Sav1 (BC019377) Mouse Tagged ORF Clone

CAT#: MG206033

  • TrueORF®

Sav1 (tGFP-tagged) - Mouse salvador homolog 1 (Drosophila) (cDNA clone MGC:30471 IMAGE:4021056)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Wwp3, WW45, Wwp4, Sav
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206033 representing BC019377
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTCCCGCAAGAAAACCAAAAACGAGGTGTCTAAGCCGGCCGAGGTGCAGGGCAAGTACGTGAAGA
AGGAGACGTCGCCCCTGCTGCGGAATCTCATGCCTTCATTCATTCGGCACGGTCCAACAATTCCCAGACG
GACTGACCTCTGTCTTCCAGATTCAAGTGCTACTGCTTTCTCAGCTTCTGGAGACGGTATAGTTTCAAGA
AACCAGAGTTTCCTGAGAACTGCAATTCAAAGGACACCTCATGAAGTAATGAGAAGAGAAAGCCACAGAC
TGTCTGCCCCTTCTTACCTTGTCAGGAGCCTAGCAGATGTCCCTCGAGAGTGTGGCTCATCACAGTCATT
TTTGACAGAAGTTAACTTTGCTGTTGAGAATGGAGACTCTGGCTCCCGATACTTCTTCTCAGATAACTTT
TTTGATGGACAGAGAAGGCGGCCACTTGGAGATCGTGCACAAGAAGATTACAGATATTATGAATACAACC
ATGATCTCTTCCAGAGGATGCCACAGAGTCAGGGGAGGCACACTTCAGGTATTGGGAGAGTCACGGCTAC
ATCTCTAGGGAATTTAACTAACCATGGATCTGAAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGG
ACAATGAGAGGGAGAAAATACTACATAGATCATAACACAAATACCACTCACTGGAGTCATCCCCTTGAAC
GAGAAGGACTTCCTCCTGGCTGGGAACGAGTAGAGTCATCAGAATTTGGAACCTATTACGTGGATCACAC
CAATAAAAGGGCTCAGTACAGGCACCCCTGTGCTCCGAGTGTACCTCGGTATGATCAGCCTCCACCCATC
ACGTATCAGCCACAACAAACTGAAAGAAATCAGTCTCTCCTGGTCCCTGCAAATCCCTACCATACTGCAG
AAATTCCTGACTGGCTTCAGGTTTATGCCCGAGCCCCTGTGAAATATGACCACATTCTGAAGTGGGAGCT
CTTCCAGCTGGCTGACCTGGACACGTACCAGGGAATGCTGAAGTTGCTCTTCATGAAGGAACTGGAGCAG
ATTGTGAAGTTGTACGAGGCCTACAGACAGGCTCTTCTCACTGAGTTGGAAAACCGCAAGCAGAGGCAGC
AGTGGTATGCCCAGCAGCATGGCAAGACGTTCTTAAGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206033 representing BC019377
Red=Cloning site Green=Tags(s)

MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDLCLPDSSATAFSASGDGIVSR
NQSFLRTAIQRTPHEVMRRESHRLSAPSYLVRSLADVPRECGSSQSFLTEVNFAVENGDSGSRYFFSDNF
FDGQRRRPLGDRAQEDYRYYEYNHDLFQRMPQSQGRHTSGIGRVTATSLGNLTNHGSEDLPLPPGWSVDW
TMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKRAQYRHPCAPSVPRYDQPPPI
TYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQ
IVKLYEAYRQALLTELENRKQRQQWYAQQHGKTFLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC019377
ORF Size 1160 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC019377, AAH19377
RefSeq Size 1452 bp
RefSeq ORF 1160 bp
Locus ID 64010
Gene Summary Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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