Nr2f6 (BC008138) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	EAR2, COUP-TF3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206083 representing BC008138 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGTGACCGGTGGCTGGGGCGACCCCGGAGGCGACACGAACGGCGTGGACAAGGCTGGTGGGA GCTACCCACGCGCGACCGAGGACGATTCGGCGTCACCTCCCGGGGCGACCAGCGACGCGGAGCCGGGCGA CGAGGAGCGTCCGGGGTTGCAGGTGGACTGCGTGGTGTGCGGGGACAAGTCCAGTGGAAAGCATTACGGC GTGTTCACCTGCGAGGGCTGCAAGAGTTTCTTCAAGCGCAGCATCCGCCGCAATCTCAGCTACACCTGCC GGTCCAACCGTGACTGTCAGATTGATCAGCACCACCGGAACCAGTGTCAGTACTGTCGGCTCAAGAAGTG CTTCCGGGTGGGCATGCGCAAGGAGGCCGTGCAGCGAGGCCGCATCCCGCATGCGCTCCCCGGTCCAGCG GCCTGCAGTCCCCCGGGCGCGACGGGCGTCGAACCTTTCACGGGGCCGCCAGTGTCCGAGCTGATTGCGC AGCTGCTGCGTGCTGAGCCCTACCCCGCGGCCGGACGCTTTGGTGGCGGCGGCGCTGTACTGGGCATCGA CAACGTGTGCGAGTTGGCGGCACGCCTGCTGTTCAGCACGGTCGAGTGGGCCCGCCACGCGCCCTTCTTC CCCGAGCTGCCGGCCGCCGACCAGGTGGCGCTGCTGCGGCTCAGCTGGAGTGAGCTCTTCGTGCTGAACG CGGCGCAGGCGGCGCTGCCGCTGCATACGGCACCGCTGCTGGCCGCCGCGGGGTTGCATGCCGCGCCCAT GGCAGCCGAGCGGGCCGTGGCCTTCATGGACCAGGTGCGTGCCTTCCAGGAGCAGGTGGACAAGCTGGGC CGCCTGCAGGTGGATGCTGCGGAGTACGGCTGCCTCAAGGCCATCGCGCTCTTCACGCCTGATGCCTGTG GCCTTTCTGACCCAGCCCATGTGGAGAGCCTGCAGGAGAAGGCACAGGTGGCCCTCACCGAGTATGTGCG TGCCCAGTACCCATCGCAGCCCCAGCGCTTTGGGCGTCTGCTGCTGCGGCTGCCAGCCCTGCGTGCTGTG CCCGCATCCCTCATCTCCCAGCTCTTCTTCATGCGCCTGGTGGGCAAGACACCCATCGAGACCCTCATCC GGGACATGCTTCTGTCAGGGAGCACCTTTAACTGGCCCTATGGCTCGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206083 representing BC008138 Red=Cloning site Green=Tags(s) MAMVTGGWGDPGGDTNGVDKAGGSYPRATEDDSASPPGATSDAEPGDEERPGLQVDCVVCGDKSSGKHYG VFTCEGCKSFFKRSIRRNLSYTCRSNRDCQIDQHHRNQCQYCRLKKCFRVGMRKEAVQRGRIPHALPGPA ACSPPGATGVEPFTGPPVSELIAQLLRAEPYPAAGRFGGGGAVLGIDNVCELAARLLFSTVEWARHAPFF PELPAADQVALLRLSWSELFVLNAAQAALPLHTAPLLAAAGLHAAPMAAERAVAFMDQVRAFQEQVDKLG RLQVDAAEYGCLKAIALFTPDACGLSDPAHVESLQEKAQVALTEYVRAQYPSQPQRFGRLLLRLPALRAV PASLISQLFFMRLVGKTPIETLIRDMLLSGSTFNWPYGSG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC008138
ORF Size	1170 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008138, AAH08138
RefSeq Size	1959 bp
RefSeq ORF	1172 bp
Locus ID	13864
Gene Summary	Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).[UniProtKB/Swiss-Prot Function]

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