Eci2 (NM_011868) Mouse Tagged ORF Clone

CAT#: MG205475

  • TrueORF®

Eci2 (tGFP-tagged) - Mouse peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase (Peci)



  "NM_011868" in other vectors (5)

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Mouse Protein / Lysate Request

CNY 2,280.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Acbd2; Drs1; Hca88; Peci
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205475 representing NM_011868
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGCCAGTCAGCAGGACTTTGAAAATGCATTGAACCAGGTGAAACTCTTGAAGAAGGACCCGGGAA
ACGAAGTGAAGCTAAGACTCTATGCACTGTACAAGCAGGCCACAGAAGGACCCTGTAATATGCCTAAGCC
TGGTATGCTTGACTTTGTCAACAAAGCCAAGTGGGATGCATGGAATGCCCTGGGCAGCCTACCCAAGGAA
ACTGCCAGGCAGAACTATGTGGATCTCGTATCCAGTTTGAGTTCCTCATCTGAAGCCCCGAGCCAGGGAA
AGTGTGGAGCTGATGAGAAAGCCCAGGAGTCCAAAGACATCCTGGTAACTTCTGAAGATGGCATCACGAA
GATCACGTTCAATCGGCCCACCAAAAAGAATGCCATATCCTTCCAGATGTATCGGGATATTATACTCGCA
CTTAAAAATGCCAGCACTGATAACACTGTCATGGCTGTTTTCACAGGAACTGGTGACTACTACTGCAGTG
GGAATGACCTGACTAACTTCACTAGTGCCACTGGGGGGATAGAGGAGGCAGCTAGCAACGGTGCTGTGTT
ACTGAGGGACTTTGTAAACAGTTTTATAGACTTTCCCAAGCCTCTGGTTGCGGTAGTAAATGGCCCAGCT
GTGGGAATCTCTGTCACCCTTCTGGGACTATTTGATGCTGTCTTTGCATCAGACAGGGCAACGTTTCATA
CTCCATTCAGCCAACTCGGCCAGAGCCCAGAAGCATGCTCCTCTTACACGTTTCCGAAGATGATGGGCTC
AGCAAAGGCAGCTGAGATGCTTCTCTTTGGGAAGAAGCTCACAGCAAGAGAGGCCTGGGCTCAAGGCCTT
GTCACTGAAGTTTTCCCTGAGAGCACTTTTGAGACGGAAGTCTGGACCAGGCTGAAAACATATGCGAAGC
TGCCGCCAAACGCCATGAGAATTTCCAAAGAGTTAATCAGAAAAAATGAGAAAGAAAAGCTCTACGCAGT
CAACGCTGAAGAGTGCACTACTCTGCAAGCAAGGTGGCTGTCAGAGGAGTGCATGAACGCAATCATGAGC
TTCGTCTCCAGAAAACCAAAGCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205475 representing NM_011868
Red=Cloning site Green=Tags(s)

MRASQQDFENALNQVKLLKKDPGNEVKLRLYALYKQATEGPCNMPKPGMLDFVNKAKWDAWNALGSLPKE
TARQNYVDLVSSLSSSSEAPSQGKCGADEKAQESKDILVTSEDGITKITFNRPTKKNAISFQMYRDIILA
LKNASTDNTVMAVFTGTGDYYCSGNDLTNFTSATGGIEEAASNGAVLLRDFVNSFIDFPKPLVAVVNGPA
VGISVTLLGLFDAVFASDRATFHTPFSQLGQSPEACSSYTFPKMMGSAKAAEMLLFGKKLTAREAWAQGL
VTEVFPESTFETEVWTRLKTYAKLPPNAMRISKELIRKNEKEKLYAVNAEECTTLQARWLSEECMNAIMS
FVSRKPKL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011868
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011868.1
RefSeq Size 1581 bp
RefSeq ORF 1077 bp
Locus ID 23986
UniProt ID Q9WUR2
Gene Summary Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (PubMed:24344334). Has a preference for 3-trans substrates (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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