Qpct (BC020023) Mouse Tagged ORF Clone

CAT#: MG204454

  • TrueORF®

Qpct (tGFP-tagged) - Mouse glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (cDNA clone MGC:27858 IMAGE:3491756)

ORF Plasmid: DDK tGFP



  "BC020023" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 5730422A13Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204454 representing BC020023
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGCAGCGAAGACAAACGCGTCGTGGGCACTCTCCACCTGCTGCTACTGCAGGCGACCGTCCTGT
CTCTGACAGCTGGGAATCTGAATCTGGTCTCCGGTGCCTGGACGCAGGAGAAGCACATCATGCAACGAAT
TCAGAGACTTCAGGCTGAGTGGGTCGTGGAAGTTGACACCTTCCTGAGTAGGACTCCCTATGGCTATCGG
TCCTTCTCAAATATCATCAGCACTCTTAACCCGGAAGCGAAACGACACCTGGTCCTCGCCTGCCACTACG
ACTCCAAATATTTTCCTCGATGGGACAGCAGAGTGTTTGTGGGAGCCACGGATTCAGCTGTGCCATGTGC
AATGATGTTGGAACTTGCCCGTGCCTTAGACAAGAAACTCCATTCCTTGAAGGATGTCTCTGGTTCCAAG
CCAGATCTCTCACTCCGGCTAATTTTCTTTGATGGTGAAGAGGCTTTTCATCACTGGTCCCCTCAAGATT
CTCTGTATGGGTCTCGGCACTTAGCTCAGAAGATGGCATCAAGCCCTCACCCTCCTGGATCAAGAGGCAC
CAACCAACTGGATGGCATGGATCTGTTGGTCTTACTAGATTTAATTGGAGCAGCAAATCCAACATTCCCT
AATTTTTTCCCCAAGACTACCAGATGGTTTAATAGACTTCAAGCAATTGAAAAGGAACTCTATGAATTGG
GATTACTCAAGGATCATTCTTTGGAGAGGAAGTATTTTCAGAATTTTGGCTATGGAAATATTATCCAGGA
TGACCACATTCCATTTTTAAGAAAAGGTGTCCCAGTTCTTCACCTGATAGCTTCTCCTTTCCCTGAAGTC
TGGCACACCATGGATGACAATGAAGAAAATCTACATGCGTCAACCATTGACAATCTCAACAAAATCATTC
AAGTCTTTGTGTTGGAATATCTTCACTTG


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204454 representing BC020023
Red=Cloning site Green=Tags(s)

MAGSEDKRVVGTLHLLLLQATVLSLTAGNLNLVSGAWTQEKHIMQRIQRLQAEWVVEVDTFLSRTPYGYR
SFSNIISTLNPEAKRHLVLACHYDSKYFPRWDSRVFVGATDSAVPCAMMLELARALDKKLHSLKDVSGSK
PDLSLRLIFFDGEEAFHHWSPQDSLYGSRHLAQKMASSPHPPGSRGTNQLDGMDLLVLLDLIGAANPTFP
NFFPKTTRWFNRLQAIEKELYELGLLKDHSLERKYFQNFGYGNIIQDDHIPFLRKGVPVLHLIASPFPEV
WHTMDDNEENLHASTIDNLNKIIQVFVLEYLHL

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC020023
ORF Size 941 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC020023, AAH20023
RefSeq Size 1486 bp
RefSeq ORF 941 bp
Locus ID 70536
Gene Summary Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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