Gcnt3 (BC018297) Mouse Tagged ORF Clone
CAT#: MG206243
- TrueORF®
Gcnt3 (tGFP-tagged) - Mouse glucosaminyl (N-acetyl) transferase 3, mucin type (cDNA clone MGC:18826 IMAGE:4208500)
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CNY 3,710.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2010013H22Rik; 2210021I22Rik; 2210401J11Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206243 representing BC018297
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGGACTCTGAGGAATTTCAAAGCCAGTACTGCAGGGATCTCCTGTACAAGACCCTGAAGCTGC CAGCCAAGAGTTCCATCAACTGCTCAGGGGTCATTCGAGGGGAGCAGAAAGCGGTGACCCAGGCTCTGCT GAATAACCTGGAAATTAAGAAGAAGCAGCAGCTCTTCACAGAGGCCGACTACCTTAGGATGACAGCAGAC TGTGAGCACTTCAAGACCAAGAGGAAGTTTATACAGGTCCCACTGAGCAAGGAAGAGGCCAGCTTCCCCA TTGCGTACTCCATGGTGGTGCATGAGAAGATTGAGAACTTCGAAAGGTTGCTGCGAGCTGTGTACACCCC TCAGAATGTATACTGTGTCCACATGGATCAGAAGTCTTCAGAACCCTTTAAGCAGGCAGTCAGGGCCATC GTGTCATGCTTCCCCAATGTCTTCATAGCTAGTAAGTTGGTGTCAGTGGTCTATGCTTCCTGGTCCAGGG TGCAGGCTGACCTAAACTGCATGGAAGACTTGCTTCAGAGCCCCGTGCCATGGAAATACCTCCTGAACAC CTGTGGGACAGACTTTCCCATCAAAACCAATGCTGAGATGGTCAAGGCCCTCAAGCTATTGAAAGGGCAG AACAGTATGGAGTCAGAGGTACCACCTCCACATAAAAAATCCCGCTGGAAATATCACTATGAGGTGACAG ACACATTGCACATGACCAGCAAGAGGAAGACGCCGCCACCTAATAACCTAACCATGTTCACTGGGAATGC CTACATGGTGGCTTCTCGAGACTTCATTGAACACGTGTTCAGTAACTCAAAAGCCCGGCAACTGATCGAG TGGGTAAAAGACACCTATAGTCCCGATGAGCACCTTTGGGCCACCCTCCAGCGTGCCTCGTGGATGCCTG GATCAGATCCCTTGCATCGAAAATTTGACCTGTCAGACATGAGAGCCATTGCGAGACTAACCAAGTGGTA CGACCATGAGGGAGACATTGAGAACGGGGCACCTTACACGTCTTGCTCAGGAATCCACCAGCGGGCTGTC TGTGTTTATGGGTCAGGGGACCTGCACTGGATACTTCAGAACCATCACCTCTTGGCCAACAAGTTTGACC CAAAGGTGGATGATAATGTTCTTCAGTGTTTAGAAGAATATTTACGTCACAAAGCCATCTATGGGACTGA ACTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206243 representing BC018297
Red=Cloning site Green=Tags(s) MDLDSEEFQSQYCRDLLYKTLKLPAKSSINCSGVIRGEQKAVTQALLNNLEIKKKQQLFTEADYLRMTAD CEHFKTKRKFIQVPLSKEEASFPIAYSMVVHEKIENFERLLRAVYTPQNVYCVHMDQKSSEPFKQAVRAI VSCFPNVFIASKLVSVVYASWSRVQADLNCMEDLLQSPVPWKYLLNTCGTDFPIKTNAEMVKALKLLKGQ NSMESEVPPPHKKSRWKYHYEVTDTLHMTSKRKTPPPNNLTMFTGNAYMVASRDFIEHVFSNSKARQLIE WVKDTYSPDEHLWATLQRASWMPGSDPLHRKFDLSDMRAIARLTKWYDHEGDIENGAPYTSCSGIHQRAV CVYGSGDLHWILQNHHLLANKFDPKVDDNVLQCLEEYLRHKAIYGTEL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC018297 |
ORF Size | 1196 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC018297, AAH18297 |
RefSeq Size | 1841 bp |
RefSeq ORF | 1196 bp |
Locus ID | 72077 |
Gene Summary | Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.[UniProtKB/Swiss-Prot Function] |
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