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Bhmt (BC037004) Mouse Tagged ORF Clone
CAT＃: MG206399
- TrueORF®
Bhmt (tGFP-tagged) - Mouse betaine-homocysteine methyltransferase (cDNA clone MGC:46866 IMAGE:5100429)
ORF Plasmid: DDK
"BC037004" in other vectors (3)
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CNY 4,470.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC46866 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206399 representing BC037004
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCAGTTGCTGGCAAGAAGGCCAAGAAGGGGATCTTAGAACGCTTAAATGCCGGAGAAGTTGTGA TTGGAGATGGAGGATTTGTCTTTGCACTGGAAAAGAGGGGCTATGTAAAGGCTGGACCCTGGACCCCAGA AGCTGCCGTGGAGCATCCTGAGGCAGTTCGTCAGCTTCATCGGGAGTTCCTCAGAGCTGGATCGAACGTC ATGCAGACCTTCACTTTCTATGCAAGTGAAGACAAGCTGGAAAACAGAGGAAACTATGTGGCAGAGAAGA TTTCTGGGCAGAAAGTCAACGAAGCTGCTTGTGACATTGCACGGCAAGTGGCTGATGAAGGAGACGCTTT GGTTGCAGGAGGCGTGAGCCAGACGCCTTCATACCTTAGCTGCAAGAGTGAGGTAGAAGTGAAAAAGATA TTTCGCCAACAGCTAGAGGTGTTCATGAAGAAGAACGTGGACTTCCTCATTGCAGAGTATTTTGAACATG TTGAAGAAGCCGTGTGGGCAGTGGAAGCCTTAAAAGCATCTGGTAAGCCCGTAGCAGCTACCATGTGCAT TGGGCCCGAGGGAGATCTGCATGGCGTGCCCCCTGGAGAGTGTGCCGTGCGTCTGGTGAAAGCAGGCGCC TCCATTGTCGGCGTGAACTGCCACTTCGACCCCAGCGTCAGCTTACAGACTGTGAAGCTCATGAAGGAGG GTTTGGAGGCTGCGCGGTTGAAAGCTTACCTGATGAGCCAGCCCCTGGCCTACCATACCCCTGACTGTGG CAAACAGGGATTTATTGATCTCCCAGAATTCCCCTTTGGATTGGAACCCCGAGTTGCCACTAGATGGGAT ATTCAAAAATATGCCAGAGAGGCCTACAACCTGGGGGTTAGGTACATTGGCGGCTGCTGCGGATTTGAGC CCTACCACATCAGGGCGATTGCAGAGGAGTTGGCCCCAGAAAGGGGATTTTTGCCACCGGCTTCAGAAAA ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGATCAGAGCAAGGGCCAGAAAGGAA TACTGGCAGAATCTGCGAATAGCTTCCGGCAGGCCGTACAACCCTTCCATGTCCCGGCCAGATGCTTGGG GCGTGACTAAGGGAGCAGCCGAGCTGATGCAGCAGAAGGAGGCCACTACTGAGCAGCAGCTGAGAGAGCT CTTTGAAAAACAAAAATTCAAGTCTGCACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206399 representing BC037004
Red=Cloning site Green=Tags(s) MAPVAGKKAKKGILERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNV MQTFTFYASEDKLENRGNYVAEKISGQKVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSEVEVKKI FRQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGA SIVGVNCHFDPSVSLQTVKLMKEGLEAARLKAYLMSQPLAYHTPDCGKQGFIDLPEFPFGLEPRVATRWD IQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARKE YWQNLRIASGRPYNPSMSRPDAWGVTKGAAELMQQKEATTEQQLRELFEKQKFKSAQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC037004 |
| ORF Size | 1221 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC037004.1 |
| RefSeq Size | 1881 bp |
| RefSeq ORF | 1223 bp |
| Locus ID | 12116 |
| Gene Summary | Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206399 | Bhmt (Myc-DDK-tagged) - Mouse betaine-homocysteine methyltransferase (cDNA clone MGC:46866 IMAGE:5100429) |
CNY 3,656.00 |
|
| MR206399L3 | Lenti ORF clone of Bhmt (Myc-DDK-tagged) - Mouse betaine-homocysteine methyltransferase (cDNA clone MGC:46866 IMAGE:5100429) |
CNY 5,990.00 |
|
| MR206399L4 | Lenti ORF clone of Bhmt (mGFP-tagged) - Mouse betaine-homocysteine methyltransferase (cDNA clone MGC:46866 IMAGE:5100429) |
CNY 5,990.00 |
