Nr2f6 (BC008138) Mouse Tagged ORF Clone

CAT#: MG206083

  • TrueORF®

Nr2f6 (tGFP-tagged) - Mouse nuclear receptor subfamily 2, group F, member 6 (cDNA clone MGC:6088 IMAGE:3582557)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms EAR2, COUP-TF3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206083 representing BC008138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATGGTGACCGGTGGCTGGGGCGACCCCGGAGGCGACACGAACGGCGTGGACAAGGCTGGTGGGA
GCTACCCACGCGCGACCGAGGACGATTCGGCGTCACCTCCCGGGGCGACCAGCGACGCGGAGCCGGGCGA
CGAGGAGCGTCCGGGGTTGCAGGTGGACTGCGTGGTGTGCGGGGACAAGTCCAGTGGAAAGCATTACGGC
GTGTTCACCTGCGAGGGCTGCAAGAGTTTCTTCAAGCGCAGCATCCGCCGCAATCTCAGCTACACCTGCC
GGTCCAACCGTGACTGTCAGATTGATCAGCACCACCGGAACCAGTGTCAGTACTGTCGGCTCAAGAAGTG
CTTCCGGGTGGGCATGCGCAAGGAGGCCGTGCAGCGAGGCCGCATCCCGCATGCGCTCCCCGGTCCAGCG
GCCTGCAGTCCCCCGGGCGCGACGGGCGTCGAACCTTTCACGGGGCCGCCAGTGTCCGAGCTGATTGCGC
AGCTGCTGCGTGCTGAGCCCTACCCCGCGGCCGGACGCTTTGGTGGCGGCGGCGCTGTACTGGGCATCGA
CAACGTGTGCGAGTTGGCGGCACGCCTGCTGTTCAGCACGGTCGAGTGGGCCCGCCACGCGCCCTTCTTC
CCCGAGCTGCCGGCCGCCGACCAGGTGGCGCTGCTGCGGCTCAGCTGGAGTGAGCTCTTCGTGCTGAACG
CGGCGCAGGCGGCGCTGCCGCTGCATACGGCACCGCTGCTGGCCGCCGCGGGGTTGCATGCCGCGCCCAT
GGCAGCCGAGCGGGCCGTGGCCTTCATGGACCAGGTGCGTGCCTTCCAGGAGCAGGTGGACAAGCTGGGC
CGCCTGCAGGTGGATGCTGCGGAGTACGGCTGCCTCAAGGCCATCGCGCTCTTCACGCCTGATGCCTGTG
GCCTTTCTGACCCAGCCCATGTGGAGAGCCTGCAGGAGAAGGCACAGGTGGCCCTCACCGAGTATGTGCG
TGCCCAGTACCCATCGCAGCCCCAGCGCTTTGGGCGTCTGCTGCTGCGGCTGCCAGCCCTGCGTGCTGTG
CCCGCATCCCTCATCTCCCAGCTCTTCTTCATGCGCCTGGTGGGCAAGACACCCATCGAGACCCTCATCC
GGGACATGCTTCTGTCAGGGAGCACCTTTAACTGGCCCTATGGCTCGGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206083 representing BC008138
Red=Cloning site Green=Tags(s)

MAMVTGGWGDPGGDTNGVDKAGGSYPRATEDDSASPPGATSDAEPGDEERPGLQVDCVVCGDKSSGKHYG
VFTCEGCKSFFKRSIRRNLSYTCRSNRDCQIDQHHRNQCQYCRLKKCFRVGMRKEAVQRGRIPHALPGPA
ACSPPGATGVEPFTGPPVSELIAQLLRAEPYPAAGRFGGGGAVLGIDNVCELAARLLFSTVEWARHAPFF
PELPAADQVALLRLSWSELFVLNAAQAALPLHTAPLLAAAGLHAAPMAAERAVAFMDQVRAFQEQVDKLG
RLQVDAAEYGCLKAIALFTPDACGLSDPAHVESLQEKAQVALTEYVRAQYPSQPQRFGRLLLRLPALRAV
PASLISQLFFMRLVGKTPIETLIRDMLLSGSTFNWPYGSG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC008138
ORF Size 1170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC008138, AAH08138
RefSeq Size 1959 bp
RefSeq ORF 1172 bp
Locus ID 13864
Gene Summary Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...