Nck1 (NM_010878) Mouse Tagged ORF Clone
CAT#: MG205875
- TrueORF®
Nck1 (tGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1)
ORF Plasmid: DDK
"NM_010878" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6330586M15Rik; D230010O13Rik; Nck; Nck-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205875 representing NM_010878
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGAAGTGGTGGTGGTGGCCAAATTTGATTATGTGGCACAGCAGGAACAAGAGCTGGATATCA AGAAGAATGAGCGATTATGGCTCCTGGATGACTCTAAATCCTGGTGGCGAGTTCGAAATTCCATGAATAA AACAGGTTTTGTCCCTTCTAACTATGTGGAAAGAAAAAACAGTGCTCGGAAAGCATCTATTGTTAAAAAC CTGAAGGACACCTTAGGTATTGGAAAAGTGAAAAGAAAACCCAGTGTTCCAGATACTGCATCTCCTGCTG ATGATAGCTTTGTTGATCCAGGAGAACGTCTCTATGACCTTAACATGCCTGCTTTTGTGAAATTTAACTA CATGGCTGAGAGAGAGGATGAGTTGTCATTGATAAAAGGGACCAAGGTGATCGTCATGGAGAAATGCAGT GATGGATGGTGGCGTGGCAGCTACAACGGACAAATTGGATGGTTTCCTTCAAACTATGTAACTGAAGAAG GTGACAGTCCTTTGGGTGATCATGTAGGTTCTCTGTCAGAGAAATTAGCAGCAGTTGTCAATAACCTAAA TACGGGTCAAGTATTGCATGTTGTACAGGCTCTTTACCCGTTTAGCTCATCCAATGATGAAGAACTCAAT TTTGAGAAAGGCGATGTAATGGATGTTATTGAAAAGCCGGAAAATGACCCAGAGTGGTGGAAATGCAGGA AAATCAATGGCATGGTTGGCCTGGTGCCAAAAAACTACGTTACCATTATGCAAAACAATCCATTAACCTC AGGTTTGGAACCATCTCCTCCACAATGTGATTACATTAGGCCTTCACTCACTGGGAAGTTTGCTGGCAAT CCTTGGTATTATGGCAAAGTCACCAGGCACCAGGCAGAAATGGCATTAAATGAAAGAGGGCATGAAGGAG ACTTCCTCATTCGTGACAGTGAATCTTCGCCAAATGATTTCTCAGTATCACTAAAAGCACAAGGGAAAAA CAAGCATTTTAAAGTCCAGCTGAAAGAGACTGTTTACTGCATTGGGCAGCGGAAATTCAGCACCATGGAG GAACTTGTAGAACATTACAAAAAGGCACCGATCTTTACAAGTGAGCAAGGAGAAAAATTATATCTCGTCA AGCATTTGTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205875 representing NM_010878
Red=Cloning site Green=Tags(s) MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKN LKDTLGIGKVKRKPSVPDTASPADDSFVDPGERLYDLNMPAFVKFNYMAEREDELSLIKGTKVIVMEKCS DGWWRGSYNGQIGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELN FEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTIMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGN PWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTME ELVEHYKKAPIFTSEQGEKLYLVKHLS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010878 |
ORF Size | 1131 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010878.3, NP_035008.2 |
RefSeq Size | 1699 bp |
RefSeq ORF | 1134 bp |
Locus ID | 17973 |
UniProt ID | Q99M51 |
Gene Summary | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209014 | Nck1 (untagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1), (10ug) |
CNY 3,990.00 |
|
MR205875 | Nck1 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 3,656.00 |
|
MR205875L3 | Lenti ORF clone of Nck1 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 4,750.00 |
|
MR205875L4 | Lenti ORF clone of Nck1 (mGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 4,750.00 |