Fbxl12 (BC054471) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	3110048D16Rik; Fbl12
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204773 representing BC054471 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTCTGTTTGACCTCCCAGACTTGGTGCTCTTGGAGATCTTCTCTTACCTCCCTGTCCGGGACC GGATCCGCATCTCCAGGGTCTGTCACCGCTGGAAGAGGCTAGTGGATGACCGGTGGCTATGGCGACACGT CGACCTGACGCTCTACACGATGCGGCCGAAAGTCATGTGGCACCTCCTGCGCCGGTACATGGCGTCCCGG CTCTACTCGTTGCGCATGGGCGGCTACCTGTTTTCTGGCTCTCAGGCCCCACAGCTGTCCCCCGCCTTGA TGAGGGCCCTGGGTCAGAAATGCCCCAATCTGAAGCGCCTGTGCCTGCACGTGGCTGACCTGAGCATGGT GCCTATCACCAGCCTTCCTAGCACACTGAGGACCCTGGAGCTGCACAGCTGCGAAATCTCCATGATCTGG TTGCAGAAAGAGCAGGACCCCACGGTGCTGCCTCTTCTGGAATGCATCGTGCTGGACCGAGTGCCGGCCT TCCGCGATGAGCATCTGCAGGGCCTTACCCGATTCCGAGCCCTGCGCTCGCTGGTGCTGGGCGGCACCTA CCGGGTCACTGAGACCGGGCTAGATGCCAGCCTGCAGGAGCTCAGCTACCTGCAAAGGCTTGAGGTGCTG GGCTGCACCCTGTCAGCTGACAGCACGCTGCTGGCCATCAGCCGCCACCTTCGAGATGTGCGCAAGATTC GGCTGACCGTTGGGGGCCTCTCAGCCCAGGGCCTGGTCTTCCTGGAGGGAATGCCTGTCCTGGAGAGTTT GTGCTTCCAGGGTCCCCTTATCACCCCAGACATGCCCACACCCACTCAGATTGTGTCCTCCTGCCTCACC ATGCCGAAGCTCAGAGTGCTTGAGGTGCAAGGGCTGGGCTGGGAGGGTCAGGAAGCAGAGAAAATCCTGT GCAAGGGCCTGCCCCACTGCATAGTTATTGTTAGGGCTTGTCCCAAAGAATCCATGGATTGGTGGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204773 representing BC054471 Red=Cloning site Green=Tags(s) MATLFDLPDLVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHVDLTLYTMRPKVMWHLLRRYMASR LYSLRMGGYLFSGSQAPQLSPALMRALGQKCPNLKRLCLHVADLSMVPITSLPSTLRTLELHSCEISMIW LQKEQDPTVLPLLECIVLDRVPAFRDEHLQGLTRFRALRSLVLGGTYRVTETGLDASLQELSYLQRLEVL GCTLSADSTLLAISRHLRDVRKIRLTVGGLSAQGLVFLEGMPVLESLCFQGPLITPDMPTPTQIVSSCLT MPKLRVLEVQGLGWEGQEAEKILCKGLPHCIVIVRACPKESMDWWM TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054471
ORF Size	980 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054471, AAH54471
RefSeq Size	2112 bp
RefSeq ORF	980 bp
Locus ID	30843
Gene Summary	Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia (By similarity).[UniProtKB/Swiss-Prot Function]

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