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Catsper3 (BC089518) Mouse Tagged ORF Clone
CAT＃: MG205090
- TrueORF®
Catsper3 (tGFP-tagged) - Mouse cation channel, sperm associated 3 (cDNA clone MGC:107210 IMAGE:6774298)
ORF Plasmid: DDK
"BC089518" in other vectors (4)
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CNY 2,090.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4921522D01Rik; CACRC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205090 representing BC089518
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCACCACGGTCACCACCAACTCCTTTTTACTGGTCTTGGGGACTAATTATGACATACAATTCGAGT TTTTCAGAACCTTTGAGGTCTCAGAGCTTTTCTTTGTATCTGTCTATGTCTGCGAGTTCCTCATGAAGGT CTATGTGGACCCCATTACATACTGGAAGGATGGCTATAACATACTGGATGTGATCATTCTCATCATTCTC ACCATACCCTATCTCCTCCGCAAAATCAAGGGGAATCATTCTGCATACCTCCACTTTGCTGATGGCATCC AGTCTCTACGAATCCTCAAGCTTATCTCCTACAGTAGGGGCATCAGGACACTCATCATCGCTGTGGGGGA GACGGTCTACACTGTGGCCTCGGTGCTGACGCTGCTCTTCCTCCTCATGTTTGTGTTCGCGATCCTGGGA TTCTGCCTATTTGGCGTGACGGACAGAGGCGACCTGGAGAACTGGGGGAACCTGGCTTCAGCTTTCTTTA CTCTCTTCAGTTTGGCCACGGTTGATGGCTGGACTGACCTGCAGGAAGAGCTGGACAAGAGGAAGTTTAC TGTGAGCCGGGCGTTTACTATCCTCTTCATCTTGCTTGCATCCTTCATCTTCCTCAACATGTTTGTGGGT GTGATGATCATGCACACGGAGGATTCCATGAAAAAGTTTGAGCGGGATCTGACGTTGGAGAGGAACCTTG CGATTATGGAGGAGAAGCAAATAATCCTGAAACGCCAGCAAGAGGAGGTCAACAGGCTGATGAACACACA GAAAAGTGGTAGCATGAACTTCATTGATATGGTGGAGGGCTTCAAGAAGACCCTGCGGCACACAGACCCC ATGGTTCTGGATGACTTCAGCACTAGTCTCTCCTTCATTGATATCTACTTGGTCACTCTGGACAACCAAG ATGTTATTGTCAGCAAGCTTCAGGAGCTCTACTGTGAGATTGTGAACGTGCTGAGCCTGATGTTGGAAGA CATGCCCAAGGAGAGCTCGTCCAGCCTCTCGGGACTAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205090 representing BC089518
Red=Cloning site Green=Tags(s) MITTVTTNSFLLVLGTNYDIQFEFFRTFEVSELFFVSVYVCEFLMKVYVDPITYWKDGYNILDVIILIIL TIPYLLRKIKGNHSAYLHFADGIQSLRILKLISYSRGIRTLIIAVGETVYTVASVLTLLFLLMFVFAILG FCLFGVTDRGDLENWGNLASAFFTLFSLATVDGWTDLQEELDKRKFTVSRAFTILFILLASFIFLNMFVG VMIMHTEDSMKKFERDLTLERNLAIMEEKQIILKRQQEEVNRLMNTQKSGSMNFIDMVEGFKKTLRHTDP MVLDDFSTSLSFIDIYLVTLDNQDVIVSKLQELYCEIVNVLSLMLEDMPKESSSSLSGLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC089518 |
| ORF Size | 1022 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC089518, AAH89518 |
| RefSeq Size | 1511 bp |
| RefSeq ORF | 1022 bp |
| Locus ID | 76856 |
| Gene Summary | Voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Activated by intracellular alkalinization.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218490 | Catsper3 (untagged) - Mouse cation channel, sperm associated 3 (cDNA clone MGC:107210 IMAGE:6774298), (10ug) |
CNY 6,940.00 |
|
| MR205090 | Catsper3 (Myc-DDK-tagged) - Mouse cation channel, sperm associated 3 (cDNA clone MGC:107210 IMAGE:6774298) |
CNY 3,656.00 |
|
| MR205090L3 | Lenti ORF clone of Catsper3 (Myc-DDK-tagged) - Mouse cation channel, sperm associated 3 (cDNA clone MGC:107210 IMAGE:6774298) |
CNY 3,800.00 |
|
| MR205090L4 | Lenti ORF clone of Catsper3 (mGFP-tagged) - Mouse cation channel, sperm associated 3 (cDNA clone MGC:107210 IMAGE:6774298) |
CNY 3,800.00 |
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