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Smarce1 (BC047141) Mouse Tagged ORF Clone
CAT＃: MG206466
- TrueORF®
Smarce1 (tGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (cDNA clone
ORF Plasmid: DDK
"BC047141" in other vectors (3)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810417B20Rik; 5830412H02Rik; 9030408N19Rik; Baf27 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206466 representing BC047141
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAAAAGACCATCTTATGCCCCACCTCCCACCCCAGCTCCTGCAACACAAATGCCCAGCACACCAG GGTTTGTGGGATACAATCCATACAGTCATCTCGCCTACAACAACTACAGGCTGGGAGGGAACCCGGGCAC CAACAGCCGGGTCACGGCGTCCTCTGGCATTACGATTCCAAAGCCTCCAAAGCCACCAGATAAGCCGCTG ATGCCCTACATGAGGTACAGCAGAAAGGTCTGGGACCAAGTAAAGGCTTCCAACCCTGACCTAAAGTTGT GGGAGATTGGCAAGATTATTGGTGGCATGTGGCGAGATCTCACTGATGAAGAGAAGCAAGAATATTTAAA CGAATACGAAGCAGAAAAGATAGAGTACAATGAGTCTATGAAGGCCTACCATAATTCCCCTGCGTACCTT GCCTATATTAATGCAAAAAGTCGTGCGGAAGCTGCATTAGAGGAAGAAAGTCGACAGAGACAGTCTCGCA TGGAGAAAGGAGAACCTTACATGAGCATTCAGCCTGCTGAGGATCCAGACGACTATGATGATGGCTTTTC AATGAAGCACACAGCCACTGCCCGTTTCCAGAGAAACCACCGTCTCATCAGTGAGATCCTCAGTGAGAGT GTGGTACCTGATGTGCGGTCGGTTGTCACAACAGCTAGAATGCAGGTCCTCAAGCGACAGGTCCAGTCTT TAATGGTTCATCAGCGGAAACTAGAAGCCGAGCTCCTTCAGATAGAGGAACGACACCAGGAAAAGAAGAG GAAATTCCTGGAAAGCACGGACTCCTTTAACAATGAACTTAAAAGGTTATGTGGTCTGAAGGTAGAAGTA GACATGGAGAAGATTGCGGCTGAGATCGCACAGGCGGAGGAACAGGCCCGCAAGAGGCAAGAAGAGAGGG AGAAGGAGGCAGCAGAGCAAGCTGAGCGCAGTCAGAGCAGCATGGCCCCTGAGGAAGAGCAAGTGGCGAA CAAAGCCGAGGAGAAGAAAGATGAGGAGAGCATCCCGATGGAGACAGAGGAGACACACCTTGAAGACACA GCAGAGAGCCAGCAGAATGGTGAAGAAGGCACGTCTACTCCTGAGGACAAGGAGAGTGGGCAGGAGGGGG TTGACAGCATGGAGGTGGAAGGGACCAGTGACAGTAACACGGGCTCAGAGAGCAACAGCGCCACAGTGGA GGAGCCGCCCACAGACCCAGTGCCAGAAGACGAGAAGAAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206466 representing BC047141
Red=Cloning site Green=Tags(s) MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPL MPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYL AYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSES VVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEV DMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSMAPEEEQVANKAEEKKDEESIPMETEETHLEDT AESQQNGEEGTSTPEDKESGQEGVDSMEVEGTSDSNTGSESNSATVEEPPTDPVPEDEKKE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC047141 |
| ORF Size | 1235 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC047141, AAH47141 |
| RefSeq Size | 2355 bp |
| RefSeq ORF | 1235 bp |
| Locus ID | 57376 |
| Gene Summary | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:12110891). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs)(PubMed:12145209).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206466 | Smarce1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (cDNA clone |
CNY 3,656.00 |
|
| MR206466L3 | Lenti ORF clone of Smarce1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (cDNA clone |
CNY 4,280.00 |
|
| MR206466L4 | Lenti ORF clone of Smarce1 (mGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (cDNA clone |
CNY 4,280.00 |
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