Dapk2 (BC022165) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205712 representing BC022165 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCAGGCCTCGATGAGGAGCCCAAATATGGAGACGTTCAAACAGCAGAAGGTGGAGGACTTTTATG ACATCGGAGAGGAGCTGGGCAGTGGCCAGTTTGCCATCGTGAAGAAGTGCCGGGAGAAGAGCACAGGGCT GGAGTATGCAGCCAAGTTCATTAAGAAGAGGCAGAGCCGGGCCAGCCGTCGGGGCGTGTGCCGGGAGGAA ATCGAGCGGGAGGTGAGCATCCTGCGGCAGGTGCTGCACCCCAACATCATCACGCTGCACGACGTCTATG AGAACCGCACCGACGTGGTGCTCATCCTTGAGCTAGTGTCCGGAGGAGAACTGTTTGATTTCCTGGCCCA GAAGGAGTCGTTAAGTGAGGAGGAAGCCACCAGCTTCATTAAGCAGATCCTGGATGGGGTGAATTACCTT CACACAAAGAAAATTGCTCACTTTGATCTCAAGCCAGAAAACATCATGTTGTTAGACAAGAATATCCCCA TTCCACACATCAAGCTGATTGACTTTGGCCTGGCTCACGAAATAGAAGATGGAGTTGAATTTAAAAACAT TTTTGGGACACCTGAATTTGTTGCTCCAGAAATCGTGAACTATGAGCCACTGGGACTGGAGGCCGACATG TGGAGCATTGGAGTCATCACCTACATCCTTCTAAGTGGAGCGTCCCCCTTCCTGGGAGACACAAAACAAG AAACCCTGGCAAATATCACTGCTGTGAGTTACGACTTTGATGAGGAATTCTTCAGCCAGACAAGCGAGCT GGCCAAGGACTTCATTCGGAAGCTTCTTGTGAAAGAGACCCGGAAACGGCTTACCATCCAAGAGGCTCTC AGACATCCCTGGATCACGCCGGTGGACACCCAGCAAGCTATGGTACGCAGAGAGTCCGTGGTCAACCTGG AGAATTTTAAGAAGCAGTATGTCCGCAGGCGGTGGAAGCTGTCCTTCAGCATCGTCTCCTTGTGCAACCA CCTCACTCGCTCCCTGATGAAGAAGGTACATCTGAGGACAAGCGAGGACCTGAGGAACTGCGAGAGTGAC ACAGAGGAGAACATCGCCAGGAGGAAAGCCCTTCACCCCCGGAGGAGGAGCAGTACCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205712 representing BC022165 Red=Cloning site Green=Tags(s) MVQASMRSPNMETFKQQKVEDFYDIGEELGSGQFAIVKKCREKSTGLEYAAKFIKKRQSRASRRGVCREE IEREVSILRQVLHPNIITLHDVYENRTDVVLILELVSGGELFDFLAQKESLSEEEATSFIKQILDGVNYL HTKKIAHFDLKPENIMLLDKNIPIPHIKLIDFGLAHEIEDGVEFKNIFGTPEFVAPEIVNYEPLGLEADM WSIGVITYILLSGASPFLGDTKQETLANITAVSYDFDEEFFSQTSELAKDFIRKLLVKETRKRLTIQEAL RHPWITPVDTQQAMVRRESVVNLENFKKQYVRRRWKLSFSIVSLCNHLTRSLMKKVHLRTSEDLRNCESD TEENIARRKALHPRRRSSTS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC022165
ORF Size	1112 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC022165, AAH22165
RefSeq Size	1883 bp
RefSeq ORF	1112 bp
Locus ID	13143
Gene Summary	Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Capable of regulating both type I apoptotic and type II autophagic cell death signals. The former involves caspase activation, chromatin and mitochondrial condensation while the latter involves caspase-independent cell death in conjunction with accumulation of mature autophagic vesicles, plasma membrane blebs, and nuclear condensation without DNA degradation. Mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (By similarity). Regulates granulocytes motility by controlling cell spreading and polarization (PubMed:24163421).[UniProtKB/Swiss-Prot Function]

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