Acy1 (NM_025371) Mouse Tagged ORF Clone
CAT#: MG206415
- TrueORF®
Acy1 (tGFP-tagged) - Mouse aminoacylase 1 (Acy1)
ORF Plasmid: DDK
"NM_025371" in other vectors (4)
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CNY 3,710.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110014J22Rik; Acy-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206415 representing NM_025371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCAAGGATCCCGAGTCTGAGCACCCCTCCGTGACGCTCTTCCGCCAGTACCTGCGCATCTGCA CTGTGCAACCCAATCCAGACTATGGAGGGGCCATCACCTTCCTGGAGGAGAGAGCCCGCCAGCTGGGTCT GAGCTGTCAGAAAATAGAGGTGGTGCCTGGCTTTGTGATCACTGTGCTGACCTGGCCAGGCACCAACCCG TCACTCCCCTCCATCTTGCTAAACTCCCACACAGATGTGGTACCTGTCTTCAAGGAACATTGGCATCACG ACCCCTTTGAGGCCTTCAAGGATTCCGAGGGCTACATCTATGCTAGGGGCTCCCAGGACATGAAGAGTGT CAGCATCCAGTACCTGGAAGCTGTGAGGAGGCTGAAGAGCGAGGGCCACCGATTCCCTAGGACCATCCAC ATGACCTTTGTGCCTGATGAGGAGGTCGGAGGTCACAAGGGGATGGAGTTGTTTGTGAAGAGGCCTGAGT TCCAGGCTCTGCGGGCAGGCTTCGCTCTGGACGAGGGCCTGGCCAACCCCACGGATGCCTTTACTGTCTT TTATAGTGAGCGGAGTCCTTGGTGGGTACGGGTTACCAGCACTGGGAAGCCAGGCCATGCCTCACGCTTC ATTGAGGATACAGCTGCAGAGAAGTTGCATAAGGTTATAAGCTCCATCTTGGCATTCCGGGAGAAGGAAA GGCAGAGGCTGCAGGCGAACCCTCACCTGAAGGAAGGCGCTGTGACTTCGGTGAATCTGACCAAGCTTGA AGGTGGCGTGGCCTATAATGTGGTACCTGCCACTATGAGTGCCAGCTTTGACTTCCGAGTGGCACCAGAT GTAGACATGAAGGCCTTCGAGAAGCAGCTGCAGAGATGGTGCCAGGAAGCGGGAGAGGGGGTCACCTTTG AGTTTGCTCAGAAGTTTACAGAGCCTCGAATGACACCCACGGATGATTCAGATCCCTGGTGGGCAGCTTT CAGCGGAGCCTGCAAGGCAATGAACCTCACTCTGGAGCCAGAGATCTTTCCTGCTGCCACTGACAGCCGC TATATCCGAGCGGTGGGGATCCCAGCTCTGGGCTTCTCGCCCATGAACCGCACACCTGTCCTGCTACATG ACCATAATGAACGGCTGCATGAGGATATATTTCTCCGTGGGGTTGACATATATACAGGCCTCCTCTCTGC ACTTGCTAGTGTGCCCACCCTGCCTGGTGAAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206415 representing NM_025371
Red=Cloning site Green=Tags(s) MTTKDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLTWPGTNP SLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEAVRRLKSEGHRFPRTIH MTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWVRVTSTGKPGHASRF IEDTAAEKLHKVISSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSASFDFRVAPD VDMKAFEKQLQRWCQEAGEGVTFEFAQKFTEPRMTPTDDSDPWWAAFSGACKAMNLTLEPEIFPAATDSR YIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALASVPTLPGES TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025371 |
ORF Size | 1224 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025371.1, NP_079647.1 |
RefSeq Size | 1432 bp |
RefSeq ORF | 1227 bp |
Locus ID | 109652 |
UniProt ID | Q99JW2 |
Gene Summary | Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Family-wide annotation of enzymatic pathways by parallel in vivo metabolomics
,null,
Cell chemical biology
,PubMed ID 31587987
[Acy1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200771 | Acy1 (untagged) - Mouse aminoacylase 1 (Acy1), (10ug) |
CNY 3,656.00 |
|
MR206415 | Acy1 (Myc-DDK-tagged) - Mouse aminoacylase 1 (Acy1) |
CNY 3,656.00 |
|
MR206415L3 | Lenti ORF clone of Acy1 (Myc-DDK-tagged) - Mouse aminoacylase 1 (Acy1) |
CNY 5,230.00 |
|
MR206415L4 | Lenti ORF clone of Acy1 (mGFP-tagged) - Mouse aminoacylase 1 (Acy1) |
CNY 5,230.00 |