Pard6a (BC049593) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Par6, TAX40, Par-6, Par6c, Tip-40, PAR6alpha
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205193 representing BC049593 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCCGCAGAGGACTCCGGCGCGCAGTCCCGATAGCATCGTCGAGGTGAAGAGCAAATTTGACG CCGAGTTCCGACGCTTTGCACTACCCCGCACTTCGGTGAGAGGCTTTCAGGAGTTCTCGCGATTGCTGTG TGTGGTACACCAGATCCCTGGCCTGGACGTCCTGCTTGGCTATACGGATGCTCACGGTGACTTGCTGCCC CTCACCAACGATGACAGTTTGCACCGGGCCCTGGCCAGCGGGCCCCCACCTCTGCGCCTCTTGGTTCAGA AACGGGAAGGTGACTCGAGTGGCCTGGCTTTTGCCTCCAACTCTCTACAAAGGCGCAAGAAAGGGCTCCT GCTACGACCAGTGGCACCTCTGCGCACCAGGCCACCCTTGTTAATCAGCTTGCCCCAAGATTTCCGCCAG GTGTCTTCAGTTATAGATGTGGACCTACTACCTGAGACCCACCGACGAGTGAGGCTGCACAAACATGGTT CAGACCGTCCCCTGGGCTTCTACATTCGAGATGGCATGAGTGTTCGCGTGGCTCCCCAGGGCCTGGAGCG GGTTCCAGGTATCTTCATCTCCCGCCTGGTACGTGGGGGCCTGGCTGAGAGTACAGGGCTGCTGGCGGTC AGTGATGAGATCCTTGAGGTCAACGGCATTGAGGTGGCCGGGAAGACCTTGGACCAAGTGACGGACATGA TGGTCGCCAACAGCCACAACCTCATCGTCACTGTCAAGCCTGCCAACCAGCGTAATAATGTGGTACGGGG GGCATCTGGGCGTCTGACAGGGCCTTCCTCTGTAGGGCCTGGGCCTACTGATCCTGACAGTGACGATGAC AGCAGTGACCTGGTCATTGAGAATCGCCACCCTCCCTGTTCTAATGGGCTGTCTCAGGGGCCCCTGTGCT GGGACCTGCAACCTGGCTGCCTACTTCCTGGTGCTGGCAGCTCTCTGCCCTCCTTGGATAGCAGAGAGCA AGCCAATTCTGGCTGGGGGAATGGCATGCGAGGTGATGTTAGCGGATTCAGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205193 representing BC049593 Red=Cloning site Green=Tags(s) MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRTSVRGFQEFSRLLCVVHQIPGLDVLLGYTDAHGDLLP LTNDDSLHRALASGPPPLRLLVQKREGDSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQ VSSVIDVDLLPETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAV SDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPSSVGPGPTDPDSDDD SSDLVIENRHPPCSNGLSQGPLCWDLQPGCLLPGAGSSLPSLDSREQANSGWGNGMRGDVSGFSL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC049593
ORF Size	1037 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049593, AAH49593
RefSeq Size	1271 bp
RefSeq ORF	1037 bp
Locus ID	56513
Gene Summary	Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:15761148). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (By similarity).[UniProtKB/Swiss-Prot Function]

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