Sigirr (NM_023059) Mouse Tagged ORF Clone
CAT#: MG206419
- TrueORF®
Sigirr (tGFP-tagged) - Mouse single immunoglobulin and toll-interleukin 1 receptor (TIR) domain (Sigirr)
ORF Plasmid: DDK
"NM_023059" in other vectors (4)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI256711; TIR8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206419 representing NM_023059
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGTGTCTGTGACATGGCCCCTAATTTCCTTTCCCCATCTGAAGACCAGGCCTTGGGTCTTGCCC TTGGCAGAGAAGTTGCTTTGAATTGCACAGCTTGGGTGTTCTCTAGGCCCCAGTGTCCCCAGCCATCAGT GCAGTGGCTGAAAGATGGTCTGGCATTGGGCAATGGAAGCCACTTCAGCCTCCATGAGGACTTCTGGGTC AGCGCCAACTTCTCAGAGATTGTGTCCAGTGTCCTGGTGCTCAACTTGACCAATGCAGAGGACTATGGAA CCTTCACCTGTTCTGTCTGGAATGTCAGCTCCCATTCCTTCACTCTTTGGCGAGCTGGCCCTGCTGGCCA TGTGGCTGCAGTACTGGCTTCCCTCCTGGTCCTGGTGGTTCTGCTGCTGGTGGCCCTGCTCTATGTTAAG TGTCGGCTGAACATGCTGCTTTGGTACCAAGACACTTACGGGGAGGTGGAGATGAACGATGGGAAGTTAT ACGATGCCTACGTGTCCTATAGCGACTGCCCAGAGGACCGCAAATTTGTAAATTTTATTCTGAAGCCTCA GTTGGAGCGGCGTCGGGGATACAAACTCTTCCTAGAGGACCGCGACCTCTTGCCTCGCGCGGAGCCCTCT GCCGACCTTTTGGTGAACCTGAGTCGCTGTCGGCGTCTCATCGTGGTTCTTTCAGATGCCTTCCTAAGCC GGCCCTGGTGTAGCCAGAGCTTCCGGGAGGGACTGTGCCGCCTACTGGAGCTCACCCGCAGACCTATCTT CATCACCTTTGAGGGCCAGAGGCGTGAGCCCATACACCCTGCTCTCCGGCTCCTGCGCCAGCACCGCCAC CTCGTGACCCTGGTGCTTTGGAAGCCTGGCTCCGTGACTCCTTCCTCTGATTTTTGGAAAGAGCTACAGC TAGCACTGCCACGGAAGGTGCAGTACAGGCCGGTGGAGGGAGACCCTCAAACCCGACTTCAGGATGACAA AGATCCCATGCTAATCGTGAGAGGACGTGCTGCCCAGGGCCGGGGCATGGAGTCAGAGCTGGATCCAGAC CCTGAGGGAGACCTGGGTGTCCGTGGACCTGTCTTTGGGGAGCCACCAACTCCACTGCAGGAAACCAGGA TCTGCATAGGAGAGAGCCACGGCAGTGAAATGGATGTCTCTGACCTCGGCTCTCGAAACTACAGTGCACG GACAGACTTCTACTGCCTCGTGTCTGAGGATGATGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206419 representing NM_023059
Red=Cloning site Green=Tags(s) MAGVCDMAPNFLSPSEDQALGLALGREVALNCTAWVFSRPQCPQPSVQWLKDGLALGNGSHFSLHEDFWV SANFSEIVSSVLVLNLTNAEDYGTFTCSVWNVSSHSFTLWRAGPAGHVAAVLASLLVLVVLLLVALLYVK CRLNMLLWYQDTYGEVEMNDGKLYDAYVSYSDCPEDRKFVNFILKPQLERRRGYKLFLEDRDLLPRAEPS ADLLVNLSRCRRLIVVLSDAFLSRPWCSQSFREGLCRLLELTRRPIFITFEGQRREPIHPALRLLRQHRH LVTLVLWKPGSVTPSSDFWKELQLALPRKVQYRPVEGDPQTRLQDDKDPMLIVRGRAAQGRGMESELDPD PEGDLGVRGPVFGEPPTPLQETRICIGESHGSEMDVSDLGSRNYSARTDFYCLVSEDDV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023059 |
ORF Size | 1227 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023059.3 |
RefSeq Size | 1993 bp |
RefSeq ORF | 1230 bp |
Locus ID | 24058 |
UniProt ID | Q9JLZ8 |
Gene Summary | Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205748 | Sigirr (untagged) - Mouse single immunoglobulin and toll-interleukin 1 receptor (TIR) domain (Sigirr), (10ug) |
CNY 3,656.00 |
|
MR206419 | Sigirr (Myc-DDK-tagged) - Mouse single immunoglobulin and toll-interleukin 1 receptor (TIR) domain (Sigirr) |
CNY 3,656.00 |
|
MR206419L3 | Lenti ORF clone of Sigirr (Myc-DDK-tagged) - Mouse single immunoglobulin and toll-interleukin 1 receptor (TIR) domain (Sigirr) |
CNY 4,750.00 |
|
MR206419L4 | Lenti ORF clone of Sigirr (mGFP-tagged) - Mouse single immunoglobulin and toll-interleukin 1 receptor (TIR) domain (Sigirr) |
CNY 4,750.00 |