Zdhhc19 (BC049761) Mouse Tagged ORF Clone
CAT#: MG205230
- TrueORF®
Zdhhc19 (tGFP-tagged) - Mouse zinc finger, DHHC domain containing 19 (cDNA clone MGC:58833 IMAGE:6773873)
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CNY 3,140.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MGC58833 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205230 representing BC049761
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTCTTAAAGGATGCTGTGACACTTGTGAAGGAACCACAACAGCTGCCCTCCATCCCTCTTTCAT GGTTCCCCTCGAGCGTGTTTGCTGCCTTCAATGTAACGCTGCTGCTGTTTTTGAGTGGCCTTTTCTTCGG ATTCCCTTGTAGGTGGCTGGTTCAGAACGGGGAGTGGGCCTTTCCTGCCATCACAGGACCACTCTTCATC CTCACCTTCTTCAGTCTCGTCTCGCTCAACTTCTCAGACCCTGGTATCTTACATCGAGGCTCCACCAAAG AGGACCCCATGACGGTGCATGTTGTTCGAGTGAACCAAAGGGCTTTCCGCCTGGAATGGTGCCCCAAGTG CCTCTTCCATCGCCCACCCCGAACCTACCACTGCCCGTGGTGCAACATTTGCGTGGAGGACTTCGACCAC CATTGCAAGTGGGTCAATAACTGCATCGGTCACCGCAACTTCCGCCTCTTCATGCTGCTGGTCCTGTCAC TCTGTCTCTACTCGGGAGCCCTGCTGGTCACCTGCCTGACATTCCTATTTCGCACAAGGCATCTGCCCTT CTCCCTGGACAAGGGGATGGCCATCCTGGTGGCTGTGCCCGCTGCGGGCTTTTTGATTCCGCTCTTCCTG CTGCTGCTGATCCAGGCCCTATCTGTGAGCAGGGCGGAGAGCTCCTACGAGAGCAAGTGCAGGTACCATC CGGAATACAACCCTTTTGACCAGGGCTTTGCCAAGAACTGGTATCTGGCAATGTTTGCACCACTGGGTCC CAATTACATGTCTGAAGTTGTCTGTCTGCAAAGACCAGTGGGGACAGCATGGATCCAAGAGAAGACAAAG CCCTCACCACCACGTCGTCCTAAACACTGTAGGCCAGGTCCTCCGGGGCCCCAGCACCAACCTCGGCGCG TTCCAGGGAAGGGGCCCCCAGGGAGTGGAGAGGCTGCAGCTCTCCAAGAGATGCGAAGGCTGCCTGCATC TGTGGAAAAGTCCCCAGGAGGTCCACGCCAACCCACGGCTGAACCTGCCGCTGGAGACCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205230 representing BC049761
Red=Cloning site Green=Tags(s) MPFLKDAVTLVKEPQQLPSIPLSWFPSSVFAAFNVTLLLFLSGLFFGFPCRWLVQNGEWAFPAITGPLFI LTFFSLVSLNFSDPGILHRGSTKEDPMTVHVVRVNQRAFRLEWCPKCLFHRPPRTYHCPWCNICVEDFDH HCKWVNNCIGHRNFRLFMLLVLSLCLYSGALLVTCLTFLFRTRHLPFSLDKGMAILVAVPAAGFLIPLFL LLLIQALSVSRAESSYESKCRYHPEYNPFDQGFAKNWYLAMFAPLGPNYMSEVVCLQRPVGTAWIQEKTK PSPPRRPKHCRPGPPGPQHQPRRVPGKGPPGSGEAAALQEMRRLPASVEKSPGGPRQPTAEPAAGDP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC049761 |
ORF Size | 1041 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC049761, AAH49761 |
RefSeq Size | 1339 bp |
RefSeq ORF | 1043 bp |
Locus ID | 245308 |
Gene Summary | Palmitoyltransferase that mediates palmitoylation of STAT3 and RRAS. Palmitoylation of STAT3 induces the homodimerization and transcriptional activation of STAT3, thereby, promoting inflammation and cancer. Palmitoylation of RRAS is linked to cell viability. May be involved in S-stearoylation of STAT3.[UniProtKB/Swiss-Prot Function] |
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