Setd7 (NM_080793) Mouse Tagged ORF Clone
CAT#: MG205628
- TrueORF®
Setd7 (tGFP-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7)
ORF Plasmid: DDK
"NM_080793" in other vectors (3)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1600028F23Rik; H3K4MT; KMT7; mKIAA1717; Set7; Set7/9 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205628 representing NM_080793
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGCGACGATGAGGTGGTGGAGGAAGCCGTGGAAGGGCACCTGGACGATGACGGATTACCACACG GGTTCTGCACTGTCACCTACTCCTCCACGGACAGATTTGAGGGCAACTTTGTGCATGGAGAAAAGAATGG GCGTGGGAAATTCTTCTTCTTTGACGGCAGCACCCTGGAGGGATATTACGTGGACGATGCCCTGCAGGGC CAGGGCGTTTACACCTATGTGGATGGAGGTGTTCTCCAAGGCACCTATGTGGATGGAGAGCTGAATGGAC CGGCCCAGGAGTACGACTCTGACGGGAGGCTGATCTTCAAGGGACAGTACAAAGACAACAATCGACACGG AGTGTGTTGGATCCATTACCCGGATGGAGGCAGCCTCGTCGGGGAAGTCAATGAAGATGGAGAGATGACG GGAGAAAAGATCGCCTATGTGTACCCTGACCAGAGGACTGCGCTCTATGGGAAGTTCATTGACGGAGAGA TGCTCGAAGGGAAACTGGCCACCCTCATGGCCACCGAAGAAGGGAGGCCACACTTTGAAGTGACGTCGGG AAGTTCCGTGTACCACTTTGATAAATCGACTTCCTCCTGCATCTCCAGCGATGCTCTCCTTCCAGACCCT TATGAGTCTGAGAGGGTTTATGTTGCCGACTCTCTCATCTCCAGTGCTGGAGAAGGGCTGTTTTCCAAGG TAGCAGTTGGACCTAATACTGTTATGTCGTTTTATAATGGAGTCCGAATTACACACCAAGAGGTTGACAG CAGGGATTGGGCCCTAAATGGGAACACTCTCTCCCTGGACGAGGAGACAGTCATTGATGTGCCCGAGCCC TACAACCACGTGTCCAAGTACTGTGCCTCCTTGGGACACAAGGCCAATCACTCCTTCACTCCGAACTGCG TCTATGACCTGTTTGTCCACCCCCGTTTCGGGCCGATCAAGTGCATCCGCACCCTGCGAGCTGTGGAGGC CGAGGAAGAGCTGACCGTTGCCTACGGCTATGACCACAGCCCCCCGGGGAAGAGTGGGCCTGAAGCCCCC GAGTGGTATCAAGTGGAGCTGAAGGCCTTCCAGGCCACCCAGCAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205628 representing NM_080793
Red=Cloning site Green=Tags(s) MDSDDEVVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQG QGVYTYVDGGVLQGTYVDGELNGPAQEYDSDGRLIFKGQYKDNNRHGVCWIHYPDGGSLVGEVNEDGEMT GEKIAYVYPDQRTALYGKFIDGEMLEGKLATLMATEEGRPHFEVTSGSSVYHFDKSTSSCISSDALLPDP YESERVYVADSLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEP YNHVSKYCASLGHKANHSFTPNCVYDLFVHPRFGPIKCIRTLRAVEAEEELTVAYGYDHSPPGKSGPEAP EWYQVELKAFQATQQK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080793 |
ORF Size | 1098 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080793.2 |
RefSeq Size | 7357 bp |
RefSeq ORF | 1101 bp |
Locus ID | 73251 |
UniProt ID | Q8VHL1 |
Gene Summary | Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR205628 | Setd7 (Myc-DDK-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7) |
CNY 3,656.00 |
|
MR205628L3 | Lenti ORF clone of Setd7 (Myc-DDK-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7) |
CNY 4,750.00 |
|
MR205628L4 | Lenti ORF clone of Setd7 (mGFP-tagged) - Mouse SET domain containing (lysine methyltransferase) 7 (Setd7) |
CNY 4,750.00 |