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Dhdds (NM_026144) Mouse Tagged ORF Clone
CAT＃: MG204923
- TrueORF®
Dhdds (tGFP-tagged) - Mouse dehydrodolichyl diphosphate synthase (Dhdds)
ORF Plasmid: DDK
"NM_026144" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3222401G21Rik; CIT; DS; HDS; W91638 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204923 representing NM_026144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATGGATCAAAGAAGGAGAGCTGTCACTGTGGGAACGGTTCTGTGCTAACATCATAAAGGCTGGCC CAGTACCCAAACATATCGCGTTCATAATGGACGGCAACCGTCGCTATGCCAAGAAGTGTCAGGTGGAGCG CCAGGAGGGCCACACACAGGGCTTCAATAAGCTTGCTGAGACTCTCCGCTGGTGTTTGAACCTGGGCATC CTAGAAGTGACTGTCTACGCATTCAGCATTGAGAACTTCAAACGTTCCAAGAGTGAGGTTGACGGACTCC TGGATCTAGCCAGACAGAAGTTCAGCTGCTTGATGGAAGAACAGGAGAAGTTGCAGAAGCACGGGGTGTG CATCCGCGTCCTGGGTGATCTGCATCTGCTGCCCTTGGACCTCCAGGAGAAGATTGCGCATGCCATCCAG GCTACTAAGAACTACAATAAGTGTTTCCTCAATGTCTGCTTTGCATACACATCACGTCATGAGATTGCCA ATGCTGTGAGAGAGATGGCCTGGGGCGTGGAACAAGGTCTGCTGGAACCCAGTGATGTCTCCGAGTCTCT GCTCGATAAGTGCCTCTATAGCAACCACTCTCCTCATCCCGACATCCTGATCCGGACTTCTGGGGAGGTG CGGCTGAGTGACTTCTTGCTCTGGCAGACGTCCCATTCCTGCCTCGTGTTCCAGCCTGTCCTGTGGCCAG AATACACATTTTGGAACCTGTGTGAGGCAATTCTGCAGTTTCAGAGGAACCATGGTGCACTTCAGAAGGC CCGAGACATGTACGCTGAGGAGCGGAAGAGGCGCCAGCTGGAGAGGGACCAGGCCGCAGTGACAGAGCAG CTGCTTCGAGAGGGGCTCCAGGCCAGTGGGGATGCCCAACTCCGACGGACACGCTTGCACAAACTCTCCA CCAAACGGGAAGAGCGAGTCCAAGGCTTCCTGAAGGCCTTGGAACTTAAACGGGCCAACTGGCTGGCACT TTGGGGCACTGCATCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204923 representing NM_026144
Red=Cloning site Green=Tags(s) MSWIKEGELSLWERFCANIIKAGPVPKHIAFIMDGNRRYAKKCQVERQEGHTQGFNKLAETLRWCLNLGI LEVTVYAFSIENFKRSKSEVDGLLDLARQKFSCLMEEQEKLQKHGVCIRVLGDLHLLPLDLQEKIAHAIQ ATKNYNKCFLNVCFAYTSRHEIANAVREMAWGVEQGLLEPSDVSESLLDKCLYSNHSPHPDILIRTSGEV RLSDFLLWQTSHSCLVFQPVLWPEYTFWNLCEAILQFQRNHGALQKARDMYAEERKRRQLERDQAAVTEQ LLREGLQASGDAQLRRTRLHKLSTKREERVQGFLKALELKRANWLALWGTASA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026144 |
| ORF Size | 999 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026144.2, NP_080420.2 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 1002 bp |
| Locus ID | 67422 |
| UniProt ID | Q99KU1 |
| Gene Summary | With NUS1, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208069 | Dhdds (untagged) - Mouse dehydrodolichyl diphosphate synthase (Dhdds), (10ug) |
CNY 3,990.00 |
|
| MR204923 | Dhdds (Myc-DDK-tagged) - Mouse dehydrodolichyl diphosphate synthase (Dhdds) |
CNY 3,656.00 |
|
| MR204923L3 | Lenti ORF clone of Dhdds (Myc-DDK-tagged) - Mouse dehydrodolichyl diphosphate synthase (Dhdds) |
CNY 4,750.00 |
|
| MR204923L4 | Lenti ORF clone of Dhdds (mGFP-tagged) - Mouse dehydrodolichyl diphosphate synthase (Dhdds) |
CNY 4,750.00 |
