Spop (BC043131) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TEF2, Pcif1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205802 representing BC043131 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAGGGTTCCAAGTCCTCCACCTCCGGCAGAAATGTCGAGTGGTCCCGTTGCCGAGAGCTGGTGCT ATACACAGATCAAGGTAGTGAAGTTCTCCTACATGTGGACCATCAATAACTTTAGTTTTTGCCGGGAGGA AATGGGTGAAGTCATAAAAAGTTCAACTTTTTCATCAGGAGCCAATGATAAACTGAAATGGTGTTTGCGA GTAAACCCGAAAGGGCTAGATGAAGAAAGCAAAGATTACCTGTCGCTTTACCTGTTGTTGGTCAGCTGTC CAAAGAGTGAAGTTCGGGCAAAGTTCAAGTTCTCCATCCTGAATGCCAAGGGAGAAGAAACCAAAGCTAT GGAGAGTCAGCGAGCTTATAGGTTCGTGCAAGGCAAAGACTGGGGATTCAAAAAATTCATCCGCAGAGAT TTTCTCTTGGACGAGGCCAATGGGCTTCTCCCTGATGACAAGCTCACCCTCTTCTGTGAGGTGAGTGTAG TACAAGACTCTGTCAATATTTCTGGGCAGAATACTATGAACATGGTAAAGGTTCCTGAATGCCGGTTGGC AGATGAGTTAGGAGGGCTGTGGGAGAACTCTCGATTCACCGACTGCTGCTTGTGTGTGGCTGGTCAAGAA TTCCAGGCTCACAAGGCTATCTTAGCAGCTCGGTCTCCAGTCTTCAGTGCCATGTTTGAACATGAAATGG AGGAGAGCAAAAAGAATCGGGTTGAGATTAACGACGTGGAGCCTGAGGTGTTTAAGGAGATGATGTGCTT CATCTACACGGGGAAGGCGCCGAACCTCGACAAGATGGCTGACGACTTGCTGGCAGCTGCCGATAAGTAC GCCCTGGAGCGTCTGAAGGTCATGTGTGAGGACGCCCTCTGCAGTAACCTCTCTGTGGAGAACGCTGCAG AGATTCTCATCCTGGCTGACCTCCACAGCGCAGATCAGCTGAAGACGCAGGCAGTTGATTTCATCAACTA TCATGCTTCGGATGTCTTGGAGACCTCTGGGTGGAAGTCAATGGTGGTGTCACATCCCCACTTAGTGGCA GAAGCATACCGTTCTCTGGCTTCAGCACAGTGCCCTTTCCTGGGGCCCCCACGCAAACGCCTGAAGCAAT CC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205802 representing BC043131 Red=Cloning site Green=Tags(s) MSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLR VNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRD FLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQE FQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKY ALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVA EAYRSLASAQCPFLGPPRKRLKQS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC043131
ORF Size	1122 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043131, AAH43131
RefSeq Size	2533 bp
RefSeq ORF	1124 bp
Locus ID	20747
Gene Summary	Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL (By similarity). In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation.[UniProtKB/Swiss-Prot Function]

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