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Peli2 (BC027062) Mouse Tagged ORF Clone
CAT＃: MG204612
- TrueORF®
Peli2 (tGFP-tagged) - Mouse pellino 2 (cDNA clone MGC:38603 IMAGE:5355240)
ORF Plasmid: DDK
"BC027062" in other vectors (4)
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CNY 3,420.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW047589; BB129927 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204612 representing BC027062
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCAGGTGGGCAGGTCAACAGAAAGCCCCATTGACTTCGTGGTCACAGACACGGTTTCCGGCGGTC AGAACGAAGATGCCCAGATCACACAGAGCACCATCTCTAGGTTCGCATGCAGGATCGTGTGTGACAGGAA CGAGCCATACACAGCACGCATATTTGCGGCAGGATTCGATTCTTCCAAAAATATCTTTCTTGGAGAGAAA GCAGCAAAATGGAAAAACCCTGATGGACACATGGATGGACTCACTACCAACGGTGTCCTGGTGATGCACC CGCAAGGAGGCTTCACCGAGGAATCCCAGCCTGGAGTCTGGAGAGAGATCTCTGTCTGTGGGGATGTGTA CACCTTGCGAGAGACCAGGTCGGCCCAGCAGAGGGGAAAGCTGGTGGAAAGTGAGACCAACGTCCTGCAA GACGGCTCCCTCATTGACCTGTGTGGGGCCACTCTCCTCTGGAGAACCGCAGATGGCCTTTTTCACGCTC CTACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATCAATGCAGCCCGACCCCAGTGCCCCGTGGGCCT TAACACCCTGGCCTTCCCCAGCATCAACCGGAAGGAAGTGGTGGAAGAGAAGCAGCCCTGGGCATACCTG AGCTGCGGCCATGTGCACGGCTACCACAGCTGGGGCCATCGGAGCGACGCGGAAGCCAACGAGAGGGAGT GTCCCATGTGCAGGACTGTGGGCCCCTACGTCCCTCTCTGGCTGGGCTGTGAGGCAGGATTTTATGTCGA TGCGGGACCCCCAACTCACGCTTTCACCCCCTGCGGGCACGTCTGTTCAGAGAAGTCTGCCAAGTACTGG TCGCAGATCCCACTGCCCCACGGAACGCACGCGTTTCACGCCGCCTGTCCGTTCTGCGCCACGCAGCTGG TTGGTGAACAGAACTGCATCAAATTGATTTTCCAAGGTCCAGTGGAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204612 representing BC027062
Red=Cloning site Green=Tags(s) MFQVGRSTESPIDFVVTDTVSGGQNEDAQITQSTISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLGEK AAKWKNPDGHMDGLTTNGVLVMHPQGGFTEESQPGVWREISVCGDVYTLRETRSAQQRGKLVESETNVLQ DGSLIDLCGATLLWRTADGLFHAPTQKHIEALRQEINAARPQCPVGLNTLAFPSINRKEVVEEKQPWAYL SCGHVHGYHSWGHRSDAEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPTHAFTPCGHVCSEKSAKYW SQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPVD SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027062 |
| ORF Size | 957 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027062.1 |
| RefSeq Size | 1732 bp |
| RefSeq ORF | 959 bp |
| Locus ID | 93834 |
| Gene Summary | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218511 | Peli2 (untagged) - Mouse pellino 2 (cDNA clone MGC:38603 IMAGE:5355240), (10ug) |
CNY 6,940.00 |
|
| MR204612 | Peli2 (Myc-DDK-tagged) - Mouse pellino 2 (cDNA clone MGC:38603 IMAGE:5355240) |
CNY 3,140.00 |
|
| MR204612L3 | Lenti ORF clone of Peli2 (Myc-DDK-tagged) - Mouse pellino 2 (cDNA clone MGC:38603 IMAGE:5355240) |
CNY 5,040.00 |
|
| MR204612L4 | Lenti ORF clone of Peli2 (mGFP-tagged) - Mouse pellino 2 (cDNA clone MGC:38603 IMAGE:5355240) |
CNY 5,040.00 |
