Ptpn2 (BC008269) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI325124; Ptpt; TC-PTP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206379 representing BC008269 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCAACCATCGAGCGGGAGTTCGAGGAACTGGATGCTCAGTGTCGCTGGCAGCCGTTATACTTGG AAATTCGAAATGAATCCCATGACTATCCTCATAGAGTGGCCAAGTTTCCAGAAAACAGAAACCGAAACAG ATACAGAGATGTAAGCCCATATGATCACAGTCGTGTTAAACTGCAAAGTACTGAAAATGATTATATTAAT GCCAGCTTAGTTGACATAGAAGAGGCACAAAGAAGTTACATCTTAACACAGGGCCCACTTCCGAACACAT GCTGCCATTTCTGGCTCATGGTGTGGCAGCAAAAGACCAAAGCAGTTGTCATGCTAAACCGAACTGTAGA AAAAGAATCGGTTAAATGTGCACAGTACTGGCCAACGGATGACAGAGAAATGGTGTTTAAGGAAACGGGA TTCAGTGTGAAGCTCTTATCTGAAGATGTAAAATCATATTATACAGTACATCTACTACAGTTAGAAAATA TCAATACTGGTGAAACCAGAACCATATCTCACTTCCATTATACCACCTGGCCAGATTTTGGGGTTCCAGA GTCACCAGCTTCATTTCTAAACTTCTTGTTTAAAGTTAGAGAATCTGGTTGTTTGACCCCTGACCATGGA CCTGCAGTGATCCATTGCAGTGCGGGCATCGGGCGCTCTGGCACCTTCTCTCTTGTAGATACCTGTCTTG TTCTGATGGAAAAAGGAGAGGATGTTAATGTGAAACAATTATTACTGAATATGAGAAAGTATCGAATGGG ACTTATTCAGACACCGGACCAACTCAGATTCTCCTACATGGCCATAATAGAAGGAGCAAAGTACACAAAA GGAGATTCAAATATACAGAAACGGTGGAAAGAACTTTCTAAAGAAGATTTATCTCCTATTTGTGATCATT CACAGAACAGAGTGATGGTTGAGAAGTACAATGGGAAGAGAATAGGTTCAGAAGATGAAAAGTTAACAGG GCTTCCTTCTAAGGTGCAGGATACTGTGGAGGAGAGCAGTGAGAGCATTCTACGGAAACGTATTCGAGAG GATAGAAAGGCTACGACGGCTCAGAAGGTGCAGCAGATGAAACAGAGGCTAAATGAAACTGAACGAAAAA GAAAAAGGTGGTTATATTGGCAACCTATTCTCACTAAGATGGGGTTTGTGTCAGTCATTTTGGTTGGCGC TTTGGTTGGCTGGACACTGCTTTTTCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206379 representing BC008269 Red=Cloning site Green=Tags(s) MSATIEREFEELDAQCRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQSTENDYIN ASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRTVEKESVKCAQYWPTDDREMVFKETG FSVKLLSEDVKSYYTVHLLQLENINTGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGCLTPDHG PAVIHCSAGIGRSGTFSLVDTCLVLMEKGEDVNVKQLLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKYTK GDSNIQKRWKELSKEDLSPICDHSQNRVMVEKYNGKRIGSEDEKLTGLPSKVQDTVEESSESILRKRIRE DRKATTAQKVQQMKQRLNETERKRKRWLYWQPILTKMGFVSVILVGALVGWTLLFH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC008269
ORF Size	1218 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008269, AAH08269
RefSeq Size	1906 bp
RefSeq ORF	1220 bp
Locus ID	19255
Gene Summary	Non-receptor type tyrosine-specific phosphatase that dephosphorylates receptor protein tyrosine kinases including INSR, EGFR, CSF1R, PDGFR. Also dephosphorylates non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3 and STAT6 either in the nucleus or the cytoplasm. Negatively regulates numerous signaling pathways and biological processes like hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. Plays a multifaceted and important role in the development of the immune system. Functions in T-cell receptor signaling through dephosphorylation of FYN and LCK to control T-cells differentiation and activation. Dephosphorylates CSF1R, negatively regulating its downstream signaling and macrophage differentiation. Negatively regulates cytokine (IL2/interleukin-2 and interferon)-mediated signaling through dephosphorylation of the cytoplasmic kinases JAK1, JAK3 and their substrate STAT1, that propagate signaling downstream of the cytokine receptors. Also regulates the IL6/interleukin-6 and IL4/interleukin-4 cytokine signaling through dephosphorylation of STAT3 and STAT6 respectively. In addition to the immune system, it is involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth. Activated by the integrin ITGA1/ITGB1, it dephosphorylates EGFR and negatively regulates EGF signaling. Dephosphorylates PDGFRB and negatively regulates platelet-derived growth factor receptor-beta signaling pathway and therefore cell proliferation. Negatively regulates tumor necrosis factor-mediated signaling downstream via MAPK through SRC dephosphorylation. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of the hepatocyte growth factor receptor MET. Plays also an important role in glucose homeostasis. For instance, negatively regulates the insulin receptor signaling pathway through the dephosphorylation of INSR and control gluconeogenesis and liver glucose production through negative regulation of the IL6 signaling pathways. May also bind DNA.[UniProtKB/Swiss-Prot Function]

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