United States
Germany
Japan
United Kingdom
China
Prmt1 (BC002249) Mouse Tagged ORF Clone
CAT＃: MG205164
- TrueORF®
Prmt1 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 1 (cDNA clone MGC:7572 IMAGE:3493263)
ORF Plasmid: DDK
"BC002249" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,190.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6720434D09Rik; AW214366; Hrmt1l2; Mrmt1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205164 representing BC002249
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTTTCCTGTGGCCAAGCAGAAAGTAGTGAGAAGCCCAACGCTGAGGACATGACATCCAAAGACT ACTACTTTGACTCCTATGCCCACTTTGGCATCCACGAGGAGATGCTGAAGGATGAGGTGCGCACCCTCAC ATACCGCAACTCCATGTTTCACAATCGGCATCTCTTCAAAGACAAGGTGGTGCTGGACGTGGGCTCAGGC ACTGGCATCCTCTGCATGTTTGCTGCCAAGGCGGGGGCCCGCAAGGTTATTGGGATTGAGTGTTCCAGTA TCTCCGATTATGCTGTGAAGATTGTCAAAGCCAACAAGTTAGACCATGTGGTGACCATCATCAAGGGCAA GGTGGAGGAGGTGGAGCTGCCCGTGGAGAAGGTGGACATCATCATCAGCGAGTGGATGGGTTACTGCCTC TTCTACGAGTCCATGCTCAACACCGTGCTGCATGCTCGGGACAAGTGGCTGGCACCCGATGGCCTCATCT TCCCAGACCGGGCCACCTTGTATGTGACAGCCATTGAGGACCGACAATATAAAGACTACAAGATCCACTG GTGGGAGAACGTGTATGGCTTTGATATGTCCTGCATTAAAGACGTGGCCATCAAGGAGCCCCTGGTGGAC GTGGTGGACCCAAAGCAGCTGGTCACCAATGCCTGCCTCATAAAGGAGGTGGACATCTACACAGTCAAGG TGGAGGACCTGACCTTCACCTCCCCCTTCTGCCTGCAAGTGAAGAGGAACGACTACGTGCATGCGTTGGT GGCTTACTTCAACATCGAGTTCACCCGATGCCACAAGAGGACCGGCTTCTCCACCAGTCCTGAGTCCCCG TACACACACTGGAAGCAGACTGTGTTCTACATGGAGGACTACCTAACAGTGAAGACTGGCGAGGAGATCT TTGGCACCATTGGAATGAGGCCCAATGCCAAAAACAATCGTGACTTGGACTTTACCATCGACCTGGACTT CAAGGGTCAGCTGTGTGAGCTCTCTTGTTCCACCGACTACCGGATGCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205164 representing BC002249
Red=Cloning site Green=Tags(s) MEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSG TGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCL FYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVD VVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESP YTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC002249 |
| ORF Size | 1031 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC002249, AAH02249 |
| RefSeq Size | 1213 bp |
| RefSeq ORF | 1031 bp |
| Locus ID | 15469 |
| Gene Summary | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:15327772, PubMed:19858291). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation (By similarity). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (By similarity). May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway (By similarity). Methylates RBM15, promoting ubiquitination and degradation of RBM15 (By similarity). Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:19858291).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200097 | Prmt1 (untagged) - Mouse protein arginine N-methyltransferase 1 (cDNA clone MGC:7572 IMAGE:3493263), (10ug) |
CNY 3,656.00 |
|
| MR205164 | Prmt1 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 1 (cDNA clone MGC:7572 IMAGE:3493263) |
CNY 3,656.00 |
|
| MR205164L3 | Lenti ORF clone of Prmt1 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 1 (cDNA clone MGC:7572 IMAGE:3493263) |
CNY 3,900.00 |
|
| MR205164L4 | Lenti ORF clone of Prmt1 (mGFP-tagged) - Mouse protein arginine N-methyltransferase 1 (cDNA clone MGC:7572 IMAGE:3493263) |
CNY 3,900.00 |
