGTCCCACC TGTCATTTCAACACCTCACCACTACCTCATTAGTATCTACCGGGATAAGCTCTTCTTTGTGTCTGTGATA CAGACCGAAGTGCCACCTCTCTTTGTAATTGAATTTCTGCATCGAGTTGCTGACACTTTTCAGGACTACT TTGGTGAGTGTTCAGAGGCTGCAATTAAGGATAATGTGGTCATAGTATATGAGCTCTTGGAAGAAATGTT AGACAATGGATTCCCACTGGCTACTGAATCTAACATTTTGAAAGAACTGATTAAACCACCAACAATTCTA CGTTCTGTCGTCAATTCTATTACAGGCAGTAGTAATGTTGGGGATACACTCCCCACTGGGCAGCTATCCA ACATCCCATGGCGTCGAGCAGGAGTGAAGTACACAAACAATGAAGCCTATTTTGATGTAGTTGAAGAGAT AGATGCAATTATAGATAAATCAGGATCTACAGTCTTTGCAGAAATTCAAGGGGTCATTGATGCTTGCATT AAGCTGTCTGGAATGCCTGATCTCTCCCTCTCTTTCATGAACCCAAGGCTACTAGATGACGTCAGCTTCC ACCCATGCATCCGATTCAAACGCTGGGAGTCTGAAAGAGTTTTGTCATTCATTCCTCCAGATGGAAATTT CCGACTCATATCGTATCGTGTCAGCTCACAAAATCTAGTGGCAATACCAGTGTATGTGAAACACAGTATC AGCTTTAAGGAAAACAGCTCTTGTGGCCGGTTTGATATAACAATTGGACCGAAACAGAATATGGGAAAAA CAATTGAAGGAATCACAGTGACTGTTCACATGCCCAAAGTTGTGCTGAATATGAACCTGACACCAACGCA AGGCAGCTATACATTTGATCCCGTGACCAAGGTACTAGCATGGGATGTGGGCAAAATTACTCCACAAAAG CTCCCAAGTCTTAAAGGACTAGTAAATTTACAGTCAGGAGCACCCAAGCCAGAAGAGAACCCAAACCTCA ACATACAGTTCAAGATCCAGCAGCTTGCTATTTCAGGCTTAAAAGTGAACCGCTTGGACATGTATGGTGA GAAGTATAAGCCATTTAAAGGAGTCAAATATGTCACAAAGGCTGGGAAGTTCCAAGTGAGGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206620 representing BC090983 Red=Cloning site Green=Tags(s) MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVI QTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTIL RSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACI KLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSI SFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLAWDVGKITPQK LPSLKGLVNLQSGAPKPEENPNLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC090983
ORF Size	1256 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC090983, AAH90983
RefSeq Size	2186 bp
RefSeq ORF	1256 bp
Locus ID	55946
Gene Summary	Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function]

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