Hsd3b1 (NM_008293) Mouse Tagged ORF Clone
CAT#: MG205766
- TrueORF®
Hsd3b1 (tGFP-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1)
ORF Plasmid: DDK
"NM_008293" in other vectors (4)
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CNY 3,140.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 3-beta-HSD I; D3Ertd383e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205766 representing NM_008293
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGATGGAGCTGCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCAAGATGTTGG TGCAGGAGAAAGAACTGCAGGAGGTCAGAGCTCTGGACAAAGTATTCCGACCAGAAACCAAGGAGGAATT CTCCAAGCTGCAGACAAAGACCAAGGTGACAGTGTTGGAAGGAGACATTCTGGATGCCCAGTGCCTGAGG AGAGCCTGCCAGGGCATCTCTGTTGTCATCCACACTGCTGCTGTCATTGATGTCACAGGTGTCATTCCCA GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACCTATTGGAGGCCTGTGTTCAAGCAAGTGT GCCAGCCTTCATCTTCTGCAGCTCAGTTGATGCTGCAGGGCCCAACTCGTACAAGAAGATCGTCCTGAAT GGCCACGAGGAACAGAATCATGAAAGCACATGGTCTGATCCATACCCATACAGCAAAAAGATGGCTGAGA AGGCAGTACTGGCAGCCAATGGGAGCATGCTGAAAAATGGTGGCACTTTGAATACTTGTGCCTTAAGACC CATGTACATTTATGGGGAGAGAAGTCCATTCATTTTTAATGCAATAATTAGGGCCCTCAAAAATAAGGGT ATTCTGTGTGTTACTGGCAAATTCTCCATAGCCAACCCAGTATATGTGGAAAATGTGGCCTGGGCACACA TTCTGGCAGCCAGGGGCCTTCGAGACCCCAAGAAGTCTACAAGCATCCAAGGACAGTTCTACTACATCTC AGATGACACCCCTCACCAAAGCTATGATGATTTAAATTACACCCTGAGTAAGGAATGGGGGCTCCGCCCT AATGCCAGCTGGAGCCTTCCTCTGCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC TGCTACGTCCAGTCTACAGGTATAGACCTCTCTTTAACCGCCACTCGATCACACTGTCAAATAGCACGTT CACTTTTTCTTACAAGAAAGCTCAGCGAGATCTGGGCTATGAGCCACTTGTCAACTGGGAGGAAGCAAAG CAGAAAACCTCAGAGTGGATAGGGACAATAGTGGAACAGCACAGGGAGATATTGGACACAAAGTGCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205766 representing NM_008293
Red=Cloning site Green=Tags(s) MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLR RACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDAAGPNSYKKIVLN GHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKG ILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRP NASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHSITLSNSTFTFSYKKAQRDLGYEPLVNWEEAK QKTSEWIGTIVEQHREILDTKCQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008293 |
ORF Size | 1119 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008293.2, NP_032319.1 |
RefSeq Size | 1853 bp |
RefSeq ORF | 1122 bp |
Locus ID | 15492 |
UniProt ID | P24815 |
Gene Summary | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3-beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201890 | Hsd3b1 (untagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10ug) |
CNY 3,656.00 |
|
MR205766 | Hsd3b1 (Myc-DDK-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1) |
CNY 3,656.00 |
|
MR205766L3 | Lenti ORF clone of Hsd3b1 (Myc-DDK-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1) |
CNY 4,750.00 |
|
MR205766L4 | Lenti ORF clone of Hsd3b1 (mGFP-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1) |
CNY 4,750.00 |