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Txnl2 (BC033506) Mouse Tagged ORF Clone
CAT＃: MG205008
- TrueORF®
Txnl2 (tGFP-tagged) - Mouse thioredoxin-like 2 (cDNA clone MGC:35818 IMAGE:2651132)
ORF Plasmid: DDK
"BC033506" in other vectors (3)
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CNY 3,710.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PICOT; Txnl2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205008 representing BC033506
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGGCGGCCGAGGCAGGCGAGGCAGCCGTGGCAGTGGTGGAAGTCGGCTCTGCCCAGCAGT TTGAAGAGCTACTGCGCCTCAAAACCAAGTCACTCCTTGTGGTTCATTTCTGGGCACCATGGGCTCCACA GTGTGTACAGATGAACGATGTCATGGCAGAGTTAGCTAAAGAACACCCTCATGTTTCATTTGTGAAGCTG GAAGCCGAAGCTGTTCCTGAAGTATCTGAAAAATATGAAATTAGCTCTGTCCCCACCTTCCTGTTCTTCA AGAATTCTCAGAAAGTCGACCGGTTAGATGGTGCACATGCCCCAGAGTTGACCAAAAAAGTCCAGCGACA CGTGTCTAGCGGAGCCTTTCCTCCTAGTACTAATGAACATCTTAAAGAAGACCTCAGCCTTCGCCTGAAA AAGCTGACTCACGCTGCCCCCTGCATGCTGTTCATGAAGGGAACACCTCAAGAACCGCGCTGTGGTTTCA GCAAGCAGATGGTGGAAATCCTTCACAAACACAATATTCAGTTCAGCAGCTTTGATATCTTCTCAGATGA AGAAGTTCGACAGGGGCTCAAAACGTACTCTAATTGGCCCACCTATCCTCAGCTCTATGTTTCTGGAGAG CTAATAGGAGGACTTGACATAATTAAGGAGCTGGAAGCATCAGAAGAGCTGGACACGATCTGTCCCAAAG CACCCAAGTTAGAGGAAAGGCTCAAAGTGCTGACCAATAAAGCTTCCGTGATGCTCTTTATGAAAGGAAA CAAACAGGAAGCAAAATGTGGATTCAGCAAACAAATTCTGGAAATACTGAATAGTACTGGGGTTGAATAT GAAACTTTTGATATACTGGAAGATGAAGAAGTGCGTCAGGGATTAAAAACGTTCTCAAATTGGCCAACCT ACCCTCAGCTGTATGTGAGAGGGGATCTTGTTGGAGGATTGGATATTGTCAAGGAACTGAAAGACAACGG TGAATTGCTGCCTATACTGAAAGGAGAAAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205008 representing BC033506
Red=Cloning site Green=Tags(s) MAAGAAEAGEAAVAVVEVGSAQQFEELLRLKTKSLLVVHFWAPWAPQCVQMNDVMAELAKEHPHVSFVKL EAEAVPEVSEKYEISSVPTFLFFKNSQKVDRLDGAHAPELTKKVQRHVSSGAFPPSTNEHLKEDLSLRLK KLTHAAPCMLFMKGTPQEPRCGFSKQMVEILHKHNIQFSSFDIFSDEEVRQGLKTYSNWPTYPQLYVSGE LIGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVEY ETFDILEDEEVRQGLKTFSNWPTYPQLYVRGDLVGGLDIVKELKDNGELLPILKGEN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC033506 |
| ORF Size | 1013 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC033506, AAH33506 |
| RefSeq Size | 1260 bp |
| RefSeq ORF | 1013 bp |
| Locus ID | 30926 |
| Gene Summary | Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (By similarity). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (PubMed:16809552, PubMed:18258855, PubMed:18929570). Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR205008 | Txnl2 (Myc-DDK-tagged) - Mouse thioredoxin-like 2 (cDNA clone MGC:35818 IMAGE:2651132) |
CNY 3,330.00 |
|
| MR205008L3 | Lenti ORF clone of Txnl2 (Myc-DDK-tagged) - Mouse thioredoxin-like 2 (cDNA clone MGC:35818 IMAGE:2651132) |
CNY 5,230.00 |
|
| MR205008L4 | Lenti ORF clone of Txnl2 (mGFP-tagged) - Mouse thioredoxin-like 2 (cDNA clone MGC:35818 IMAGE:2651132) |
CNY 5,230.00 |
