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Hyal3 (NM_178020) Mouse Tagged ORF Clone
CAT＃: MG206489
- TrueORF®
Hyal3 (tGFP-tagged) - Mouse hyaluronoglucosaminidase 3 (Hyal3)
ORF Plasmid: DDK
"NM_178020" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Hyl3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206489 representing NM_178020
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCATGCACCTAGGCCTAATGATGGTGGTGGGGTTAACCCTGTGCCTGATGCATGGCCAAGCTTTGC TGCAGGTTCCTGAACATCCTTTTTCTGTGGTATGGAATGTACCCTCAGCAAGATGTAAGGCCCATTTTGG TGTGCATCTGCCTCTCGATGCCCTCGGCATTGTAGCCAACCACGGCCAACATTTTCACGGCCAAAACATC TCCATCTTCTACAAGAACCAGTTTGGTCTTTATCCTTACTTTGGACCTAGAGGTACAGCCCACAATGGGG GAATCCCTCAGGCTGTGTCCTTAGACCACCACTTGGCACGAGCTGCCCACCAGATCCTACACAGCCTAGG ATCTAGCTTTGCTGGCTTGGCAGTGCTGGACTGGGAAGAATGGTACCCACTCTGGGCTGGGAACTGGGGC CCCCATCGACAAGTCTACCTGGCAGCCTCCTGGGTTTGGACACAGCAGATGTTCCCTGGCTTGGATCCTC AGGAACAGCTCCACAAAGCCCATACTAGCTTTGAGCAGGCTGCCCGTGCACTCATGGAATACACTCTGCA GCTGGGCCGGACACTTCGCCCGAGTGGCCTCTGGGGCTTTTACAGATATCCAGCCTGTGGCAATGGCTGG CATAAGATGGCTTCCAACTACACAGGCCACTGCCATGCAGCCATCACCACCCAAAACACCCAACTGCGTT GGCTCTGGGCTGCCTCCAGTGCTCTCTTCCCTAGCATCTACCTCCCACCCAGACTGCCACTTGCCTACCG TCAGGCCTTTGTCCGACACCGCCTGGAGGAAGCCTTCCGTGTAGCCCTTTTGGAGCATTCACATCCTCTA CCTGTTCTGGCTTATTCTCGCCTCACACACCGGAGCTCTGGGAGATTCCTGTCTCTGGACGACCTGATGC AGACTATTGGAGTGAGTGCCGCACTGGGAACAGCTGGAGTGGTACTCTGGGGGGACCTGAGCTTCTCTAG CTCTGAGGAAAAGTGCTGGCGTCTCCATGACTACTTAGTGGGCACTTTAGGCCCCTATGTGATCAATGTG ACCAAGGCTGACATGGCTTGCAGTCACCAGCGATGTCATGGCCATGGTCGATGTGCCCGGAAAGACCCAG GACAAATGGAAGCCTTTCTACATCTGCAGCCAGATGACAGTCTTGGAGCTTGGAATTCCTTCAGATGCCA TTGTTATTCGGGTTGGGCTGGCCCTACCTGCCTGGAGCCTAAACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206489 representing NM_178020
Red=Cloning site Green=Tags(s) MIMHLGLMMVVGLTLCLMHGQALLQVPEHPFSVVWNVPSARCKAHFGVHLPLDALGIVANHGQHFHGQNI SIFYKNQFGLYPYFGPRGTAHNGGIPQAVSLDHHLARAAHQILHSLGSSFAGLAVLDWEEWYPLWAGNWG PHRQVYLAASWVWTQQMFPGLDPQEQLHKAHTSFEQAARALMEYTLQLGRTLRPSGLWGFYRYPACGNGW HKMASNYTGHCHAAITTQNTQLRWLWAASSALFPSIYLPPRLPLAYRQAFVRHRLEEAFRVALLEHSHPL PVLAYSRLTHRSSGRFLSLDDLMQTIGVSAALGTAGVVLWGDLSFSSSEEKCWRLHDYLVGTLGPYVINV TKADMACSHQRCHGHGRCARKDPGQMEAFLHLQPDDSLGAWNSFRCHCYSGWAGPTCLEPKP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178020 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178020.2, NP_821139.1 |
| RefSeq Size | 1819 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 109685 |
| UniProt ID | Q8VEI3 |
| Gene Summary | Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm (PubMed:20586096). Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage (PubMed:18653706). Has no hyaluronidase activity in embryonic fibroblasts in vitro (PubMed:18234732). Has no hyaluronidase activity in granulosa cells in vitro (PubMed:18653706).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203870 | Hyal3 (untagged) - Mouse hyaluronoglucosaminidase 3 (Hyal3), (10ug) |
CNY 2,000.00 |
|
| MR206489 | Hyal3 (Myc-DDK-tagged) - Mouse hyaluronoglucosaminidase 3 (Hyal3) |
CNY 3,330.00 |
|
| MR206489L3 | Lenti ORF clone of Hyal3 (Myc-DDK-tagged) - Mouse hyaluronoglucosaminidase 3 (Hyal3) |
CNY 5,230.00 |
|
| MR206489L4 | Lenti ORF clone of Hyal3 (mGFP-tagged) - Mouse hyaluronoglucosaminidase 3 (Hyal3) |
CNY 5,230.00 |
