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Camk1 (NM_133926) Mouse Tagged ORF Clone
CAT＃: MG205800
- TrueORF®
Camk1 (tGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase I (Camk1)
ORF Plasmid: DDK
"NM_133926" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI505105; CaMKIalpha; D6Ertd263e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205800 representing NM_133926
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAAGACATTAGGGATATTTATGACTTCAGAG ATGTTCTGGGCACGGGTGCCTTCTCAGAAGTGATCCTGGCAGAGGACAAGAGGACTCAGAAACTGGTGGC CATCAAATGCATTGCCAAGAAAGCCCTGGAGGGCAAAGAAGGCAGCATGGAGAACGAGATTGCCGTCTTA CACAAGATCAAGCACCCCAACATTGTAGCCCTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCA TCATGCAGCTGGTTTCAGGTGGAGAGCTGTTTGACCGAATTGTGGAGAAAGGATTCTACACGGAACGGGA TGCCAGCCGCCTCATCTTCCAGGTGCTGGATGCTGTCAAGTACCTGCACGACCTGGGCATTGTGCACCGG GATCTCAAGCCAGAGAACCTGCTGTACTACAGTCTGGATGAAGACTCCAAAATCATGATCTCTGACTTTG GCCTCTCCAAGATGGAGGACCCAGGCAGTGTGCTCTCCACAGCCTGTGGGACTCCAGGATATGTGGCCCC TGAGGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGATTGCTGGTCCATAGGAGTCATTGCCTATATA CTGCTCTGTGGCTACCCACCCTTTTATGATGAAAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCAG AGTATGAGTTTGACTCTCCTTATTGGGACGACATCTCTGACTCTGCCAAAGATTTCATACGACATTTGAT GGAGAAAGACCCGGAGAAGAGGTTTACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA GCTCTGGATAAGAATATCCACCAGTCAGTGAGCGAGCAGATCAAGAAGAACTTTGCCAAGAGCAAGTGGA AGCAAGCTTTCAATGCCACTGCTGTGGTTCGGCACATGAGGAAGCTGCAGCTGGGAACCAGTCAGGAGGG GCAGGGGCAGACTGGCAGCCACGGGGAGCTGCTGACACCAACAGCTGGTGGGCCAGCAGCTGGCTGCTGC TGCCGAGACTGCTGTGTGGAACCAGGCTCGGAACTGCCCCCTGCACCACCCCCAAGCTCTAGGGCCATGG AT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205800 representing NM_133926
Red=Cloning site Green=Tags(s) MPGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKKALEGKEGSMENEIAVL HKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHR DLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYI LLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDT ALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTGSHGELLTPTAGGPAAGCC CRDCCVEPGSELPPAPPPSSRAMD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133926 |
| ORF Size | 1122 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133926.2, NP_598687.1 |
| RefSeq Size | 1434 bp |
| RefSeq ORF | 1125 bp |
| Locus ID | 52163 |
| UniProt ID | Q91YS8 |
| Gene Summary | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-673', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation. Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200967 | Camk1 (untagged) - Mouse calcium/calmodulin-dependent protein kinase I (Camk1), (10ug) |
CNY 3,656.00 |
|
| MR205800 | Camk1 (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase I (Camk1) |
CNY 3,656.00 |
|
| MR205800L3 | Lenti ORF clone of Camk1 (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase I (Camk1) |
CNY 4,750.00 |
|
| MR205800L4 | Lenti ORF clone of Camk1 (mGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase I (Camk1) |
CNY 4,750.00 |
