Rfng (BC066023) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204901 representing BC066023 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGTGCGCGGCGGGTGTTGTGCCGGGCCTGCCTCGCGCTGGCCGCGGTCCTGGCTGTGTTGCTGC TACTGCCGCTGCCGCTACCGCTGCCGCTGCCTCGCGCGCCCGCACCGGACCCCGATCGGGTCCCGACCCG GAGCCTGACCCTCGAGGGAGACCGCCTGCAACCCGACGACGTCTTCATTGCAGTCAAGACCACTCGGAAG AACCACGGCCCGCGCCTGCGGCTGCTGCTGCGTACCTGGATCTCACGAGCCCCACGGCAGACGTTCATTT TCACCGATGGAGACGACCCTGAGCTCCAGATGCTGGCAGGCGGCCGCATGATCAACACCAATTGCTCTGC TGTGCGCACCCGCCAAGCACTGTGCTGCAAAATGTCGGTGGAATATGATAAGTTCCTAGAATCTGGACGA AAATGGTTCTGCCACGTGGATGATGACAACTACGTGAACCCCAAAAGCCTGCTGCACCTGCTTTCCACCT TCTCTTCCAACCAGGACATCTACCTGGGGCGACCTAGCCTGGACCACCCCATCGAAGCCACAGAGAGGGT CCAAGGCGGTGGCACCTCAAACACAGTGAAATTCTGGTTTGCTACTGGTGGGGCTGGGTTCTGCCTGAGC AGGGGCCTTGCCCTCAAAATGAGCCCGTGGGCCAGCCTTGGCAGTTTCATGAGCACAGCAGAGCGGGTTC GGCTGCCTGATGACTGCACTGTGGGATACATCGTGGAAGGACTTCTGGGCGCCCGTCTGCTCCATAGCCC CCTGTTCCACTCGCACCTGGAAAACCTGCAGAGGCTGCCGTCTGGTGCTATTTTGCAGCAGGTTACCTTG AGCTATGGGGGTCCTGAGAACCCACATAATGTGGTGAATGTAGCTGGCAGTTTCAACATACAGCAGGACC CTACACGGTTTCAGTCTGTGCACTGCCTTCTCTACCCAGACACCCACTGGTGTCCTATGAAGAACAGGGT TGAGGGAGCTTTCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204901 representing BC066023 Red=Cloning site Green=Tags(s) MSRARRVLCRACLALAAVLAVLLLLPLPLPLPLPRAPAPDPDRVPTRSLTLEGDRLQPDDVFIAVKTTRK NHGPRLRLLLRTWISRAPRQTFIFTDGDDPELQMLAGGRMINTNCSAVRTRQALCCKMSVEYDKFLESGR KWFCHVDDDNYVNPKSLLHLLSTFSSNQDIYLGRPSLDHPIEATERVQGGGTSNTVKFWFATGGAGFCLS RGLALKMSPWASLGSFMSTAERVRLPDDCTVGYIVEGLLGARLLHSPLFHSHLENLQRLPSGAILQQVTL SYGGPENPHNVVNVAGSFNIQQDPTRFQSVHCLLYPDTHWCPMKNRVEGAFQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066023
ORF Size	998 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066023, AAH66023
RefSeq Size	2141 bp
RefSeq ORF	998 bp
Locus ID	19719
Gene Summary	Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1 (PubMed:28089369). May be involved in limb formation and in neurogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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