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Prr14 (BC006909) Mouse Tagged ORF Clone
CAT＃: MG205081
- TrueORF®
Prr14 (tGFP-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775)
ORF Plasmid: DDK
"BC006909" in other vectors (4)
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CNY 2,090.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC6888 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205081 representing BC006909
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGATTATAATTATGCAAACAACAAAAGTCCGATCAAAGCTTGAAAGCTTTGCTGATATCTTCTTCA CACCTAACAAAACACCACAGCCTCCACCCCCTTCACCTCCCATGAAATTAGAATTGAAGATTGCCATCTC GGAGGCTGAGCAGTCCAGGGCTACTGAGAAAATTACATCTGTCAGCCCCAGGCCCCCTATCCGCCAGTGG CGGACTCAGTGCAATTCCCTAGCACCTGTCTCTAAGTCATCTCTGGGACGAAGCTACTCCTGCCCTGATC TAGGGCCCCCTGACCCAGGTAGCTGGCCTCCTGTCCCATCCCAGCCAAGCCAGTCCAGGCCCCGGAGACA TACTGTGGGTTGTGGGGAGATGGCCCGGACCCCACCACCTCCTCGACCCTGTCTCCGGAAAGAGGTCTTC CCTCTTGGAGGAGTGGGAGTCTCTCCTTCTCTCACTACATCTTGCTCAGCCAATGCCCCTGCTTCCTTCT TCTGCGAGCCGGCAGAACCCAGGTTGGGTTCAACGAAGGGGAAGGAGTTAAGAGCCTCAAAAGACAAGGT GTTTTCTGATCCAGAGACCAAGACCATGGGGAAGGTTTCTAGATTCAGAATACGGAGGACACCTGTCCGT CTCCAACCAAACCTTACACCAATGGGGCTGCCTCGACCAATCAGGTTGAATAAGAAGGAGTTCACTTTGG AAGAAATTTATACAAATAAGAACTACCAATCACCCACAACCAGAAGGACCTTTGAGACCATCTTTGAGGA ACCACGGGAGCGCAATGGAACTCTGATTTTTACCAGCTCTAGGAAGCTCCGAAGGGCAGTAGAATTTCGG GACAGTAGCCTTCCTCGATCCCGGCGACCATCACGAGGGGTCCGCACCGCAGCAAGCAGGACCCTTACTC CCAACCTGGCACCCAGCCAGGATGTGGGGTCTCTACTGCAAGAAAGGCTGAGGGAGCTAGATGCCTTGCT CCTGGAGGAGGAGACAGATAAGGAACACCCTTGTCACCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205081 representing BC006909
Red=Cloning site Green=Tags(s) MSIIIMQTTKVRSKLESFADIFFTPNKTPQPPPPSPPMKLELKIAISEAEQSRATEKITSVSPRPPIRQW RTQCNSLAPVSKSSLGRSYSCPDLGPPDPGSWPPVPSQPSQSRPRRHTVGCGEMARTPPPPRPCLRKEVF PLGGVGVSPSLTTSCSANAPASFFCEPAEPRLGSTKGKELRASKDKVFSDPETKTMGKVSRFRIRRTPVR LQPNLTPMGLPRPIRLNKKEFTLEEIYTNKNYQSPTTRRTFETIFEEPRERNGTLIFTSSRKLRRAVEFR DSSLPRSRRPSRGVRTAASRTLTPNLAPSQDVGSLLQERLRELDALLLEEETDKEHPCHL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC006909 |
| ORF Size | 1022 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006909 |
| RefSeq Size | 1268 bp |
| RefSeq ORF | 1022 bp |
| Locus ID | 233895 |
| Gene Summary | Functions in tethering peripheral heterochromatin to the nuclear lamina during interphase, possibly through the interaction with heterochromatin protein CBX5/HP1 alpha (By similarity). Might play a role in reattaching heterochromatin to the nuclear lamina at mitotic exit (By similarity). Promotes myoblast differentiation during skeletal myogenesis, possibly by stimulating transcription factor MyoD activity via binding to CBX5/HP1 alpha (PubMed:25906157) (By similarity). Involved in the positive regulation of the PI3K-Akt-mTOR signaling pathway and in promoting cell proliferation, possibly via binding to GRB2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200258 | Prr14 (untagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775), (10ug) |
CNY 3,656.00 |
|
| MR205081 | Prr14 (Myc-DDK-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) |
CNY 3,656.00 |
|
| MR205081L3 | Lenti ORF clone of Prr14 (Myc-DDK-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) |
CNY 3,800.00 |
|
| MR205081L4 | Lenti ORF clone of Prr14 (mGFP-tagged) - Mouse proline rich 14 (cDNA clone MGC:6888 IMAGE:2651775) |
CNY 3,800.00 |
