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Exo5 (NM_028457) Mouse Tagged ORF Clone
CAT＃: MG205764
- TrueORF®
Exo5 (tGFP-tagged) - Mouse RIKEN cDNA 3110037I16 gene (3110037I16Rik)
"NM_028457" in other vectors (5)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110037I16Rik; AV297100; Dem1; Exo V; mExo5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205764 representing NM_028457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGACTGGGGAAGAGGAGACAGCATCAGCAGAAGCCTCAGGGTTTTCAGACTTGAGTGACTCAG AGTTAGTTGAATTTCTGGATCTGGAAGAAGCCAAAGAATCGGCTGTTTCACTTAGCAAGCCTGGCCCTTC TGCTGAACTCCCTGGGAAGGATGACAAACCAGTAAGCTTGCAGAACTGGAAAGGGGGATTGGATGTCTTG TCACCCATGGAGAGATTCCACCTGAAATATTTATATGTCACTGACCTGTGCACTCAGAACTGGTGTGAGT TGCAGATGGTGTACGGGAAGGAGCTTCCTGGTTCGTTGACACCTGAGAAAGCAGCTGTTTTGGACACTGG TGCTAGCATCCACCTAGCAAAAGAACTAGAACTTCATGACCTTGTGACAGTCCCCATCGCCACAAAAGAA GATGCTTGGGCAGTTAAGTTTCTGAACATACTAGCAATGATTCCTGCCCTACAGTCGGAAGGGCGCGTCA GAGAGTTTCCAGTGTTTGGGGAGGTGGAGGGAATATTTCTTGTTGGAGTCATTGATGAGTTGCACTACAC ATCCAAGGGGGAACTAGAGCTGGCTGAACTCAAGACACGAAGGCGCCCCGTGCTCCCCCTGCCAGCTCAG AAAAAGAAAGACTATTTTCAAGTTAGCCTATACAAATATATCTTTGATGCCATGGTACAAGGGAAAGTGA CTCCTGCTAGCCTAATCCACCACACTAAATTGTGTCTAGACAAGCCACTGGGACCTTCTGTGCTGAGGCA TGCCAGACAAGGAGGCGTGTCTGTAAAATCTTTGGGTGACCTTATGGAACTGGTTTTCTTGTCTCTTACA CTGTCTGATCTCCCAGCTATTGATACCCTAAAACTTGAGTATATCCATCAAGAGACTGCCACTATACTGG GCACAGAGATTGTAGCCTTTGAAGAGAAGGAAGTGAAAAGCAAGGTGCAGCATTACGTGGCCTACTGGAT GGGCCACCGAGATCCTCAAGGCGTTGATGTGGAGGAGGCATGGAAGTGCCGGACCTGTGACTATGTGGAC ATCTGCGAGTGGAGGAGGGGCAGTGGAGTGCTCAGCTCATCGTGGGAGCCCAAAGCCAAGAAGTTTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205764 representing NM_028457
Red=Cloning site Green=Tags(s) MAETGEEETASAEASGFSDLSDSELVEFLDLEEAKESAVSLSKPGPSAELPGKDDKPVSLQNWKGGLDVL SPMERFHLKYLYVTDLCTQNWCELQMVYGKELPGSLTPEKAAVLDTGASIHLAKELELHDLVTVPIATKE DAWAVKFLNILAMIPALQSEGRVREFPVFGEVEGIFLVGVIDELHYTSKGELELAELKTRRRPVLPLPAQ KKKDYFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLDKPLGPSVLRHARQGGVSVKSLGDLMELVFLSLT LSDLPAIDTLKLEYIHQETATILGTEIVAFEEKEVKSKVQHYVAYWMGHRDPQGVDVEEAWKCRTCDYVD ICEWRRGSGVLSSSWEPKAKKFK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028457 |
| ORF Size | 1987 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028457.2, NP_082733.1 |
| RefSeq Size | 1988 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 73172 |
| UniProt ID | Q9CXP9 |
| Gene Summary | Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201633 | Exo5 (untagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
| MG217153 | Exo5 (tGFP-tagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1) transcript variant 1, (10ug) |
CNY 3,140.00 |
|
| MR217153 | Exo5 (Myc-DDK-tagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1), transcript variant 1 |
CNY 3,656.00 |
|
| MR217153L3 | Lenti ORF clone of Exo5 (Myc-DDK-tagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1), transcript variant 1 |
CNY 4,750.00 |
|
| MR217153L4 | Lenti ORF clone of Exo5 (mGFP-tagged) - Mouse defects in morphology 1 homolog (S. cerevisiae) (Dem1), transcript variant 1 |
CNY 4,750.00 |
