Gcnt3 (BC018297) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2010013H22Rik; 2210021I22Rik; 2210401J11Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206243 representing BC018297 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGGACTCTGAGGAATTTCAAAGCCAGTACTGCAGGGATCTCCTGTACAAGACCCTGAAGCTGC CAGCCAAGAGTTCCATCAACTGCTCAGGGGTCATTCGAGGGGAGCAGAAAGCGGTGACCCAGGCTCTGCT GAATAACCTGGAAATTAAGAAGAAGCAGCAGCTCTTCACAGAGGCCGACTACCTTAGGATGACAGCAGAC TGTGAGCACTTCAAGACCAAGAGGAAGTTTATACAGGTCCCACTGAGCAAGGAAGAGGCCAGCTTCCCCA TTGCGTACTCCATGGTGGTGCATGAGAAGATTGAGAACTTCGAAAGGTTGCTGCGAGCTGTGTACACCCC TCAGAATGTATACTGTGTCCACATGGATCAGAAGTCTTCAGAACCCTTTAAGCAGGCAGTCAGGGCCATC GTGTCATGCTTCCCCAATGTCTTCATAGCTAGTAAGTTGGTGTCAGTGGTCTATGCTTCCTGGTCCAGGG TGCAGGCTGACCTAAACTGCATGGAAGACTTGCTTCAGAGCCCCGTGCCATGGAAATACCTCCTGAACAC CTGTGGGACAGACTTTCCCATCAAAACCAATGCTGAGATGGTCAAGGCCCTCAAGCTATTGAAAGGGCAG AACAGTATGGAGTCAGAGGTACCACCTCCACATAAAAAATCCCGCTGGAAATATCACTATGAGGTGACAG ACACATTGCACATGACCAGCAAGAGGAAGACGCCGCCACCTAATAACCTAACCATGTTCACTGGGAATGC CTACATGGTGGCTTCTCGAGACTTCATTGAACACGTGTTCAGTAACTCAAAAGCCCGGCAACTGATCGAG TGGGTAAAAGACACCTATAGTCCCGATGAGCACCTTTGGGCCACCCTCCAGCGTGCCTCGTGGATGCCTG GATCAGATCCCTTGCATCGAAAATTTGACCTGTCAGACATGAGAGCCATTGCGAGACTAACCAAGTGGTA CGACCATGAGGGAGACATTGAGAACGGGGCACCTTACACGTCTTGCTCAGGAATCCACCAGCGGGCTGTC TGTGTTTATGGGTCAGGGGACCTGCACTGGATACTTCAGAACCATCACCTCTTGGCCAACAAGTTTGACC CAAAGGTGGATGATAATGTTCTTCAGTGTTTAGAAGAATATTTACGTCACAAAGCCATCTATGGGACTGA ACTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206243 representing BC018297 Red=Cloning site Green=Tags(s) MDLDSEEFQSQYCRDLLYKTLKLPAKSSINCSGVIRGEQKAVTQALLNNLEIKKKQQLFTEADYLRMTAD CEHFKTKRKFIQVPLSKEEASFPIAYSMVVHEKIENFERLLRAVYTPQNVYCVHMDQKSSEPFKQAVRAI VSCFPNVFIASKLVSVVYASWSRVQADLNCMEDLLQSPVPWKYLLNTCGTDFPIKTNAEMVKALKLLKGQ NSMESEVPPPHKKSRWKYHYEVTDTLHMTSKRKTPPPNNLTMFTGNAYMVASRDFIEHVFSNSKARQLIE WVKDTYSPDEHLWATLQRASWMPGSDPLHRKFDLSDMRAIARLTKWYDHEGDIENGAPYTSCSGIHQRAV CVYGSGDLHWILQNHHLLANKFDPKVDDNVLQCLEEYLRHKAIYGTEL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC018297
ORF Size	1196 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018297, AAH18297
RefSeq Size	1841 bp
RefSeq ORF	1196 bp
Locus ID	72077
Gene Summary	Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.[UniProtKB/Swiss-Prot Function]

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