Mbd2 (NM_010773) Mouse Tagged ORF Clone
CAT#: MG206536
- TrueORF®
Mbd2 (tGFP-tagged) - Mouse methyl-CpG binding domain protein 2 (Mbd2). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_010773" in other vectors (2)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MBD2a |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206536 representing NM_010773, codon optimized.
Due to the complexity of NM_010773, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGCCCACCCAGGAGGGGGAAGGTGTTGCCCCGAACAGGAAGAGGGCGAGAGTGCTGCTGGAGGCA GTGGCGCCGGTGGAGACAGCGCAATCGAGCAGGGAGGACAGGGTTCTGCATTGGCACCGAGCCCGGTGAG CGGAGTGAGAAGAGAGGGTGCACGAGGCGGGGGACGCGGCAGAGGCCGGTGGAAACAGGCGGCTCGGGGC GGTGGGGTCTGCGGCAGGGGACGCGGGAGAGGCAGAGGCCGGGGGCGCGGCCGCGGCAGGGGACGAGGCA GGGGCAGACCTCAGAGTGGCGGGAGCGGCCTTGGAGGTGACGGCGGAGGTGGCGCAGGTGGCTGCGGCGG GGGAAGCGGAGGAGGAGTCGCCCCTCGCAGAGATCCCGTACCGTTTCCTAGCGGCAGTTCAGGTCCAGGA CCACGCGGGCCAAGAGCCACCGAAAGCGGGAAGCGGATGGACTGTCCCGCCCTTCCTCCAGGCTGGAAAA AGGAAGAGGTGATCAGGAAATCTGGACTCTCCGCTGGAAAATCTGATGTGTACTACTTCAGTCCTTCTGG TAAGAAGTTCCGGTCCAAACCTCAGCTGGCCCGCTATCTGGGCAATGCTGTCGATCTCTCTTCATTTGAT TTCCGGACTGGTAAGATGATGCCGTCAAAGCTTCAGAAGAATAAGCAGAGGCTGAGAAACGACCCTCTGA ACCAGAATAAGGGGAAGCCCGATCTTAATACGACCCTCCCGATTCGCCAGACAGCGAGTATCTTTAAGCA GCCAGTGACCAAGTTTACTAATCATCCATCCAATAAAGTGAAGTCTGACCCTCAGCGCATGAACGAGCAG CCCCGACAGCTTTTCTGGGAGAAGAGACTCCAGGGACTCAGCGCTTCAGACGTTACTGAACAGATCATCA AAACCATGGAACTCCCCAAAGGACTGCAGGGCGTTGGACCAGGTAGCAATGATGAAACGCTGCTGTCTGC GGTGGCGAGCGCGCTCCACACATCTAGTGCCCCAATCACCGGGCAGGTGTCCGCAGCCGTGGAGAAGAAC CCCGCTGTATGGCTCAATACTAGTCAGCCGCTGTGTAAGGCATTCATCGTGACAGATGAAGATATCAGAA AACAAGAGGAGAGGGTTCAGCAGGTGCGCAAAAAGCTGGAAGAAGCTCTGATGGCTGACATATTGTCACG CGCTGCTGACACTGAAGAAGTCGACATCGATATGGACAGTGGGGACGAAGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206536 representing NM_010773
Red=Cloning site Green=Tags(s) MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAARG GGVCGRGRGRGRGRGRGRGRGRGRGRPQSGGSGLGGDGGGGAGGCGGGSGGGVAPRRDPVPFPSGSSGPG PRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNAVDLSSFD FRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKFTNHPSNKVKSDPQRMNEQ PRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKN PAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEVDIDMDSGDEA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010773 |
ORF Size | 1242 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010773.2, NP_034903.2 |
RefSeq Size | 1866 bp |
RefSeq ORF | 1245 bp |
Locus ID | 17191 |
UniProt ID | Q9Z2E1 |
Gene Summary | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (By similarity). May enhance the activation of some unmethylated cAMP-responsive promoters (By similarity). Selectively represses transcription activity of methylated rRNA promoters.[UniProtKB/Swiss-Prot Function] |
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SDS |