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Nek6 (NM_021606) Mouse Tagged ORF Clone
CAT＃: MG204452
- TrueORF®
Nek6 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6)
"NM_021606" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1300007C09Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204452 representing NM_021606
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGACAGCCCAGCCACATGCCTCATGGAGGGAGCCCAAACCACCTCTGCCATGCACTGGGGCCTG CACCCCCTCCAGACCCACAGAGGCTCCCAAACACCCTGTCTTTTCGCTGCTCACTGGCAGACTTCCAAAT TGAGAAGAAGATTGGCCGAGGACAGTTCAGTGAGGTTTACAAGGCCACTTGCCTGCTGGACAGGAAGACA GTGGCTCTGAAGAAGGTGCAGATCTTTGAGATGATGGATGCCAAAGCCAGGCAAGACTGTGTCAAGGAAA TTGGTCTCCTAAAGCAACTGAACCATCCGAATATCATCAAGTATCTGGACTCCTTCATCGAAGACAATGA GCTGAACATTGTGCTGGAGTTGGCTGACGCGGGTGACCTCTCACAGATGATCAAGTATTTCAAGAAGCAG AAGCGGCTTATCCCTGAGAGGACCGTGTGGAAGTACTTCGTGCAGCTGTGCAGCGCTGTGGAGCACATGC ACTCCCGACGTGTGATGCACCGAGACATCAAGCCCGCCAACGTGTTCATCACAGCTACGGGCATTGTGAA GCTTGGTGACCTCGGCCTGGGCCGCTTCTTCAGCTCGGAGACCACTGCGGCCCACTCACTAGTGGGAACA CCATACTACATGTCACCCGAGAGAATCCATGAGAATGGCTACAACTTCAAGTCAGACATCTGGTCCCTAG GCTGCCTGCTGTATGAGATGGCAGCTCTCCAGAGCCCCTTCTATGGAGATAAGATGAATCTCTTCTCCCT CTGCCAAAAGATTGAGCAGTGTGACTACCCGCCTCTCCCGGGAGAGCACTACTCTGAGAAGCTTCGGGAA CTGGTCAGTATGTGTATCTACCCTGACCCTGACCACCGACCTGACATTGTATATGTGCACCAGGTGGCTA GACAGATGCACGTCTGGACATCCAGCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204452 representing NM_021606
Red=Cloning site Green=Tags(s) MAGQPSHMPHGGSPNHLCHALGPAPPPDPQRLPNTLSFRCSLADFQIEKKIGRGQFSEVYKATCLLDRKT VALKKVQIFEMMDAKARQDCVKEIGLLKQLNHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQ KRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGIVKLGDLGLGRFFSSETTAAHSLVGT PYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSEKLRE LVSMCIYPDPDHRPDIVYVHQVARQMHVWTSST TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021606 |
| ORF Size | 3165 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021606.3, NP_067619.1 |
| RefSeq Size | 3724 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 59126 |
| UniProt ID | Q9ES70 |
| Gene Summary | Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217007 | Nek6 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6) transcript variant 2, (10ug) |
CNY 2,850.00 |
|
| MR217007 | Nek6 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), transcript variant 2 |
CNY 2,400.00 |
|
| MR217007L3 | Lenti ORF clone of Nek6 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), transcript variant 2 |
CNY 4,750.00 |
|
| MR217007L4 | Lenti ORF clone of Nek6 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), transcript variant 2 |
CNY 4,750.00 |
