Dnd1 (NM_173383) Mouse Tagged ORF Clone
CAT#: MG205085
- TrueORF®
Dnd1 (tGFP-tagged) - Mouse dead end homolog 1 (zebrafish) (Dnd1)
ORF Plasmid: DDK
"NM_173383" in other vectors (4)
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CNY 3,710.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BC034897; RBMS4; Ter |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205085 representing NM_173383
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTCCCTCCCATCCCCACCCCTACCCCCCCCCCCCAGCTTTATCCTGGAGCTTAAAAACATCCTTG TGGACCACAGTAACCAACAAAATCCTTTCCCTCCAGGCTGGGTGGGCAGCCCGCCACCGTCAGGGTCCGA GGTGTATATCGGACGACTTCCGCAGGACGTGTACGAGCACCAGCTAATCCCACTCTTCCAGCGCGTGGGG CGCCTCTATGAGTTCCGCCTAATGATGACCTTCAGTGGTTTGAACCGCGGCTTTGCCTATGCGCGCTACA GCTCGCGGCGCGGTGCCCAGGCCGCCATTGCTACGCTGCACAACCACCAGCTGCGGCCTTCTTGCCAGCT CCTGGTGTGCCGAAGCACCGAGAAGTGCGAGCTGACGGTGGACGGGCTGCCTCTTAGCTTAAACCGCCGA GCGTTACTGCTCGCCCTGCAACCGTTCGGTCCCTGCCTCCAGGAGACACTGCTGCTACCCAGCCCTGGGT CTGCGCCCTCTCAGATTGCGCTGCTCAAGTTCAGTACGCACCGAGCTGCTGCTATGGCCAAAAAGGCCCT GGTAGAAGGTCAGTCACGCCTCTGTGGAGAGCAGGTGGCTGTGGAGTGGCTCAAGCCAGACCTAAAGCAG CACTTCCGCCAGCAGCTTGCAGGTCCCTCACTGCGGTTCCTCAGGCCAGATGTCAGCCAACTAACCCAGA CCAGGGAGAAGCTGGGGTCCCAAGGGGCTCGGGCTGCCCTGCAACTGCTGTGTCAACGGATGAAGCTAGG CAGCCCAGTGTTCCTGACCAAGTGTTTAGGAACAGGGCCTGCTGGCTGGCATCGCTTTTGGTACCAGGTG GTGATCCCTGGGCATCCAGTGCCTTTCAGTGGCCTCATTTGGGTTGTGTTGGCCTCTGATTGGCAGGATG GGCATGAGGTGGCCAAGGATGCTGTGTCCGCACAGCTGCTGGAGGCACTGAGTGAACCTAGGACCAGCTT GTGGTCTCCTGGGGCTGAAGCAGGTACTATGGTTAAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205085 representing NM_173383
Red=Cloning site Green=Tags(s) MVSLPSPPLPPPPSFILELKNILVDHSNQQNPFPPGWVGSPPPSGSEVYIGRLPQDVYEHQLIPLFQRVG RLYEFRLMMTFSGLNRGFAYARYSSRRGAQAAIATLHNHQLRPSCQLLVCRSTEKCELTVDGLPLSLNRR ALLLALQPFGPCLQETLLLPSPGSAPSQIALLKFSTHRAAAMAKKALVEGQSRLCGEQVAVEWLKPDLKQ HFRQQLAGPSLRFLRPDVSQLTQTREKLGSQGARAALQLLCQRMKLGSPVFLTKCLGTGPAGWHRFWYQV VIPGHPVPFSGLIWVVLASDWQDGHEVAKDAVSAQLLEALSEPRTSLWSPGAEAGTMVKQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_173383 |
ORF Size | 1020 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_173383.1, NP_775559.1 |
RefSeq Size | 1825 bp |
RefSeq ORF | 1059 bp |
Locus ID | 213236 |
UniProt ID | Q6VY05 |
Gene Summary | RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression (By similarity). Relieves miRNA repression in germline cells (By similarity). Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs (By similarity). Sequence-specific RNA-binding factor that binds specifically to U-rich regions (URRs) in the 3' untranslated region (3' UTR) of several mRNAs (By similarity). Does not bind to miRNAs (By similarity). Isoform 1 may play a role during primordial germ cell (PGC) survival. However, does not seem to be essential for PGC migration.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201408 | Dnd1 (untagged) - Mouse dead end homolog 1 (zebrafish) (Dnd1), (10ug) |
CNY 3,656.00 |
|
MR205085 | Dnd1 (Myc-DDK-tagged) - Mouse dead end homolog 1 (zebrafish) (Dnd1) |
CNY 3,656.00 |
|
MR205085L3 | Lenti ORF clone of Dnd1 (Myc-DDK-tagged) - Mouse dead end homolog 1 (zebrafish) (Dnd1) |
CNY 5,230.00 |
|
MR205085L4 | Lenti ORF clone of Dnd1 (mGFP-tagged) - Mouse dead end homolog 1 (zebrafish) (Dnd1) |
CNY 5,230.00 |