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Spon2 (NM_133903) Mouse Tagged ORF Clone
CAT＃: MG204867
- TrueORF®
Spon2 (tGFP-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2)
ORF Plasmid: DDK
"NM_133903" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310045I24Rik; AI504350; M-spondin; Mindin; Mspondin |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204867 representing NM_133903
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACTTGAGTCTTGCCCTGGGCAGAGCTCTTTGGGTCTTCCTCCTGGCCATGATAGGCTCCACCA CGAGCCAGCCGCTGGGGGGAGAATCGGTTTGTACAGCCCGGCCTCTGGCTAGATACAGCATCACTTTCAT TGGCAAGTGGAGCCAGACAGCATTTCCCAAGCAGTACCCCCTGTTCCGGCCCCCTGCACAGTGGTCCTCT CTGCTGGGGGCAGCTCACAGCTCTGACTACAGCATGTGGCGGAAGAATGAGTATGTCAGCAATGGGCTGA GGGACTTTGCTGAGCGTGGTGAGGCCTGGGCACTGATGAAGGAGATCGAAGCTGCAGGAGAGAAACTTCA GAGTGTGCACGCAGTGTTCTCAGCTCCTGCCATCCCTAGCGGTACCGGGCAGACTTCCACAGAGTTAGAG GTGCACCCTAGGCACTCGCTGGTGTCCTTCGTGGTACGCATTGTCCCCAGCCCTGACTGGTTTGTGGGCA TCGACAGTCTGGACCTGTGTGAGGGAGGCCGCTGGAAGGAGCAGGTGGTCCTGGATCTTTATCCACACGA TGCAGGGACCGACAGTGGTTTCACCTTCTCCTCCCCTAACTTTGCAACTATCCCACAAGACACAGTGACT GAGATAACAGCCTCATCGCCCAGCCACCCAGCAAATTCATTCTACTACCCACGCCTCAAGTCCTTACCTC CCATCGCCAAAGTGACCTTCGTGCGGCTACAGCATAGTCCCAGGGCCTTTGCCCCACCTTCCCTGGACCT GGCCAGCCGAGGCAACGAAATCGTTGACAGCCTTTCAGTTCCAGAGACACCGCTGGACTGTGAGGTTTCC CTGTGGTCATCCTGGGGACTGTGTGGAGGACCATGTGGAAAGTTGGGAGCCAAGAGCAGAACTCGCTATG TCCGTGTTCAGCCTGCTAACAATGGGACTCCCTGTCCCGAGCTTGAAGAAGAGGCCGAGTGTGCCCCAGA TAACTGCGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204867 representing NM_133903
Red=Cloning site Green=Tags(s) MENLSLALGRALWVFLLAMIGSTTSQPLGGESVCTARPLARYSITFIGKWSQTAFPKQYPLFRPPAQWSS LLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAIPSGTGQTSTELE VHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGGRWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVT EITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQHSPRAFAPPSLDLASRGNEIVDSLSVPETPLDCEVS LWSSWGLCGGPCGKLGAKSRTRYVRVQPANNGTPCPELEEEAECAPDNCV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133903 |
| ORF Size | 990 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133903.1 |
| RefSeq Size | 2182 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 100689 |
| UniProt ID | Q8BMS2 |
| Gene Summary | Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Binds bacterial lipopolysaccharide. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201295 | Spon2 (untagged) - Mouse spondin 2, extracellular matrix protein (Spon2), (10ug) |
CNY 2,400.00 |
|
| MR204867 | Spon2 (Myc-DDK-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2) |
CNY 2,400.00 |
|
| MR204867L3 | Lenti ORF clone of Spon2 (Myc-DDK-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2) |
CNY 4,750.00 |
|
| MR204867L4 | Lenti ORF clone of Spon2 (mGFP-tagged) - Mouse spondin 2, extracellular matrix protein (Spon2) |
CNY 4,750.00 |
