B4galt6 (BC011149) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA536803; AU022389
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205973 representing BC011149 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGCTCAAGCGGATGATGCGGGTCTCCAATCGCTCTCTGATTGCCTTCATCTTCTTCTTCTCCC TCTCCACGTCCTGTCTCTACTTCATCTATGTGGCTCCAGGCATCGCCAACACGTACCTCTTTATGGTACA AGCTAGAGGTATCATGCTGAGAGAGAATGTGAAAACAATTGGACACATGATCAGGCTGTATACCAATAAA AACACTACGCTCAACGGAACAGATTATCCTGAAGGCAATAATACAAGTGACTATCTTGTTCAAACAACAA CGTATCTTCCGCAAAACTTCACGTATTTGCCACACCTCCCCTGTCCAGAGAAGCTGCCTTACATGCGGGG ATTCCTCAGTGTCAATGTGAGTGAGATCAGTTTTGATGAAGTCCATCAGCTCTTCTCCAAGGACTCAGAG ATTGGGCCAGGAGGCCACTGGAGGCCCAAAGACTGCAAGCCCAGATGGAAGGTGGCAGTTCTCATCCCCT TCCGTAACCGCCATGAACATCTTCCGATTTTTTTCCTGCACCTGATTCCGATGCTCCAGAAACAGCGGCT GGAATTTGCCTTTTATGTCATCGAACAGACCGGCACACAACCATTCAATCGTGCGATGCTCTTTAATGTG GGTTTCAAAGAGGCCATGAAAGACAGAGCCTGGGACTGTGTGATCTTCCATGATGTGGATCATCTGCCTG AAAATGATCGGAACTACTACGGATGTGGAGAGATGCCGCGGCACTTTGCAGCGAAGCTGGACAAGTACAT GTACATTCTTCCCTATAAGGAGTTTTTTGGTGGGGTGAGTGGCCTGACGGTGGAGCAATTCAGGAAGATC AACGGCTTTCCCAATGCCTTCTGGGGATGGGGAGGCGAGGACGATGACCTTTGGAACAGAGTTCACTACG CCGGCTACAATGTAACCCGGCCAGAAGGGGACTTGGGAAAATACATATCTATTCCTCATCACCATCGAGG GGAGGTACAGTTCCTAGGACGGTATAAATTACTTCGGTATTCCAAGGAGCGGCAGTACATCGATGGACTG AACAATTTATTATACACACCCAAAATACTGGTTGATAGGCTGTATACAAATATATCTGTAAACCTCATGC CAGAATTAGCTCCAATTGAAGACTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205973 representing BC011149 Red=Cloning site Green=Tags(s) MSALKRMMRVSNRSLIAFIFFFSLSTSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNK NTTLNGTDYPEGNNTSDYLVQTTTYLPQNFTYLPHLPCPEKLPYMRGFLSVNVSEISFDEVHQLFSKDSE IGPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNV GFKEAMKDRAWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKI NGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYISIPHHHRGEVQFLGRYKLLRYSKERQYIDGL NNLLYTPKILVDRLYTNISVNLMPELAPIEDY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC011149
ORF Size	1148 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011149, AAH11149
RefSeq Size	1733 bp
RefSeq ORF	1148 bp
Locus ID	56386
Gene Summary	Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:23882130, PubMed:30114188). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (PubMed:30114188).[UniProtKB/Swiss-Prot Function]

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