Sav1 (BC019377) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Wwp3, WW45, Wwp4, Sav
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206033 representing BC019377 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCCGCAAGAAAACCAAAAACGAGGTGTCTAAGCCGGCCGAGGTGCAGGGCAAGTACGTGAAGA AGGAGACGTCGCCCCTGCTGCGGAATCTCATGCCTTCATTCATTCGGCACGGTCCAACAATTCCCAGACG GACTGACCTCTGTCTTCCAGATTCAAGTGCTACTGCTTTCTCAGCTTCTGGAGACGGTATAGTTTCAAGA AACCAGAGTTTCCTGAGAACTGCAATTCAAAGGACACCTCATGAAGTAATGAGAAGAGAAAGCCACAGAC TGTCTGCCCCTTCTTACCTTGTCAGGAGCCTAGCAGATGTCCCTCGAGAGTGTGGCTCATCACAGTCATT TTTGACAGAAGTTAACTTTGCTGTTGAGAATGGAGACTCTGGCTCCCGATACTTCTTCTCAGATAACTTT TTTGATGGACAGAGAAGGCGGCCACTTGGAGATCGTGCACAAGAAGATTACAGATATTATGAATACAACC ATGATCTCTTCCAGAGGATGCCACAGAGTCAGGGGAGGCACACTTCAGGTATTGGGAGAGTCACGGCTAC ATCTCTAGGGAATTTAACTAACCATGGATCTGAAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGG ACAATGAGAGGGAGAAAATACTACATAGATCATAACACAAATACCACTCACTGGAGTCATCCCCTTGAAC GAGAAGGACTTCCTCCTGGCTGGGAACGAGTAGAGTCATCAGAATTTGGAACCTATTACGTGGATCACAC CAATAAAAGGGCTCAGTACAGGCACCCCTGTGCTCCGAGTGTACCTCGGTATGATCAGCCTCCACCCATC ACGTATCAGCCACAACAAACTGAAAGAAATCAGTCTCTCCTGGTCCCTGCAAATCCCTACCATACTGCAG AAATTCCTGACTGGCTTCAGGTTTATGCCCGAGCCCCTGTGAAATATGACCACATTCTGAAGTGGGAGCT CTTCCAGCTGGCTGACCTGGACACGTACCAGGGAATGCTGAAGTTGCTCTTCATGAAGGAACTGGAGCAG ATTGTGAAGTTGTACGAGGCCTACAGACAGGCTCTTCTCACTGAGTTGGAAAACCGCAAGCAGAGGCAGC AGTGGTATGCCCAGCAGCATGGCAAGACGTTCTTAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206033 representing BC019377 Red=Cloning site Green=Tags(s) MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDLCLPDSSATAFSASGDGIVSR NQSFLRTAIQRTPHEVMRRESHRLSAPSYLVRSLADVPRECGSSQSFLTEVNFAVENGDSGSRYFFSDNF FDGQRRRPLGDRAQEDYRYYEYNHDLFQRMPQSQGRHTSGIGRVTATSLGNLTNHGSEDLPLPPGWSVDW TMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKRAQYRHPCAPSVPRYDQPPPI TYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQ IVKLYEAYRQALLTELENRKQRQQWYAQQHGKTFLS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC019377
ORF Size	1160 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019377, AAH19377
RefSeq Size	1452 bp
RefSeq ORF	1160 bp
Locus ID	64010
Gene Summary	Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]

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