Gnb1 (BC013058) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA409223; C77571; Gnb-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205102 representing BC013058 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAACTTGACCAGCTGCGGCAGGAGGCCGAGCAACTGAAGAACCAAATTAGAGATGCTCGTAAAG CGTGTGCCGATGCGACTCTTTCTCAGATCACAAACAATATTGATCCAGTGGGAAGAATCCAAATGCGGAC CAGGAGAACACTGAGGGGGCATCTGGCAAAGATTTATGCCATGCACTGGGGCACAGACTCAAGGCTCCTT GTCAGCGCCTCTCAGGATGGAAAACTCATCATCTGGGACAGTTATACCACAAACAAGGTTCATGCCATCC CTCTGCGCTCCTCTTGGGTCATGACCTGCGCATACGCTCCTTCTGGGAATTATGTGGCCTGTGGTGGCCT GGATAACATCTGCTCCATTTACAACCTGAAAACTCGTGAAGGGAATGTGCGTGTGAGTCGTGAGCTGGCG GGACACACAGGTTATCTGTCCTGTTGCCGGTTCCTGGATGACAATCAGATAGTTACCAGTTCTGGAGACA CCACATGTGCCCTGTGGGACATCGAGACTGGCCAGCAGACAACCACATTTACTGGACACACTGGAGATGT CATGAGCCTGTCTCTTGCTCCTGACACCAGACTGTTTGTCTCTGGTGCTTGTGATGCTTCAGCCAAGCTC TGGGATGTCCGAGAAGGGATGTGCCGGCAGACCTTTACAGGACACGAGTCTGACATCAATGCCATATGTT TCTTTCCCAATGGCAATGCCTTTGCCACTGGCTCAGACGATGCCACATGCAGGCTGTTTGACCTCCGTGC AGACCAGGAGCTCATGACCTACTCCCATGACAACATTATCTGTGGTATCACATCTGTTTCCTTCTCCAAG AGTGGCCGCCTCCTCCTTGCTGGGTATGATGACTTCAACTGTAATGTCTGGGATGCACTCAAAGCTGACA GAGCAGGTGTCTTAGCTGGACACGACAACCGAGTCAGCTGCTTGGGGGTGACTGATGATGGCATGGCTGT GGCAACAGGGTCCTGGGACAGCTTCCTCAAGATCTGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205102 representing BC013058 Red=Cloning site Green=Tags(s) MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLL VSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELA GHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKL WDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSK SGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC013058
ORF Size	1022 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013058, AAH13058
RefSeq Size	1714 bp
RefSeq ORF	1022 bp
Locus ID	14688
Gene Summary	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (By similarity).[UniProtKB/Swiss-Prot Function]

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