Txnl2 (BC087885) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	PICOT; Txnl2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205019 representing BC087885 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGGCGGCCGAGGCAGGCGAGGCAGCCGTGGCAGTGGTGGAAGTCGGCTCTGCCCAGCAGT TTGAAGAGCTACTGCGCCTCAAAACCAAGTCACTCCTTGTGGTTCATTTCTGGGCACCATGGGCTCCACA GTGTGTACAGATGAACGATGTCATGGCAGAGTTAGCTAAAGAACACCCTCATGTTTCATTTGTGAAGCTG GAAGCCGAAGCTGTTCCTGAAGTATCTGAAAAATATGAAATTAGCTCTGTCCCCACCTTCCTGTTCTTCA AGAATTCTCAGAAAGTCGACCGGTTAGATGGTGCACATGCCCCAGAGTTGACCAAAAAAGTCCAGCGACA CGTGTCTAGCGGAGCCTTTCCTCCTAGTACTAATGAACATCTTAAAGAAGACCTCAGCCTTCGCCTGAAA AAGCTGACTCACGCTGCCCCCTGCATGCTGTTCATGAAGGGAACACCTCAAGAACCACGCTGTGGTTTCA GCAAGCAGATGGTGGAAATCCTTCACAAACACAATATTCAGTTCAGCAGCTTTGATATCTTCTCAGATGA AGAAGTTCGACAGGGGCTCAAAACGTACTCTAATTGGCCCACCTATCCTCAGCTCTATGTTTCTGGAGAG CTAATAGGAGGACTTGACATAATTAAGGAGCTGGAAGCATCAGAAGAGCTGGACACGATCTGTCCCAAAG CACCCAAGTTAGAGGAAAGGCTCAAAGTGCTGACCAATAAAGCTTCCGTGATGCTCTTTATGAAAGGAAA CAAACAGGAAGCAAAATGTGGATTCAGCAAACAAATTCTGGAAATACTGAATAGTACTGGGGTTGAATAT GAAACTTTTGATATACTGGAAGATGAAGAAGTGCGTCAGGGATTAAAAACGTTCTCAAATTGGCCAACCT ACCCTCAGCTGTATGTGAGAGGGGATCTTGTTGGAGGATTGGATATTGTCAAGGAACTGAAAGACAACGG TGAATTGCTGCCTATACTGAAAGGAGAAAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205019 representing BC087885 Red=Cloning site Green=Tags(s) MAAGAAEAGEAAVAVVEVGSAQQFEELLRLKTKSLLVVHFWAPWAPQCVQMNDVMAELAKEHPHVSFVKL EAEAVPEVSEKYEISSVPTFLFFKNSQKVDRLDGAHAPELTKKVQRHVSSGAFPPSTNEHLKEDLSLRLK KLTHAAPCMLFMKGTPQEPRCGFSKQMVEILHKHNIQFSSFDIFSDEEVRQGLKTYSNWPTYPQLYVSGE LIGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVEY ETFDILEDEEVRQGLKTFSNWPTYPQLYVRGDLVGGLDIVKELKDNGELLPILKGEN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC087885
ORF Size	1013 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC087885, AAH87885
RefSeq Size	1257 bp
RefSeq ORF	1013 bp
Locus ID	30926
Gene Summary	Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (By similarity). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (PubMed:16809552, PubMed:18258855, PubMed:18929570). Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity (By similarity).[UniProtKB/Swiss-Prot Function]

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