Ccnh (BC038861) Mouse Tagged ORF Clone

CAT#: MG204705

  • TrueORF®

Ccnh (tGFP-tagged) - Mouse cyclin H (cDNA clone MGC:47913 IMAGE:1446473)



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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


CCNH Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 6330408H09Rik; AI661354; AV102684; AW538719
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204705 representing BC038861
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATCACAGCAGCAGCCAGAAACGGCACTGGACCTTCGCTAGCGAGGAGCAGCTGGCGCGCCTGCGGG
CGGACGCCAACCGCAAATTCAAGTGCAAAGCTGTGGCTAACGGGAAGGTTCTTCCAAATGATCCAGTGTT
TCTGGAGCCTCATGAAGAATTGACACTTTGCAAGTACTATGAAAAAAGATTATTGGAATTCTGTTCAGTG
TTTAAACCAGCTATGCCACGGTCTGTTGTGGGTACAGCTTGTATGTATTTCAAGCGTTTTTATCTTAATA
ACTCAGTAATGGAATATCACCCTCGGATAATAATGCTTACTTGTGCATTTTTGGCCTGCAAAGTAGATGA
ATTCAATGTGTCTAGTCCCCAGTTTGTTGGAAATCTTCGAGAGAGTCCTCTTGGACAGGAGAGGGCACTG
GAACAGATTTTGGAATATGAACTACTACTTATACAACAACTTAATTTTCACCTTATTGTCCACAATCCAT
ACAGACCATTTGAAGGCTTCCTCATCGATATAAAGACTCGGTACCCCATGTTGGAGAATCCGGAGATTCT
GAGGAAAACAGCTGATGATTTTCTTAGTAGAATTGCATTGACAGATGCTTATCTTTTATACACACCTTCA
CAGATTGCCCTGACCGCCATTTTATCAAGTGCCTCTAGAGCTGGAATTACTATGGAAAGCTACTTATCAG
AGAGTCTAATGCTAAAAGAAAACAGAACTTGCCTGTCACAGTTACTGGATATAATGAAAAGCATGAGAAA
TCTAGTAAAAAAGTACGAGCCGCCCAGGTCTGACGAGGTTGCTGTCCTGAAACAGAAGCTGGAGCGGTGT
CATTCTTCTGACCTTGCACTTAATGCGGTTACGAAGAAGAGGAAAGGCTATGAAGATGATGACTATGTGT
CAAAGAAACCCAAACAGGAAGAGGAGGAATGGACTGATGACGACCTGGTAGATTCTCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204705 representing BC038861
Red=Cloning site Green=Tags(s)

MYHSSSQKRHWTFASEEQLARLRADANRKFKCKAVANGKVLPNDPVFLEPHEELTLCKYYEKRLLEFCSV
FKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQERAL
EQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDIKTRYPMLENPEILRKTADDFLSRIALTDAYLLYTPS
QIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSDEVAVLKQKLERC
HSSDLALNAVTKKRKGYEDDDYVSKKPKQEEEEWTDDDLVDSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC038861
ORF Size 971 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC038861, AAH38861
RefSeq Size 1186 bp
RefSeq ORF 971 bp
Locus ID 66671
Gene Summary Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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