Tia1 (BC046812) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2310050N03Rik; AI256674; mTIA-1; TIA-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205879 representing BC046812 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGAGATGCCCAAGACTCTATACGTCGGTAACCTTTCTCGAGATGTGACAGAAGCTCTCATCC TCCAGCTCTTTAGCCAGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATGTAAGGACAGCAGGAAA TGACCCATACTGTTTTGTGGAGTTCCATGAGCACCGTCATGCAGCTGCAGCCCTGGCTGCCATGAATGGG CGGAAGATAATGGGTAAGGAAGTGAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAGGATACGA GCAATCATTTCCATGTGTTTGTTGGTGACCTCAGTCCAGAAATCACAACCGAAGACATCAAAGCAGCGTT TGCACCATTTGGAAGAATTTCAGATGCCCGTGTGGTAAAAGACATGGCTACCGGGAAGTCTAAGGGATAT GGCTTTGTCTCCTTTTTCAACAAATGGGATGCAGAAAATGCCATTCAGCAGATGGGTGGCCAGTGGCTTG GTGGAAGACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCAGCTCCAAAGAGTACATATGAGTCGAA CACCAAACAGCTGTCATATGATGAGGTTGTGAGTCAGTCTAGTCCCAACAACTGCACTGTGTACTGTGGA GGGGTGACGTCAGGACTGACAGAACAACTAATGCGTCAGACTTTTTCTCCATTTGGACAAATAATGGAAA TTCGAGTCTTCCCAGATAAAGGATACTCATTTGTTCGGTTCAGTTCCCATGAAAGTGCAGCACATGCGAT TGTTTCTGTTAATGGTACTACCATTGAAGGGCATGTGGTGAAATGTTATTGGGGCAAAGAGACTCTTGAT ATGATAAACCCTGTGCAACAGCAAAATCAAATTGGATATCCACCAACATATGGCCAGTGGGGCCAGTGGT ATGGAAATGCACAACAGATTGGCCAGTATGTGCCTAACGGTTGGCAAGTACCTGCCTATGGAGTGTATGG CCAGCCATGGAGCCAGCAGGGGTTCAATCAGACCCAGTCTTCTGCACCGTGGATGGGACCCAATTACAGT GTGCCACCACCTCAAGGGCAGAATGGCAGCATGTTGCCTAGTCAGCCTGCTGGGTATCGAGTGGCCGGGT ATGAAACCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205879 representing BC046812 Red=Cloning site Green=Tags(s) MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDVRTAGNDPYCFVEFHEHRHAAAALAAMNG RKIMGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGY GFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSSPNNCTVYCG GVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFSSHESAAHAIVSVNGTTIEGHVVKCYWGKETLD MINPVQQQNQIGYPPTYGQWGQWYGNAQQIGQYVPNGWQVPAYGVYGQPWSQQGFNQTQSSAPWMGPNYS VPPPQGQNGSMLPSQPAGYRVAGYETQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC046812
ORF Size	1133 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046812, AAH46812
RefSeq Size	3125 bp
RefSeq ORF	1133 bp
Locus ID	21841
Gene Summary	Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]

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