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Necab1 (NM_178617) Mouse Tagged ORF Clone
CAT＃: MG205347
- TrueORF®
Necab1 (tGFP-tagged) - Mouse EF hand calcium binding protein 1 (Efcbp1)
ORF Plasmid: DDK
"NM_178617" in other vectors (4)
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CNY 3,140.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700003H21Rik; Efcbp1; STIP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205347 representing NM_178617
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCCCGGGAGACTTCGCCTTCCTCCAACAACTCCTCGGAAGAGCTCAGCTCTACCCTGCAAC TGTCCAAGGGCATGTCCATCTTCCTCGACATTCTGAGGAGAGCAGACAAAAATGATGATGGAAAATTGTC CTTTGAGGAATTTAAAGCATATTTTGCAGATGGAGTTCTCAGTGGAGAAGAACTACATGAGCTCTTCCAC ACCATTGACACACACAATACCAACAATCTCGACACAGAAGAACTATGCGAATATTTTTCTCAGCACTTGG GTGAATATGAGAATGTACTGGCCGCACTTGAAGACCTGAATCTCTCCATCCTGAAGGCAATGGGCAAGAC AAAGAAAGACTACCAAGAAGCTTCCAATTTGGAACAATTTGTCACTCGATTTTTGTTGAAGGAGACCTTG AATCAGTTGCAGTCTCTCCAGAACTCTCTGGAATGTGCCATGGAAACTACTGAAGAGCAAACGCGTCAAG AAAGGCAAGGACCATCCAAGCCAGAAGTCCTGTCCATTCAATGGCCTGGAAAGCGATCCAGCCGCCGAGT CCAGAGACACAATAGTTTCTCCCCCAACAGCCCGCAGTTCAATGTCAGCAGTCCAGCATTGTTAGAAGAA GACAACCAGTGGATGACCCAGATAAACAGACTCCAGAAATTAATTGATAGACTGGAAAAGAAGGATCTGA AACTTGAACCCCTGGAAGAAGAAATTATTGAAGAGAACACTAAACCTCACATCATGCTTGTGCAGCGCCA GATGTCTGTGACAGAAGAGGATCTGGAAGAATTCCAGCTTGCTCTGAAGCACTATGTGGAGAGTGCTTCA GCTCAAAGTGGATGCCTGCGTATCTCTATACAGAAGCTCTCAAACGAATCTCGCTACATGATTTATGAGT TCTGGGAGAACAGCAGTGTGTGGAACCGCCACCTCCAGACGAATTACAGCAAGACGTTCCAAAGAAGCAA CGTGGATTTCTTGGAAACTCCAGAGCTCACATCTACCATGCTAGTTCCTGCTTCATGGTGGATCCTGAAC AACAAT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205347 representing NM_178617
Red=Cloning site Green=Tags(s) MEDSRETSPSSNNSSEELSSTLQLSKGMSIFLDILRRADKNDDGKLSFEEFKAYFADGVLSGEELHELFH TIDTHNTNNLDTEELCEYFSQHLGEYENVLAALEDLNLSILKAMGKTKKDYQEASNLEQFVTRFLLKETL NQLQSLQNSLECAMETTEEQTRQERQGPSKPEVLSIQWPGKRSSRRVQRHNSFSPNSPQFNVSSPALLEE DNQWMTQINRLQKLIDRLEKKDLKLEPLEEEIIEENTKPHIMLVQRQMSVTEEDLEEFQLALKHYVESAS AQSGCLRISIQKLSNESRYMIYEFWENSSVWNRHLQTNYSKTFQRSNVDFLETPELTSTMLVPASWWILN NN SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178617 |
| ORF Size | 1056 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178617.4, NP_848732.3 |
| RefSeq Size | 4919 bp |
| RefSeq ORF | 1059 bp |
| Locus ID | 69352 |
| UniProt ID | Q8BG18 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207682 | Necab1 (untagged) - Mouse N-terminal EF-hand calcium binding protein 1 (Necab1), (10ug) |
CNY 3,990.00 |
|
| MR205347 | Necab1 (Myc-DDK-tagged) - Mouse N-terminal EF-hand calcium binding protein 1 (Necab1) |
CNY 3,656.00 |
|
| MR205347L3 | Lenti ORF clone of Necab1 (Myc-DDK-tagged) - Mouse N-terminal EF-hand calcium binding protein 1 (Necab1) |
CNY 4,750.00 |
|
| MR205347L4 | Lenti ORF clone of Necab1 (mGFP-tagged) - Mouse N-terminal EF-hand calcium binding protein 1 (Necab1) |
CNY 4,750.00 |
