Lox (BC018439) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI893619; rrg; TSC-160
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206463 representing BC018439 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTTCGCCTGGGCTGTGCTCCTTCTGGGGCCACTGCAGCTTTGTCCCCTTCTCCGCTGCGCCCCGC AGACCCCGCGCGAGCCGCCCGCCGCCCCTGGTGCCTGGCGCCAGACAATCCAATGGGAGAACAACGGGCA GGTGTTCAGTCTGCTGAGCCTCGGGGCGCAGTACCAGCCTCAGCGACGCCGCGACCCCAGTGCCACTGCC CGGAGACCCGACGGCGACGCAGCCTCGCAGCCGCGCACGCCCATTCTTCTGCTGCGTGACAACCGCACCG CCTCTACCCGTGCGAGGACGCCAAGCCCGTCTGGGGTCGCCGCGGGTCGTCCCCGGCCCGCCGCCCGCCA CTGGTTCCAAGCTGGTTTCTCGCCGTCGGGGGCTCGCGATGGAGCCTCACGGCGCGCGGCGAACCGGACT GCATCGCCACAGCCTCCGCAGCTCAGTAATCTGAGGCCACCCAGCCACATAGATCGCATGGTGGGCGACG ACCCCTACAATCCCTACAAGTACTCCGACGACAACCCCTATTATAACTACTATGACACGTATGAGAGGCC CCGGCCCGGGAGCAGGAACCGACCTGGATACGGCACCGGTTACTTCCAGTACGGTCTCCCGGACCTGGTG CCCGACCCCTACTACATCCAGGCTTCCACGTACGTCCAGAAGATGTCTATGTACAACCTGAGATGCGCTG CGGAAGAAAACTGCCTGGCCAGTTCAGCATATAGGGCGGATGTCAGAGACTATGACCACAGGGTACTGCT ACGATTTCCGCAAAGAGTGAAGAACCAAGGGACATCGGACTTCTTACCAAGCCGCCCTCGGTACTCCTGG GAGTGGCACAGCTGTCACCAACATTACCACAGCATGGACGAATTCAGCCACTATGACCTGCTTGATGCCA ACACACAGAGGAGAGTGGCTGAAGGCCACAAAGCAAGCTTCTGTCTGGAGGACACGTCCTGTGACTATGG GTACCACAGGCGCTTTGCGTGCACTGCACACACACAGGGATTGAGTCCTGGATGTTATGACACCTATGCG GCAGACATAGACTGCCAGTGGATTGATATTACAGATGTACAACCTGGAAACTACATTCTAAAGGTCAGTG TAAACCCCAGCTACCTGGTGCCTGAATCAGACTACACTAACAATGTTGTACGCTGTGACATTCGCTACAC AGGACATCATGCCTATGCCTCAGGCTGCACAATTTCACCGTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206463 representing BC018439 Red=Cloning site Green=Tags(s) MRFAWAVLLLGPLQLCPLLRCAPQTPREPPAAPGAWRQTIQWENNGQVFSLLSLGAQYQPQRRRDPSATA RRPDGDAASQPRTPILLLRDNRTASTRARTPSPSGVAAGRPRPAARHWFQAGFSPSGARDGASRRAANRT ASPQPPQLSNLRPPSHIDRMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGSRNRPGYGTGYFQYGLPDLV PDPYYIQASTYVQKMSMYNLRCAAEENCLASSAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSW EWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYA ADIDCQWIDITDVQPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC018439
ORF Size	1233 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018439, AAH18439
RefSeq Size	3916 bp
RefSeq ORF	1235 bp
Locus ID	16948
Gene Summary	This gene encodes a precursor protein that belongs to the lysyl oxidase family of proteins. The secreted proprotein is proteolytically processed to an active mature peptide and a propeptide. This propeptide is thought to function in tumor suppression by inhibiting the Ras signaling pathway. The active enzyme plays a role in cross-linking of collagen and elastin and is essential for development of cardiovascular and respiratory systems, and development of skin and connective tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

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