Gmpr (BC010827) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2310004P21Rik; AV028449; GMPR 1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205188 representing BC010827 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCATAGACGCGGACCTTAAACTCGACTTCAAGGATGTTCTGCTGCGACCTAAGCGGAGCAGCC TCAAGAGCCGATCCGAGGTGGATCTTGAGCGAACTTTTACATTTCGAAACTCGAAACAAACCTACTCAGG GATTCCCATCATTGTGGCCAACATGGACACTGTGGGGACATTTGAGATGGCTGTGGTGATGTCACAGCAT GCCATGTTTACAGCCGTTCACAAGCACTACTCCCTGGATGACTGGAAATGTTTTGCGGAAACCCACCCCG AATGCCTGCAGCATGTAGCCGTGAGTTCTGGCAGCGGGCAGAATGATCTTGAGAGGATGAGCCGCATCTT GGAAGCTGTGCCTCAGGTGAAGTTCATCTGCCTGGATGTGGCCAATGGGTATTCAGAGCATTTTGTGGAA TTCGTGAAACTGGTCCGATCCAAATTCCCCGAACACACCATCATGGCAGGGAACGTGGTGACCGGAGAGA TGGTGGAAGAGCTCATCCTCTCTGGAGCAGATATCATCAAAGTGGGAGTCGGACCAGGTTCTGTGTGCAC CACCCGAACCAAGACAGGAGTGGGCTACCCGCAGCTGAGTGCGGTGATAGAGTGTGCTGACTCCGCCCAT GGCCTCAAGGGGCACATCATCTCTGATGGAGGCTGCACATGTCCAGGAGATGTCGCCAAAGCCTTTGGAG CTGGTGCAGACTTTGTCATGCTGGGAGGCATGTTTTCAGGCCACACCGAGTGTGCAGGTGAGGTGATCGA GAGGAACGGGCAGAAGCTGAAACTCTTCTATGGCATGAGCTCAGACACAGCCATGAAGAAACACGCAGGA GGCGTCGCCGAGTACAGGGCCTCCGAGGGCAAGACCGTGGAAGTGCCTTACAAAGGGGACGTGGAGAACA CCATTCTGGATATCCTTGGAGGACTGCGCTCCACCTGTACCTACGTCGGGGCTGCCAAGCTCAAAGAGCT CAGCAGGAGAGCCACGTTCATCCGGGTGACCCAACAGCACAACACGGTGTTTGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205188 representing BC010827 Red=Cloning site Green=Tags(s) MPRIDADLKLDFKDVLLRPKRSSLKSRSEVDLERTFTFRNSKQTYSGIPIIVANMDTVGTFEMAVVMSQH AMFTAVHKHYSLDDWKCFAETHPECLQHVAVSSGSGQNDLERMSRILEAVPQVKFICLDVANGYSEHFVE FVKLVRSKFPEHTIMAGNVVTGEMVEELILSGADIIKVGVGPGSVCTTRTKTGVGYPQLSAVIECADSAH GLKGHIISDGGCTCPGDVAKAFGAGADFVMLGGMFSGHTECAGEVIERNGQKLKLFYGMSSDTAMKKHAG GVAEYRASEGKTVEVPYKGDVENTILDILGGLRSTCTYVGAAKLKELSRRATFIRVTQQHNTVFG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC010827
ORF Size	1037 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010827, AAH10827
RefSeq Size	1595 bp
RefSeq ORF	1037 bp
Locus ID	66355
Gene Summary	A similar gene in human encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP. The protein also functions in the re-utilization of free intracellular bases and purine nucleosides. [provided by RefSeq, May 2015]

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