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Pola2 (BC006945) Mouse Tagged ORF Clone
CAT＃: MG205518
- TrueORF®
Pola2 (tGFP-tagged) - Mouse polymerase (DNA directed), alpha 2 (cDNA clone MGC:11533 IMAGE:3602559)
ORF Plasmid: DDK
"BC006945" in other vectors (3)
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CNY 2,280.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI573378 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205518 representing BC006945
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGTCCACCCAGCAGCTGGCGGAGGAACTACAGATCTTCGGCCTGGACTACGAGGACTCCCTGT TGGAGAAACTGGCAGAGCTGTGTGTTCTATACCGACAGACTGAGGACGGGATGGTCAGTGAGCTCATCGC CTTCTGCACCAGTGCGGGGAAGACATGCCTTACTGTAGACATCCTGAACTCTTTTGAATACGAGGTTCTG AACAAAAAACTCTCCAAAGCCTGGCACAGTGCCTCCAAAGACAGTGGGCATGCAGGAACCAGAGACATTG TTTCTATACAAGAGCTAATTGAAGCAGAGGAGGAAGAGGAGACCCTCCTAAGCTCTTATACCACACCCTC TAAGGGTCCTCTCAAGCGAGTTAGCAGCACCCCAGAAACCCCCCTAACCAAAAGGAGCGTCGCTGCTCGC AGCCCACGTCAGCTTCTCTCACCGTCAAGTTTCTCCCCGAGTGCTACCCCCTCCCAGAAATACACCTCCA GAACCAACCGGGGAGAAGTGGTGACCACCTTTGGCTCCGCACAGGGCTTGTCTTGGTCCGGGAGAGGAGG CTCTGGAAGCGTCAGTCTAAAGGTCGTGGGGGACCCAGAGCCACTCACTGGCAGCTACAAAGCGATGTTT CAGCAGCTCATGGGTGTTCGAGAAGTTCTGACCAGTAAGATCGAAGAGCTCGGCAGCGAGCTGAAGGAGC ATCACAAGATCGAGGCCTTTACCCCTCTCCTGGTCCCAGCGCAAGAGCCTGTTATTCTCCTGGGTCAGAT TGGCTGCGACAGCAACGGGAAGCTGAACAGCAAGTCCGTGATTCTCGAGGGGGACCAGGAGCACTCCTAT GGCGCGCAGATCCCAGTGGACCTATCGGAACTCAAAGAGTATTCCCTCTTCCCTGGACAGGTTGTAATTA TGGAAGGATTCAACACCACTGGTAGGAGACTTACTGCCACCAAACTCTATGAGGTACAGGGTGGGCCTCC CCTCCCCCACTCCACATTGGCTTCAGGCTCAGTATGTTCAGTTCTGGTGAAAGATGCTGGTCCTTTTCAG TCTCTGTCATTAGCTAGGCTTTTCTGTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205518 representing BC006945
Red=Cloning site Green=Tags(s) MAVSTQQLAEELQIFGLDYEDSLLEKLAELCVLYRQTEDGMVSELIAFCTSAGKTCLTVDILNSFEYEVL NKKLSKAWHSASKDSGHAGTRDIVSIQELIEAEEEEETLLSSYTTPSKGPLKRVSSTPETPLTKRSVAAR SPRQLLSPSSFSPSATPSQKYTSRTNRGEVVTTFGSAQGLSWSGRGGSGSVSLKVVGDPEPLTGSYKAMF QQLMGVREVLTSKIEELGSELKEHHKIEAFTPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQEHSY GAQIPVDLSELKEYSLFPGQVVIMEGFNTTGRRLTATKLYEVQGGPPLPHSTLASGSVCSVLVKDAGPFQ SLSLARLFCF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC006945 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006945, AAH06945 |
| RefSeq Size | 2514 bp |
| RefSeq ORF | 1082 bp |
| Locus ID | 18969 |
| Gene Summary | May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR205518 | Pola2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 2 (cDNA clone MGC:11533 IMAGE:3602559) |
CNY 3,656.00 |
|
| MR205518L3 | Lenti ORF clone of Pola2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), alpha 2 (cDNA clone MGC:11533 IMAGE:3602559) |
CNY 3,990.00 |
|
| MR205518L4 | Lenti ORF clone of Pola2 (mGFP-tagged) - Mouse polymerase (DNA directed), alpha 2 (cDNA clone MGC:11533 IMAGE:3602559) |
CNY 3,990.00 |
