Rfng (BC066023) Mouse Tagged ORF Clone

CAT#: MG204901

  • TrueORF®

Rfng (tGFP-tagged) - Mouse RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (cDNA clone MGC:90122



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204901 representing BC066023
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGTGCGCGGCGGGTGTTGTGCCGGGCCTGCCTCGCGCTGGCCGCGGTCCTGGCTGTGTTGCTGC
TACTGCCGCTGCCGCTACCGCTGCCGCTGCCTCGCGCGCCCGCACCGGACCCCGATCGGGTCCCGACCCG
GAGCCTGACCCTCGAGGGAGACCGCCTGCAACCCGACGACGTCTTCATTGCAGTCAAGACCACTCGGAAG
AACCACGGCCCGCGCCTGCGGCTGCTGCTGCGTACCTGGATCTCACGAGCCCCACGGCAGACGTTCATTT
TCACCGATGGAGACGACCCTGAGCTCCAGATGCTGGCAGGCGGCCGCATGATCAACACCAATTGCTCTGC
TGTGCGCACCCGCCAAGCACTGTGCTGCAAAATGTCGGTGGAATATGATAAGTTCCTAGAATCTGGACGA
AAATGGTTCTGCCACGTGGATGATGACAACTACGTGAACCCCAAAAGCCTGCTGCACCTGCTTTCCACCT
TCTCTTCCAACCAGGACATCTACCTGGGGCGACCTAGCCTGGACCACCCCATCGAAGCCACAGAGAGGGT
CCAAGGCGGTGGCACCTCAAACACAGTGAAATTCTGGTTTGCTACTGGTGGGGCTGGGTTCTGCCTGAGC
AGGGGCCTTGCCCTCAAAATGAGCCCGTGGGCCAGCCTTGGCAGTTTCATGAGCACAGCAGAGCGGGTTC
GGCTGCCTGATGACTGCACTGTGGGATACATCGTGGAAGGACTTCTGGGCGCCCGTCTGCTCCATAGCCC
CCTGTTCCACTCGCACCTGGAAAACCTGCAGAGGCTGCCGTCTGGTGCTATTTTGCAGCAGGTTACCTTG
AGCTATGGGGGTCCTGAGAACCCACATAATGTGGTGAATGTAGCTGGCAGTTTCAACATACAGCAGGACC
CTACACGGTTTCAGTCTGTGCACTGCCTTCTCTACCCAGACACCCACTGGTGTCCTATGAAGAACAGGGT
TGAGGGAGCTTTCCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204901 representing BC066023
Red=Cloning site Green=Tags(s)

MSRARRVLCRACLALAAVLAVLLLLPLPLPLPLPRAPAPDPDRVPTRSLTLEGDRLQPDDVFIAVKTTRK
NHGPRLRLLLRTWISRAPRQTFIFTDGDDPELQMLAGGRMINTNCSAVRTRQALCCKMSVEYDKFLESGR
KWFCHVDDDNYVNPKSLLHLLSTFSSNQDIYLGRPSLDHPIEATERVQGGGTSNTVKFWFATGGAGFCLS
RGLALKMSPWASLGSFMSTAERVRLPDDCTVGYIVEGLLGARLLHSPLFHSHLENLQRLPSGAILQQVTL
SYGGPENPHNVVNVAGSFNIQQDPTRFQSVHCLLYPDTHWCPMKNRVEGAFQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC066023
ORF Size 998 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066023, AAH66023
RefSeq Size 2141 bp
RefSeq ORF 998 bp
Locus ID 19719
Gene Summary Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in enhancement of NOTCH1 activation by DLL1 and JAG1 (PubMed:28089369). May be involved in limb formation and in neurogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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