Mapk13 (BC001992) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SAPK4; Serk4
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205630 representing BC001992 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTCACTCGGAAAAGGGGCTTCTACAAGCAGGACATCAACAAGACTGCCTGGGAGCTACCCAAGA CCTACCTGGCGCCCGCGCACGTCGGCAGCGGGGCCTATGGAGCGGTGTGCTCGGCCATCGACAAGCGGAC AGGGGAGAAGGTGGCCATCAAGAAGCTGAGCCGGCCCTTCCAGTCGGAGATCTTTGCCAAGCGCGCTTAC CGCGAGCTTCTGCTCTTGAAGCACATGCACCATGAGAACGTCATTGGGCTTCTGGATGTCTTCACCCCTG CGTCTTCCCTTCGGAGCTTCCATGATTTCTACCTGGTGATGCCCTTCATGCAGACAGACCTACAGAAGAT CATGGGGATGGAATTCAGCGAGGATAAGGTCCAGTACTTGGTGTACCAGATGCTCAAAGGTCTAAAGTAC ATCCACTCCGCTGGCATCGTCCACAGGGACCTGAAACCGGGCAACCTGGCTGTGAATGAAGACTGTGAGC TGAAGATCCTGGACTTTGGGCTGGCCCGCCACACAGACACTGAGATGACGGGCTATGTGGTGACCCGCTG GTACCGGGCCCCCGAGGTGATCCTCAGCTGGATGCATTACAACCAGACAGTCGACATCTGGTCTGTTGGT TGCATCATGGCAGAAATGCTGACTGGAAAGACACTCTTCAAGGGCAAGGACTACCTGGACCAGCTGACCC AGATCCTGAAAGTGACTGGGGTGCCAGGTGCCGAGTTCGTGCAGAAGCTGAAAGACAAGGCGGCCAAATC CTATATTCAGTCCCTGCCCCAGAGCCCCAAGAAGGATTTCACACAGCTCTTTCCACGCGCCAGTCCGCAA GCTGCAGACCTGCTCGACAAGATGCTGGAGCTGGATGTGGACAAGCGTCTGACCGCTGCTCAGGCACTGG CTCACCCCTTCTTTGAACCCTTCCGGGACCCTGAGGAGGAGACAGAGGCCCAGCAGCCTTTTGATGATGC CTTAGAACATGAGAAACTCAGTGTGGACGAATGGAAACAACACATCTACAAAGAGATCTCAAACTTCAGT CCCATAGCCCGGAAGGACTCACGGCGACGAAGTGGCATGAAGCTGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205630 representing BC001992 Red=Cloning site Green=Tags(s) MSLTRKRGFYKQDINKTAWELPKTYLAPAHVGSGAYGAVCSAIDKRTGEKVAIKKLSRPFQSEIFAKRAY RELLLLKHMHHENVIGLLDVFTPASSLRSFHDFYLVMPFMQTDLQKIMGMEFSEDKVQYLVYQMLKGLKY IHSAGIVHRDLKPGNLAVNEDCELKILDFGLARHTDTEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVG CIMAEMLTGKTLFKGKDYLDQLTQILKVTGVPGAEFVQKLKDKAAKSYIQSLPQSPKKDFTQLFPRASPQ AADLLDKMLELDVDKRLTAAQALAHPFFEPFRDPEEETEAQQPFDDALEHEKLSVDEWKQHIYKEISNFS PIARKDSRRRSGMKLQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC001992
ORF Size	1100 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001992, AAH01992
RefSeq Size	1376 bp
RefSeq ORF	1100 bp
Locus ID	26415
Gene Summary	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.[UniProtKB/Swiss-Prot Function]

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